Literature summary for 2.4.1.17 extracted from

Jinno, H.; Saeki, M.; Saito, Y.; Tanaka-Kagawa, T.; Hanioka, N.; Sai, K.; Kaniwa, N.; Ando, M.; Shirao, K.; Minami, H.; Ohtsu, A.; Yoshida, T.; Saijo, N.; Ozawa, S.; Sawada, J.
Functional characterization of human UDP-glucuronosyltransferase 1A9 variant, D256N, found in Japanese cancer patients (2003), J. Pharmacol. Exp. Ther., 306, 688-693.

Search for more literature for 2.4.1.17

Cloned(Commentary)

Cloned (Comment)	Organism
expression of wild-type and mutant D256N enzymes in COS-1 cells	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
additional information	mutant isozyme UGT1A9 D256N is isolated from a japanese cancer patient, site-directed mutagenesis for expression plasmid construction, the mutant shows less than 5% of wild-type isozyme activity with 7-ethyl-10-hydroxycampothecin as substrate, the naturally occuring mutant isozyme might be involved in irinotecan metabolism in vivo	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
19.3	-	7-ethyl-10-hydroxycampothecin	recombinant wild-type isozyme UGT1A9, pH.4, 37°C	Homo sapiens
44.4	-	7-ethyl-10-hydroxycampothecin	recombinant UGT1A9 mutant D256N isozyme, pH.4, 37°C	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
membrane	-	Homo sapiens	16020	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	-	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	O60656	isozymes UGT1A1, UGT1A7, UGT1A9, naturally occuring UGT1A9 mutant D256N, the latter from a japanese cancer patient	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
liver	isozyme UGT1A1 and UGT1A9	Homo sapiens	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	-	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	in contrast to isozymes UGT1A1 and UGT1A7, the isozyme UGT1A9 does not show polymorphism influencing the glucuronidation activity of SN-38	Homo sapiens	?	-	?
UDP-glucuronate + 7-ethyl-10-hydroxycampothecin	i.e. SN-38, isozymes UGT1A1, UGT1A7, and UGT1A9	Homo sapiens	UDP + 7-ethyl-10-hydroxycampothecin 1-O-beta-D-glucuronide	-	?

Synonyms

Synonyms	Comment	Organism
UDP-glucuronosyltransferase 1A9	-	Homo sapiens
UGT1A9	-	Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.4	-	assay at	Homo sapiens

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Release 2023.1 (January 2023)