Literature summary for 2.4.1.152 extracted from

Kawar, Z.S.; Haslam, S.M.; Morris, H.R.; Dell, A.; Cummings, R.D.
Novel poly-GalNAcbeta1-4GlcNAc (LacdiNAc) and fucosylated poly-LacdiNAc N-glycans from mammalian cells expressing beta1,4-N-acetylgalactosaminyltransferase and alpha1,3-fucosyltransferase (2005), J. Biol. Chem., 280, 12810-12819.

Search for more literature for 2.4.1.152

Application

Application	Comment	Organism
synthesis	stable expression of beta1,4-N-acetylgalactosaminyltransferase from Caenorhabditis elegans and human FucT9 in CHO cells, producing fucosylated poly-LacdiNAc N-glycans	Homo sapiens

Cloned(Commentary)

Cloned (Comment)	Organism
expression in CHO cells	Homo sapiens
poly-LDN backbone is efficiently fucosylated by recombinant human alpha1,3-fucosyltransferase IX (FucT 9) when it is stably co-expressed with the Cebeta4GalNAcT in cell line L8-GalNAcT-FucT	Homo sapiens

Metals/Ions

Metals/Ions	Comment	Organism	Structure
MnCl2	glycosyltransferase assay with 20 mM MnCl2	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
GDP-fucose + (-3GalNAcbeta1-4GlcNAcbeta1-)n	-	Homo sapiens	GDP + (-3GalNAcbeta1-4(Fucalpha1-3)GlcNAcbeta1-)n	N-glycans containing poly-LDN structures are efficiently fucosylated by human FucT9 producing poly-LDNF structures, max. 4-6 repeating LDNF units	?
GDP-fucose + GalNAcbeta1-4GlcNAcbeta-R	-	Homo sapiens	GDP + GalNAcbeta1-4(Fucalpha1-3)GlcNAcbeta1-R	-	?

Synonyms

Synonyms	Comment	Organism
alpha1,3-fucosyltransferase IX	-	Homo sapiens
FucT 9	-	Homo sapiens
FucT9	-	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7	-	glycosyltransferase assay	Homo sapiens

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Release 2023.1 (January 2023)