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Literature summary for 2.4.1.152 extracted from

  • Brito, C.; Gouveia, R.; Costa, J.
    Stable expression of an active soluble recombinant form of human fucosyltransferase IX in Spodoptera frugiperda Sf9 cells (2007), Biotechnol. Lett., 29, 1623-1630.
    View publication on PubMed
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Cloned(Commentary)

Cloned (Comment) Organism
full-length form of human FUT9 and a truncated form containing sFUT9 the catalytic domain of the enzyme are overexpressed in Spodoptera frugiperda (Sf9) insect cells using the signal sequence of human interleukin 2 for efficient secretion, high activity of the trunctated, soluble form sFUT9 in the supernatant of Sf9 cells, sFUT9 fucosylates sialylated and non-sialylated glycoproteins Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37800
-
 after PNGaseF treatment Homo sapiens
41300
-
 SDS-PAGE, sFUT9 Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GDP-fucose + asialo human erythropoietin
-
 Homo sapiens GDP + fucosylated asialo human erythropoietin
-
 ?
GDP-fucose + bovine asialofetuin
-
 Homo sapiens GDP + fucosylated bovine asialofetuin
-
 ?
GDP-fucose + Fucalpha1-2Galbeta1-4GlcNAc-O-(CH2)3NHCO(CH2)5-NH-biotin
-
 Homo sapiens GDP + Fucalpha1-2Galbeta1-4(Fucalpha1-3)GlcNAc-O-(CH2)3NHCO(CH2)5-biotin
-
 ?
GDP-fucose + Galbeta1-4Glc-NAc-O-(CH2)3NHCO(CH2)5-NH-biotin
-
 Homo sapiens GDP + Galbeta1-4(Fucalpha1-3)GlcNAc-O-(CH2)3NHCO(CH2)5-NH-biotin
-
 ?
GDP-fucose + Galbeta1-4GlcNAc-O-(CH2)3NHCO(CH2)5-NH-biotin sFUT9 efficiently fucosylates type II acceptors but not the corresponding sialylated acceptors, and only very poorly the type I (Galbeta3GlcNAc-R) related acceptors Homo sapiens GDP + Galbeta1-4(Fucalpha1-3)GlcNAc-O-(CH2)3NHCO(CH2)5-NH-biotin
-
 ?
GDP-fucose + human erythropoietin
-
 Homo sapiens GDP + fucosylated human erythropoietin
-
 ?

Synonyms

Synonyms Comment Organism
alpha 3-fucosyltransferase IX
-
 Homo sapiens
Fut9
-
 Homo sapiens
sFUT9 truncated form Homo sapiens