BRENDA - Enzyme Database
show all sequences of 2.4.1.162

Synthesis of novel disaccharides by a newly isolated fructosyl transferase from Bacillus subtilis

Cheetham, P.S.; Hacking, A.J.; Vlitos, M.; Enzyme Microb. Technol. 11, 212-219 (1989)
No PubMed abstract available

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
additional information
the fructosyl transferase is unaffected by anionic, cationic, or nonionic detergents such as SDS, cetyltrimethylammonium bromide, and Triton X-100
Bacillus subtilis
sucrose
the fructosyl transferase activity in strain NCIB 11871 is induced only by sucrose, other sugars tested as inducers (glucose, fructose, maltose, and cellobiose) are ineffective
Bacillus subtilis
Inhibitors
Inhibitors
Commentary
Organism
Structure
D-glucose
-
Bacillus subtilis
D-mellibiose
-
Bacillus subtilis
additional information
p-hydroxymercuribenzoate does not affect fructosyl transferase activity, the fructosyl transferase is completely resistant to endogenous proteases and to trypsin
Bacillus subtilis
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
700
-
sucrose
using D-xylose as the acceptor sugar, at pH 5.4 and 30°C
Bacillus subtilis
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
additional information
no requirement for metal ions
Bacillus subtilis
Organism
Organism
UniProt
Commentary
Textmining
Bacillus subtilis
-
-
-
Bacillus subtilis NCIB 11871
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
ammonium sulfate precipitation, DEAE-52 cellulose column chromatography, and Mono Q column chromatography
Bacillus subtilis
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
the purified enzyme is concentrated 33fold and has an activity of 32 nmol/ml/min
Bacillus subtilis
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
1',6'-dichlorosucrose + fructose acceptor
1',6'-dichlorosucrose has some donor activity
692116
Bacillus subtilis
?
-
-
-
?
6,1',6'-trichlorosucrose + fructose acceptor
6, 1',6'-trichlorosucrose has some donor activity
692116
Bacillus subtilis
?
-
-
-
?
6,1',6'-trichlorosucrose + fructose acceptor
6, 1',6'-trichlorosucrose has some donor activity
692116
Bacillus subtilis NCIB 11871
?
-
-
-
?
6-chlorosucrose + fructose acceptor
-
692116
Bacillus subtilis
?
-
-
-
?
6-chlorosucrose + fructose acceptor
-
692116
Bacillus subtilis NCIB 11871
?
-
-
-
?
6-deoxysucrose + fructose acceptor
-
692116
Bacillus subtilis
?
-
-
-
?
6-deoxysucrose + fructose acceptor
-
692116
Bacillus subtilis NCIB 11871
?
-
-
-
?
D-sucrose + D-galactose 6-acetate
-
692116
Bacillus subtilis
D-glucose + beta-D-fructofuranosyl-(2,1)-D-6-O-acetyl-galactopyranoside
-
-
-
?
D-sucrose + D-galactose 6-phosphate
-
692116
Bacillus subtilis
?
-
-
-
?
D-sucrose + D-gluconic acid
-
692116
Bacillus subtilis
D-glucose + beta-D-fructofuranosyl-(2,1)-D-gluconic acid
-
-
-
?
galactosucrose + fructose acceptor
-
692116
Bacillus subtilis
?
-
-
-
?
additional information
when oligosaccharide acceptors are used, the acceptor activity decreases with increasing size, as in the homologous series maltose, maltotriose, maltotetrose, etc., reactivity also decreases with the size of the molecule in the homologous series sucrose, raffinose, stachyose, and verbascose, inulin and levan, and also levan that is partially hydrolysed with acid, do not act as donors, lactulose, isomaltulose, D-glucose-1-phosphate, methyl beta-D-glucoside, and D-xylulose are unreactive
692116
Bacillus subtilis
?
-
-
-
?
additional information
when oligosaccharide acceptors are used, the acceptor activity decreases with increasing size, as in the homologous series maltose, maltotriose, maltotetrose, etc., reactivity also decreases with the size of the molecule in the homologous series sucrose, raffinose, stachyose, and verbascose, inulin and levan, and also levan that is partially hydrolysed with acid, do not act as donors, lactulose, isomaltulose, D-glucose-1-phosphate, methyl beta-D-glucoside, and D-xylulose are unreactive
692116
Bacillus subtilis NCIB 11871
?
-
-
-
?
raffinose + D-xylose
-
692116
Bacillus subtilis
beta-D-fructofuranosyl-2,1-alpha-D-xylopyranoside + alpha-D-galactopyranosyl alpha-D-glucopyranoside
-
-
-
?
stachyose + fructose acceptor
-
692116
Bacillus subtilis
?
-
-
-
?
sucrose + 1-thio-D-glucose
-
692116
Bacillus subtilis
?
-
-
-
?
sucrose + 3-O-methyl-D-glucose
-
692116
Bacillus subtilis
D-glucose + beta-D-fructofuranosyl-(2,1)-3-O-methyl-D-glucoside
-
-
-
?
sucrose + 4-chloro-D-galactose
-
692116
Bacillus subtilis
?
-
-
-
?
sucrose + 5-thio-D-glucose
-
692116
Bacillus subtilis
?
-
-
-
?
sucrose + 6-chloro-6-deoxy-D-glucose
-
692116
Bacillus subtilis
D-glucose + beta-D-fructofuranosyl-(2,1)-6-chloro-6-deoxy-D-glucoside
-
-
-
?
sucrose + 6-deoxy-D-glucose
-
692116
Bacillus subtilis
6-deoxysucrose + D-glucose
-
-
-
?
sucrose + 6-O-benzyl-D-glucose
-
692116
Bacillus subtilis
D-glucose + beta-D-fructofuranosyl-(2,1)-6-O-benzyl-D-glucoside
-
-
-
?
sucrose + 6-O-methyl-D-galactose
-
692116
Bacillus subtilis
D-glucose + beta-D-fructofuranosyl-(2,1)-6-O-methyl-D-galactoside
-
-
-
?
sucrose + 6-O-methyl-D-glucose
-
692116
Bacillus subtilis
D-glucose + beta-D-fructofuranosyl-(2,1)-6-O-methyl-D-glucoside
-
-
-
?
sucrose + cellobiose
-
692116
Bacillus subtilis
D-glucose + beta-D-fructofuranosyl-(2,1)-cellobioside
-
-
-
?
sucrose + D-arabinose
-
692116
Bacillus subtilis
D-glucose + beta-D-fructofuranosyl-(2,1)-D-arabinoside
-
-
-
?
sucrose + D-fucose
-
692116
Bacillus subtilis
D-glucose + beta-D-fructofuranosyl-(2,1)-D-fucoside
-
-
-
?
sucrose + D-galactose
-
692116
Bacillus subtilis
D-glucose + beta-D-fructofuranosyl-(2,1)-D-galactopyranoside
-
-
-
?
sucrose + D-glucose 6-acetate
-
692116
Bacillus subtilis
sucrose 6-acetate + D-glucose
-
-
-
?
sucrose + D-glucose 6-phosphate
-
692116
Bacillus subtilis
sucrose 6-phosphate + D-glucose
-
-
-
?
sucrose + D-lactose
-
692116
Bacillus subtilis
D-glucose + beta-D-fructofuranosyl-(2,1)-D-lactoside
-
-
-
?
sucrose + D-lyxose
-
692116
Bacillus subtilis
D-glucose + beta-D-fructofuranosyl-(2,1)-D-lyxoside
-
-
-
?
sucrose + D-maltose
-
692116
Bacillus subtilis
D-glucose + beta-D-fructofuranosyl-(2,1)-D-maltoside
-
-
-
?
sucrose + D-mannose
-
692116
Bacillus subtilis
D-glucose + beta-D-fructofuranosyl-(2,1)-D-mannopyranoside
-
-
-
?
sucrose + D-ribose
-
692116
Bacillus subtilis
D-glucose + beta-D-fructofuranosyl-(2,1)-D-riboside
-
-
-
?
sucrose + D-xylose
-
692116
Bacillus subtilis
D-glucose + beta-D-fructofuranosyl-(2,1)-alpha-D-xylopyranoside
-
-
-
?
sucrose + D-xylose
-
692116
Bacillus subtilis
D-glucose + beta-D-fructofuranosyl-2,1-alpha-D-xylopyranoside
-
-
-
?
sucrose + ethyl maltol
-
692116
Bacillus subtilis
?
-
-
-
?
sucrose + glycerol
-
692116
Bacillus subtilis
?
-
-
-
?
sucrose + isomaltose
-
692116
Bacillus subtilis
D-glucose + beta-D-fructofuranosyl-(2,1)-isomaltoside
-
-
-
?
sucrose + L-arabinose
-
692116
Bacillus subtilis
D-glucose + beta-D-fructofuranosyl-(2,1)-L-arabinoside
-
-
-
?
sucrose + L-rhamnose
-
692116
Bacillus subtilis
D-glucose + beta-D-fructofuranosyl-(2,1)-L-rhamnoside
-
-
-
?
sucrose + L-sorbose
-
692116
Bacillus subtilis
?
-
-
-
?
sucrose + maltohexaose
-
692116
Bacillus subtilis
D-glucose + beta-D-fructofuranosyl-(2,1)-maltohexaoside
-
-
-
?
sucrose + maltol
-
692116
Bacillus subtilis
?
-
-
-
?
sucrose + maltopentaose
-
692116
Bacillus subtilis
D-glucose + beta-D-fructofuranosyl-(2,1)-maltopentaoside
-
-
-
?
sucrose + maltotetraose
-
692116
Bacillus subtilis
D-glucose + beta-D-fructofuranosyl-(2,1)-maltotetraoside
-
-
-
?
sucrose + maltulose
-
692116
Bacillus subtilis
?
-
-
-
?
sucrose + melibiose
-
692116
Bacillus subtilis
D-glucose + beta-D-fructofuranosyl-(2,1)-mellibioside
-
-
-
?
sucrose + xylitol
-
692116
Bacillus subtilis
?
-
-
-
?
sucrose + xylitol
-
692116
Bacillus subtilis NCIB 11871
?
-
-
-
?
sucrose 6-acetate + fructose acceptor
-
692116
Bacillus subtilis
?
-
-
-
?
verbascose + fructose acceptor
-
692116
Bacillus subtilis
?
-
-
-
?
xylosucrose + fructose acceptor
-
692116
Bacillus subtilis
?
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
beta-fructofuranoside, acceptor (aldose) fructosyltransferase
-
Bacillus subtilis
beta-fructosyl transferase
-
Bacillus subtilis
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
40
-
the enzyme is stable up to 40°C
Bacillus subtilis
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5
-
-
Bacillus subtilis
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
additional information
the fructosyl transferase is unaffected by anionic, cationic, or nonionic detergents such as SDS, cetyltrimethylammonium bromide, and Triton X-100
Bacillus subtilis
sucrose
the fructosyl transferase activity in strain NCIB 11871 is induced only by sucrose, other sugars tested as inducers (glucose, fructose, maltose, and cellobiose) are ineffective
Bacillus subtilis
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
D-glucose
-
Bacillus subtilis
D-mellibiose
-
Bacillus subtilis
additional information
p-hydroxymercuribenzoate does not affect fructosyl transferase activity, the fructosyl transferase is completely resistant to endogenous proteases and to trypsin
Bacillus subtilis
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
700
-
sucrose
using D-xylose as the acceptor sugar, at pH 5.4 and 30°C
Bacillus subtilis
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
additional information
no requirement for metal ions
Bacillus subtilis
Purification (Commentary) (protein specific)
Commentary
Organism
ammonium sulfate precipitation, DEAE-52 cellulose column chromatography, and Mono Q column chromatography
Bacillus subtilis
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
the purified enzyme is concentrated 33fold and has an activity of 32 nmol/ml/min
Bacillus subtilis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
1',6'-dichlorosucrose + fructose acceptor
1',6'-dichlorosucrose has some donor activity
692116
Bacillus subtilis
?
-
-
-
?
6,1',6'-trichlorosucrose + fructose acceptor
6, 1',6'-trichlorosucrose has some donor activity
692116
Bacillus subtilis
?
-
-
-
?
6,1',6'-trichlorosucrose + fructose acceptor
6, 1',6'-trichlorosucrose has some donor activity
692116
Bacillus subtilis NCIB 11871
?
-
-
-
?
6-chlorosucrose + fructose acceptor
-
692116
Bacillus subtilis
?
-
-
-
?
6-chlorosucrose + fructose acceptor
-
692116
Bacillus subtilis NCIB 11871
?
-
-
-
?
6-deoxysucrose + fructose acceptor
-
692116
Bacillus subtilis
?
-
-
-
?
6-deoxysucrose + fructose acceptor
-
692116
Bacillus subtilis NCIB 11871
?
-
-
-
?
D-sucrose + D-galactose 6-acetate
-
692116
Bacillus subtilis
D-glucose + beta-D-fructofuranosyl-(2,1)-D-6-O-acetyl-galactopyranoside
-
-
-
?
D-sucrose + D-galactose 6-phosphate
-
692116
Bacillus subtilis
?
-
-
-
?
D-sucrose + D-gluconic acid
-
692116
Bacillus subtilis
D-glucose + beta-D-fructofuranosyl-(2,1)-D-gluconic acid
-
-
-
?
galactosucrose + fructose acceptor
-
692116
Bacillus subtilis
?
-
-
-
?
additional information
when oligosaccharide acceptors are used, the acceptor activity decreases with increasing size, as in the homologous series maltose, maltotriose, maltotetrose, etc., reactivity also decreases with the size of the molecule in the homologous series sucrose, raffinose, stachyose, and verbascose, inulin and levan, and also levan that is partially hydrolysed with acid, do not act as donors, lactulose, isomaltulose, D-glucose-1-phosphate, methyl beta-D-glucoside, and D-xylulose are unreactive
692116
Bacillus subtilis
?
-
-
-
?
additional information
when oligosaccharide acceptors are used, the acceptor activity decreases with increasing size, as in the homologous series maltose, maltotriose, maltotetrose, etc., reactivity also decreases with the size of the molecule in the homologous series sucrose, raffinose, stachyose, and verbascose, inulin and levan, and also levan that is partially hydrolysed with acid, do not act as donors, lactulose, isomaltulose, D-glucose-1-phosphate, methyl beta-D-glucoside, and D-xylulose are unreactive
692116
Bacillus subtilis NCIB 11871
?
-
-
-
?
raffinose + D-xylose
-
692116
Bacillus subtilis
beta-D-fructofuranosyl-2,1-alpha-D-xylopyranoside + alpha-D-galactopyranosyl alpha-D-glucopyranoside
-
-
-
?
stachyose + fructose acceptor
-
692116
Bacillus subtilis
?
-
-
-
?
sucrose + 1-thio-D-glucose
-
692116
Bacillus subtilis
?
-
-
-
?
sucrose + 3-O-methyl-D-glucose
-
692116
Bacillus subtilis
D-glucose + beta-D-fructofuranosyl-(2,1)-3-O-methyl-D-glucoside
-
-
-
?
sucrose + 4-chloro-D-galactose
-
692116
Bacillus subtilis
?
-
-
-
?
sucrose + 5-thio-D-glucose
-
692116
Bacillus subtilis
?
-
-
-
?
sucrose + 6-chloro-6-deoxy-D-glucose
-
692116
Bacillus subtilis
D-glucose + beta-D-fructofuranosyl-(2,1)-6-chloro-6-deoxy-D-glucoside
-
-
-
?
sucrose + 6-deoxy-D-glucose
-
692116
Bacillus subtilis
6-deoxysucrose + D-glucose
-
-
-
?
sucrose + 6-O-benzyl-D-glucose
-
692116
Bacillus subtilis
D-glucose + beta-D-fructofuranosyl-(2,1)-6-O-benzyl-D-glucoside
-
-
-
?
sucrose + 6-O-methyl-D-galactose
-
692116
Bacillus subtilis
D-glucose + beta-D-fructofuranosyl-(2,1)-6-O-methyl-D-galactoside
-
-
-
?
sucrose + 6-O-methyl-D-glucose
-
692116
Bacillus subtilis
D-glucose + beta-D-fructofuranosyl-(2,1)-6-O-methyl-D-glucoside
-
-
-
?
sucrose + cellobiose
-
692116
Bacillus subtilis
D-glucose + beta-D-fructofuranosyl-(2,1)-cellobioside
-
-
-
?
sucrose + D-arabinose
-
692116
Bacillus subtilis
D-glucose + beta-D-fructofuranosyl-(2,1)-D-arabinoside
-
-
-
?
sucrose + D-fucose
-
692116
Bacillus subtilis
D-glucose + beta-D-fructofuranosyl-(2,1)-D-fucoside
-
-
-
?
sucrose + D-galactose
-
692116
Bacillus subtilis
D-glucose + beta-D-fructofuranosyl-(2,1)-D-galactopyranoside
-
-
-
?
sucrose + D-glucose 6-acetate
-
692116
Bacillus subtilis
sucrose 6-acetate + D-glucose
-
-
-
?
sucrose + D-glucose 6-phosphate
-
692116
Bacillus subtilis
sucrose 6-phosphate + D-glucose
-
-
-
?
sucrose + D-lactose
-
692116
Bacillus subtilis
D-glucose + beta-D-fructofuranosyl-(2,1)-D-lactoside
-
-
-
?
sucrose + D-lyxose
-
692116
Bacillus subtilis
D-glucose + beta-D-fructofuranosyl-(2,1)-D-lyxoside
-
-
-
?
sucrose + D-maltose
-
692116
Bacillus subtilis
D-glucose + beta-D-fructofuranosyl-(2,1)-D-maltoside
-
-
-
?
sucrose + D-mannose
-
692116
Bacillus subtilis
D-glucose + beta-D-fructofuranosyl-(2,1)-D-mannopyranoside
-
-
-
?
sucrose + D-ribose
-
692116
Bacillus subtilis
D-glucose + beta-D-fructofuranosyl-(2,1)-D-riboside
-
-
-
?
sucrose + D-xylose
-
692116
Bacillus subtilis
D-glucose + beta-D-fructofuranosyl-(2,1)-alpha-D-xylopyranoside
-
-
-
?
sucrose + D-xylose
-
692116
Bacillus subtilis
D-glucose + beta-D-fructofuranosyl-2,1-alpha-D-xylopyranoside
-
-
-
?
sucrose + ethyl maltol
-
692116
Bacillus subtilis
?
-
-
-
?
sucrose + glycerol
-
692116
Bacillus subtilis
?
-
-
-
?
sucrose + isomaltose
-
692116
Bacillus subtilis
D-glucose + beta-D-fructofuranosyl-(2,1)-isomaltoside
-
-
-
?
sucrose + L-arabinose
-
692116
Bacillus subtilis
D-glucose + beta-D-fructofuranosyl-(2,1)-L-arabinoside
-
-
-
?
sucrose + L-rhamnose
-
692116
Bacillus subtilis
D-glucose + beta-D-fructofuranosyl-(2,1)-L-rhamnoside
-
-
-
?
sucrose + L-sorbose
-
692116
Bacillus subtilis
?
-
-
-
?
sucrose + maltohexaose
-
692116
Bacillus subtilis
D-glucose + beta-D-fructofuranosyl-(2,1)-maltohexaoside
-
-
-
?
sucrose + maltol
-
692116
Bacillus subtilis
?
-
-
-
?
sucrose + maltopentaose
-
692116
Bacillus subtilis
D-glucose + beta-D-fructofuranosyl-(2,1)-maltopentaoside
-
-
-
?
sucrose + maltotetraose
-
692116
Bacillus subtilis
D-glucose + beta-D-fructofuranosyl-(2,1)-maltotetraoside
-
-
-
?
sucrose + maltulose
-
692116
Bacillus subtilis
?
-
-
-
?
sucrose + melibiose
-
692116
Bacillus subtilis
D-glucose + beta-D-fructofuranosyl-(2,1)-mellibioside
-
-
-
?
sucrose + xylitol
-
692116
Bacillus subtilis
?
-
-
-
?
sucrose + xylitol
-
692116
Bacillus subtilis NCIB 11871
?
-
-
-
?
sucrose 6-acetate + fructose acceptor
-
692116
Bacillus subtilis
?
-
-
-
?
verbascose + fructose acceptor
-
692116
Bacillus subtilis
?
-
-
-
?
xylosucrose + fructose acceptor
-
692116
Bacillus subtilis
?
-
-
-
?
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
40
-
the enzyme is stable up to 40°C
Bacillus subtilis
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5
-
-
Bacillus subtilis
Other publictions for EC 2.4.1.162
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
760080
Gabrielczyk
Effects of ionic strength on ...
Bacillus subtilis, Bacillus subtilis NCIMB 11871
Protein Expr. Purif.
130
100-106
2017
-
-
1
-
-
-
-
-
-
-
-
-
-
8
-
-
1
-
1
-
-
-
-
1
3
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
760082
Gabrielczyk
Ion exchange resins as additi ...
Bacillus subtilis, Bacillus subtilis NCIMB 11871
Protein Expr. Purif.
133
35-40
2017
-
-
1
-
-
-
-
-
-
-
-
-
-
8
-
-
1
-
1
-
-
-
-
1
3
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
665777
Baciu
Investigations of the transfru ...
Bacillus subtilis, Bacillus subtilis 168
J. Biotechnol.
116
347-357
2004
-
-
-
-
-
-
-
-
-
-
-
-
-
100
-
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
692116
Cheetham
-
Synthesis of novel disaccharid ...
Bacillus subtilis, Bacillus subtilis NCIB 11871
Enzyme Microb. Technol.
11
212-219
1989
2
-
-
-
-
-
3
1
-
1
-
-
-
2
-
-
1
-
-
-
1
-
54
-
2
-
-
1
-
1
-
-
-
-
-
-
2
-
-
-
-
-
-
-
3
-
1
-
1
-
-
-
-
-
1
-
-
1
-
54
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
488677
Rathbone
-
Trihalo-trideoxy galactosucros ...
Bacillus subtilis, Erwinia sp.
UK Patent Application
GB21
45080 A
1985
-
-
-
-
-
-
-
1
2
-
-
2
-
2
-
-
1
-
-
-
-
-
83
-
-
1
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
2
-
-
2
-
-
-
1
-
-
-
-
83
-
1
-
2
-
1
-
-
-
-
-
-
-
-
-