Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Digitonin | chitin synthase 2 is maximally stimulated at the sharply defined digitonin to protein ratio of 0.042. Chitin synthase I is maximally active at a digitonin to protein ratio of 0.3-0.75 | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Co2+ | strongly inhibits chitin synthase 1 | Saccharomyces cerevisiae | |
EDTA | inhibition of chitin synthase 1 and 2 | Saccharomyces cerevisiae | |
Mn2+ | 2.5 mM, 50% inhibition of chitin synthase 1 and 2 | Saccharomyces cerevisiae | |
Polyoxin D | competitive | Saccharomyces cerevisiae | |
Zn2+ | 2.5 mM, complete inhibition of both chitin synthase 1 and 2 | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.5 | - |
UDP-GlcNAc | chitin synthase 1 | Saccharomyces cerevisiae | |
0.6 | 0.8 | UDP-GlcNAc | chitin synthase 2 | Saccharomyces cerevisiae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | the best divalent cation stimulator of chitin synthase 1 and 2 | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-N-acetyl-D-glucosamine + [1,4-(N-acetyl-beta-D-glucosaminyl)]n | Saccharomyces cerevisiae | chitin synthetase 2 is responsible for chitin synthesis in vivo, chitin synthase 1 is not essential | UDP + [1,4-(N-acetyl-beta-D-glucosaminyl)]n+1 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
chitin synthetase 1 and 2 | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | chitin synthetase I exists as zymogen and is only measurable after trypsin treatment | Saccharomyces cerevisiae |
proteolytic modification | chitin synthetase 2 is not activated by proteolysis | Saccharomyces cerevisiae |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | chitin synthase II shows highest specific activities in extracts from logarithmically growing cultures. Chitin synthetase I, whether from growing cultures or stationary phase cultures, is only measurable after trypsin treatment, and levels of zymogen do not change | Saccharomyces cerevisiae | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-N-acetyl-D-glucosamine + [1,4-(N-acetyl-beta-D-glucosaminyl)]n | - |
Saccharomyces cerevisiae | UDP + [1,4-(N-acetyl-beta-D-glucosaminyl)]n+1 | - |
? | |
UDP-N-acetyl-D-glucosamine + [1,4-(N-acetyl-beta-D-glucosaminyl)]n | chitin synthetase 2 is responsible for chitin synthesis in vivo, chitin synthase 1 is not essential | Saccharomyces cerevisiae | UDP + [1,4-(N-acetyl-beta-D-glucosaminyl)]n+1 | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
chitin synthase 1 | Saccharomyces cerevisiae |
40 | - |
chitin synthase 2 | Saccharomyces cerevisiae |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 40 | 20°C: about 80% of maximal activity, 40°C: about 55% of maximal activity | Saccharomyces cerevisiae |
25 | 50 | 25°C: about 60% of maximal activity, 50°C: about 90% of maximal activity, chitin synthase 1 | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
chitin synthase 1 | Saccharomyces cerevisiae |
8 | - |
chitin synthase 2 | Saccharomyces cerevisiae |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.001 | - |
Polyoxin D | - |
Saccharomyces cerevisiae |