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Literature summary for 2.4.1.16 extracted from

  • Salgado-Lugo, H.; Sanchez-Arreguin, A.; Ruiz-Herrera, J.
    Heterologous expression of an active chitin synthase from Rhizopus oryzae (2016), Fungal Genet. Biol., 97, 10-17 .
    View publication on PubMed
Search for more literature for 2.4.1.16

Activating Compound

Activating Compound Comment Organism Structure
N-acetyl-D-glucosamine 1.28fold stimulation of activity at 20 mM Rhizopus arrhizus

Cloned(Commentary)

Cloned (Comment) Organism
N-terminally deleted enzyme is expressed in Escherichia coli BL21 Star (DE3) cells Rhizopus arrhizus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
32.1
-
 UDP-N-acetyl-D-glucosamine at pH 6.5 and 25°C Rhizopus arrhizus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ may replace the activating effect of Mg2+ Rhizopus arrhizus
Co2+ may replace the activating effect of Mg2+ Rhizopus arrhizus
Mg2+ 5 mM used in assay conditions Rhizopus arrhizus
Mn2+ may replace the activating effect of Mg2+ Rhizopus arrhizus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-N-acetyl-alpha-D-glucosamine + [(1->4)-N-acetyl-beta-D-glucosaminyl]n Rhizopus arrhizus
-
 UDP + [(1->4)-N-acetyl-beta-D-glucosaminyl]n+1
-
 ?

Organism

Organism UniProt Comment Textmining
Rhizopus arrhizus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
nickel affinity column chromatography Rhizopus arrhizus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-N-acetyl-alpha-D-glucosamine + [(1->4)-N-acetyl-beta-D-glucosaminyl]n
-
 Rhizopus arrhizus UDP + [(1->4)-N-acetyl-beta-D-glucosaminyl]n+1
-
 ?
UDP-N-acetyl-D-glucosamine + N-acetyl-D-glucosamine
-
 Rhizopus arrhizus UDP + 1,4-(N-acetyl-beta-D-glucosaminyl)2
-
 ?

Subunits

Subunits Comment Organism
? x * 57000, native enzyme, SDS-PAGE Rhizopus arrhizus
? x * 55000, recombinant enzyme, SDS-PAGE Rhizopus arrhizus

Synonyms

Synonyms Comment Organism
Chs1
-
 Rhizopus arrhizus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
15 40 the enzyme is active between 15 and 40°C Rhizopus arrhizus

pH Range

pH Minimum pH Maximum Comment Organism
5 8 the enzyme is active between pH 5.0 and 8.0 Rhizopus arrhizus