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Literature summary for 2.4.1.16 extracted from

  • Maue, L.; Meissner, D.; Merzendorfer, H.
    Purification of an active, oligomeric chitin synthase complex from the midgut of the tobacco hornworm (2009), Insect Biochem. Mol. Biol., 39, 654-659.
    View publication on PubMed

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
52000
-
PAGE Manduca sexta
60000
-
x * 174500, calculated, x * 190000 + x * 119000 + x * 60000, SDS-PAGE Manduca sexta
119000
-
x * 174500, calculated, x * 190000 + x * 119000 + x * 60000, SDS-PAGE Manduca sexta
174500
-
x * 174500, calculated, x * 190000 + x * 119000 + x * 60000, SDS-PAGE Manduca sexta
190000
-
x * 174500, calculated, x * 190000 + x * 119000 + x * 60000, SDS-PAGE Manduca sexta
440000 670000 gel filtration Manduca sexta

Organism

Organism UniProt Comment Textmining
Manduca sexta
-
isoform CHS2
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein purified chitin synthase complex can be stained with Calcofluor White suggesting that it is either glycosylated, or still associated with chitin fibrils Manduca sexta

Purification (Commentary)

Purification (Comment) Organism
-
Manduca sexta

Source Tissue

Source Tissue Comment Organism Textmining
midgut located at the apical tips of the brush border microvilli Manduca sexta
-

Subunits

Subunits Comment Organism
oligomer x * 174500, calculated, x * 190000 + x * 119000 + x * 60000, SDS-PAGE Manduca sexta