Cloned (Comment) | Organism |
---|---|
expression in Pichia pastoris GS115 cells | Apis mellifera |
expression in Sf9 cells | Apis mellifera |
the Apis mellifera genome encodes three alpha1,3-FucT homologues FucTA, FucTB and FucTC, expressed in yeast and insect cells only FucTA is found to be a core alpha1,3-FucT (EC 2.1.214), FucTC discloses to be the first Lewis-type alpha1,3-FucT (EC 2.4.1.152) to be described in insects, FucTB does not show any activity | Apis mellifera |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | reduced activity | Apis mellifera | |
additional information | not inhibited by EDTA | Apis mellifera |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | activated by Mg2+ | Apis mellifera | |
Mn2+ | activated by Mn2+ | Apis mellifera |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
47000 | - |
calculation from sequence of cDNA | Apis mellifera |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Apis mellifera | Q05GU1 | honey-bee | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Apis mellifera | - |
hypopharynx | predominantly expressed | Apis mellifera | - |
larva | - |
Apis mellifera | - |
thorax muscle | - |
Apis mellifera | - |
venom gland | predominantly expressed | Apis mellifera | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GDP-fucose + Galbeta1-4GlcNAcbeta1-2Manalpha1-6(Galbeta1-4GlcNAcbeta1-2Manalpha1-3)Manbeta1-4GlcNAcbeta1-Asn | - |
Apis mellifera | GDP + Galbeta1-4(Fucalpha1-3)GlcNAcbeta1-2Manalpha1-6(Galbeta1-4(Fucalpha1-3)GlcNAcbeta1-2Manalpha1-3)Manbeta1-4GlcNAcbeta1-Asn | - |
? | |
GDP-fucose + Galbeta1-4GlcNAcbeta1-3Galbeta1-4Glc-PA | Lacto-N-neo-tetraose is a Lewis-type FucT substrate | Apis mellifera | GDP + Galbeta1-4(Fucalpha1-3)GlcNAcbeta1-3Galbeta1-4Glc-PA | - |
? | |
GDP-fucose + GalNAcbeta1-4GlcNAcbeta1-2Manalpha1-6(GalNAcbeta1-4GlcNAcbeta1-2Manalpha1-3)Manbeta1-4GlcNAcbeta1-Asn | - |
Apis mellifera | GDP + GalNAcbeta1-4(Fucalpha1-3)GlcNAcbeta1-2Manalpha1-6(GalNAcbeta1-4(Fucalpha1-3)GlcNAcbeta1-2Manalpha1-3)Manbeta1-4GlcNAcbeta1-Asn | - |
? |
Synonyms | Comment | Organism |
---|---|---|
FucTC | - |
Apis mellifera |
LEwis-type alpha1,3-fucosyltransferase | - |
Apis mellifera |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
15 | - |
maximal activity | Apis mellifera |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | 50 | temperature range for FucTC is very broad, enzyme shows 90% of maximal activity at 4°C and 40% of maximal activity at 50°C | Apis mellifera |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
maximal activity at pH 6.5 or 8.5 | Apis mellifera |
8.5 | - |
maximal activity at pH 6.5 or 8.5 | Apis mellifera |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 9 | pH range for FucTC is very broad | Apis mellifera |