Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.4.1.152 extracted from

  • Choi, Y.H.; Kim, J.H.; Park, B.S.; Kim, B.G.
    Solubilization and iterative saturation mutagenesis of alpha1,3-fucosyltransferase from Helicobacter pylori to enhance its catalytic efficiency (2016), Biotechnol. Bioeng., 113, 1666-1675.
    View publication on PubMed
Search for more literature for 2.4.1.152

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BW25113 (DE3) cells Helicobacter pylori

Protein Variants

Protein Variants Comment Organism
A128N the mutant displays 3.4fold higher catalytic activity with D-lactose than the wild type enzyme Helicobacter pylori
A128N/H129E the mutant displays 6.5fold higher catalytic activity with D-lactose than the wild type enzyme Helicobacter pylori

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.24
-
 GDP-beta-L-fucose mutant enzyme A128N/H129E/Y132I, at pH 7.6 and 37°C Helicobacter pylori
0.28
-
 GDP-beta-L-fucose mutant enzyme A128N/H129E/S46F, at pH 7.6 and 37°C Helicobacter pylori
0.3
-
 GDP-beta-L-fucose mutant enzyme A128N/H129E/Y132I/S46F, at pH 7.6 and 37°C Helicobacter pylori
0.33
-
 GDP-beta-L-fucose wild type enzyme, at pH 7.6 and 37°C Helicobacter pylori
0.37
-
 GDP-beta-L-fucose mutant enzyme A128N, at pH 7.6 and 37°C Helicobacter pylori
0.37
-
 GDP-beta-L-fucose mutant enzyme A128N/H129E, at pH 7.6 and 37°C Helicobacter pylori
72
-
 D-lactose mutant enzyme A128N/H129E/Y132I/S46F, at pH 7.6 and 37°C Helicobacter pylori
81
-
 D-lactose mutant enzyme A128N/H129E/S46F, at pH 7.6 and 37°C Helicobacter pylori
97
-
 D-lactose mutant enzyme A128N/H129E/Y132I, at pH 7.6 and 37°C Helicobacter pylori
123
-
 D-lactose mutant enzyme A128N/H129E, at pH 7.6 and 37°C Helicobacter pylori
232
-
 D-lactose mutant enzyme A128N, at pH 7.6 and 37°C Helicobacter pylori
543
-
 D-lactose wild type enzyme, at pH 7.6 and 37°C Helicobacter pylori

Organism

Organism UniProt Comment Textmining
Helicobacter pylori
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GDP-beta-L-fucose + D-lactose the enzyme has very low relative substrate specificity toward D-lactose Helicobacter pylori GDP + 3 L-Fuc-alpha-(1->3)-[D-Gal-beta(1->4)]-D-Glc
-
 ?
GDP-beta-L-fucose + N-acetyl-D-lactosamine
-
 Helicobacter pylori GDP + L-Fuc-alpha-(1->3)-[D-Gal-beta(1->4)]-D-GlcNac
-
 ?

Synonyms

Synonyms Comment Organism
alpha1,3-fucosyltransferase
-
 Helicobacter pylori
alpha1,3-FucT
-
 Helicobacter pylori
FutA
-
 Helicobacter pylori

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.063
-
 GDP-beta-L-fucose wild type enzyme, at pH 7.6 and 37°C Helicobacter pylori
0.113
-
 GDP-beta-L-fucose mutant enzyme A128N, at pH 7.6 and 37°C Helicobacter pylori
0.417
-
 GDP-beta-L-fucose mutant enzyme A128N/H129E, at pH 7.6 and 37°C Helicobacter pylori
0.478
-
 GDP-beta-L-fucose mutant enzyme A128N/H129E/Y132I, at pH 7.6 and 37°C Helicobacter pylori
1.328
-
 GDP-beta-L-fucose mutant enzyme A128N/H129E/S46F, at pH 7.6 and 37°C Helicobacter pylori
1.512
-
 GDP-beta-L-fucose mutant enzyme A128N/H129E/Y132I/S46F, at pH 7.6 and 37°C Helicobacter pylori
2.25
-
 D-lactose wild type enzyme, at pH 7.6 and 37°C Helicobacter pylori
2.85
-
 D-lactose mutant enzyme A128N, at pH 7.6 and 37°C Helicobacter pylori
4
-
 D-lactose mutant enzyme A128N/H129E/Y132I, at pH 7.6 and 37°C Helicobacter pylori
4.03
-
 D-lactose mutant enzyme A128N/H129E, at pH 7.6 and 37°C Helicobacter pylori
5.62
-
 D-lactose mutant enzyme A128N/H129E/S46F, at pH 7.6 and 37°C Helicobacter pylori
5.97
-
 D-lactose mutant enzyme A128N/H129E/Y132I/S46F, at pH 7.6 and 37°C Helicobacter pylori

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.004
-
 D-lactose wild type enzyme, at pH 7.6 and 37°C Helicobacter pylori
0.012
-
 D-lactose mutant enzyme A128N, at pH 7.6 and 37°C Helicobacter pylori
0.033
-
 D-lactose mutant enzyme A128N/H129E, at pH 7.6 and 37°C Helicobacter pylori
0.041
-
 D-lactose mutant enzyme A128N/H129E/Y132I, at pH 7.6 and 37°C Helicobacter pylori
0.069
-
 D-lactose mutant enzyme A128N/H129E/S46F, at pH 7.6 and 37°C Helicobacter pylori
0.083
-
 D-lactose mutant enzyme A128N/H129E/Y132I/S46F, at pH 7.6 and 37°C Helicobacter pylori
0.191
-
 GDP-beta-L-fucose wild type enzyme, at pH 7.6 and 37°C Helicobacter pylori
0.308
-
 GDP-beta-L-fucose mutant enzyme A128N, at pH 7.6 and 37°C Helicobacter pylori
1.125
-
 GDP-beta-L-fucose mutant enzyme A128N/H129E, at pH 7.6 and 37°C Helicobacter pylori
1.992
-
 GDP-beta-L-fucose mutant enzyme A128N/H129E/Y132I, at pH 7.6 and 37°C Helicobacter pylori
4.742
-
 GDP-beta-L-fucose mutant enzyme A128N/H129E/S46F, at pH 7.6 and 37°C Helicobacter pylori
5.041
-
 GDP-beta-L-fucose mutant enzyme A128N/H129E/Y132I/S46F, at pH 7.6 and 37°C Helicobacter pylori