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Literature summary for 2.4.1.159 extracted from

  • Kleinehollenhorst, G.; Behrens, H.; Pegels, G.; Strunk, N.; Wiermann, R.
    Formation of flavonol 3-O-diglycosides and flavonol 3-O-triglycosides by enzyme extracts from anthers of Tulipa cv apeldoorn - characterization and activity of 3 different O-glycosyltransferases during anther development (1982), Z. Naturforsch. C, 37c, 587-599.
No PubMed abstract available
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Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol slight stimulation Tulipa hybrid cultivar
dithioerythritol slight stimulation Tulipa hybrid cultivar
glutathione slight stimulation Tulipa hybrid cultivar
additional information no stimulation by sucrose or bovine serum albumin Tulipa hybrid cultivar

Inhibitors

Inhibitors Comment Organism Structure
p-chloromercuribenzoate weak Tulipa hybrid cultivar

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ slight stimulation Tulipa hybrid cultivar
additional information no activation by Ca2+, NH4+, Mn2+, Cl- or SO42- Tulipa hybrid cultivar

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
 gel filtration Tulipa hybrid cultivar

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDPrhamnose + flavonol 3-O-glucoside Tulipa hybrid cultivar involved in flavonoid metabolism, pathway of flavonol 3-O-triglycoside biosynthesis UDP + flavonol 3-O-rhamnosylglucoside
-
 ?

Organism

Organism UniProt Comment Textmining
Tulipa hybrid cultivar
-
 tulip, cv. Apeldoorn
-

Purification (Commentary)

Purification (Comment) Organism
partial Tulipa hybrid cultivar

Source Tissue

Source Tissue Comment Organism Textmining
anther
-
 Tulipa hybrid cultivar
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDPrhamnose + flavonol 3-O-diglycoside
-
 Tulipa hybrid cultivar UDP + flavonol 3-O-triglycoside
-
 ?
UDPrhamnose + flavonol 3-O-glucoside acceptor substrates are 3-O-glucosides of quercetin, isorhamnetin or kaempferol, rutin, less efficient acceptors: quercetin 3-O-galactoside or quercetin 3-O-rhamnoside, kaempferol 3-O-rhamnoside or myrecetin 3-O-rhamnoside, no substrates: aglycones Tulipa hybrid cultivar UDP + flavonol 3-O-rhamnosylglucoside i.e. 3-O-rutinosides ?
UDPrhamnose + flavonol 3-O-glucoside involved in flavonoid metabolism, pathway of flavonol 3-O-triglycoside biosynthesis Tulipa hybrid cultivar UDP + flavonol 3-O-rhamnosylglucoside
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5 9
-
 Tulipa hybrid cultivar