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Literature summary for 2.4.1.155 extracted from

  • Abdel Rahman, A.; Ryczko, M.; Nakano, M.; Pawling, J.; Rodrigues, T.; Johswich, A.; Taniguchi, N.; Dennis, J.
    Golgi N-glycan branching N-acetylglucosaminyltransferases I, V and VI promote nutrient uptake and metabolism (2015), Glycobiology, 25, 225-240.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.1.155

Cloned(Commentary)

Cloned (Comment) Organism
gene MGAT5, recombinant tet-inducible expression of Flag-tagged GlcNAcT-V in HeLa and HEK293 cells. Tet-induced Mgat5 HEK293 cells display a 2fold increase in tri-antennary and a 2fold increase in tetraantennary N-glycans Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information GlcNAc and tet-induced Mgat5 increases the levels of many metabolites in HeLa cells, and HEK293 cells, and tet-induced Mgat5 changes in the N-glycan distributions, N-glycans profiles of transgenic HeLa and HEK-293 cells, overview. Tet-inducible Mgat5 enhances amino acid uptake and growth in nutrient-poor conditions and complex-type N-glycan branching in HEK293 Mgat5 clones. Up-regulation of Mgat5 in HEK293 cells stimulates amino acid uptake and increases metabolite levels under low Gln/Glc culture conditions Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-N-acetyl-D-glucosamine + 6-(2-[N-acetyl-beta-D-glucosaminyl]-alpha-D-mannosyl)-beta-D-mannosyl-R Homo sapiens
-
 UDP + 6-(2,6-bis[N-acetyl-beta-D-glucosaminyl]-alpha-D-mannosyl)-beta-D-mannosyl-R
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q09328 gene MGAT5
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-N-acetyl-D-glucosamine + 6-(2-[N-acetyl-beta-D-glucosaminyl]-alpha-D-mannosyl)-beta-D-mannosyl-R
-
 Homo sapiens UDP + 6-(2,6-bis[N-acetyl-beta-D-glucosaminyl]-alpha-D-mannosyl)-beta-D-mannosyl-R
-
 ?

Synonyms

Synonyms Comment Organism
GnT-V
-
 Homo sapiens
Mgat5
-
 Homo sapiens
N-acetylglucosaminyltransferase V
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
 assay at Homo sapiens

General Information

General Information Comment Organism
metabolism the enzyme is involved in the N-glycan-branching pathway, regulation of cellular metabolite levels by Mgat5, overview Homo sapiens