KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0026 | - |
N-acetyllactosamine | pH 7.3, 37°C | Homo sapiens | |
0.9 | - |
NeuAcalpha(2,3)Galbeta(1,4)GlcNAc | pH 7.3, 37°C | Homo sapiens | |
1.3 | - |
Fucalpha(1,2)Galbeta(1,4)GlcNAc | - |
Homo sapiens | |
1.5 | - |
Galbeta(1,4)GlcNAcbeta(1,3)Galbeta(1,4)Glc | pH 7.3, 37°C | Homo sapiens | |
2.9 | - |
Galbeta(1,4)GlcNAc | pH 7.3, 37°C | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activates, Km: 16 mM | Homo sapiens | |
Mg2+ | activates, Km: 22 mM | Homo sapiens | |
Mn2+ | activates, Km: 12 mM | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
41000 | - |
gel filtration | Homo sapiens |
44000 | - |
1 * 44000, SDS-PAGE | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
Hep-G2 cell | - |
Homo sapiens | - |
liver | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.4 | - |
- |
Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GDP-L-fucose + alpha(2,3)-sialyllactosamine | 115% of the activity with Galbeta(1,4)GlcNAc | Homo sapiens | GDP + ? | - |
? | |
GDP-L-fucose + Fucalpha(1,2)Galbeta(1,4)GlcNAc | - |
Homo sapiens | GDP + Fucalpha(1,2)Galbeta(1,4)(Fucalpha(1,3))GlcNAc | - |
? | |
GDP-L-fucose + Galbeta(1,4)GlcNAc | i.e. N-acetyllactosamine | Homo sapiens | GDP + Galbeta(1,4)(Fucalpha(1,3))GlcNAc | - |
? | |
GDP-L-fucose + Galbeta(1,4)GlcNAcbeta(1,3)Galbeta(1,4)Glc | - |
Homo sapiens | GDP + Galbeta(1,4)(Fucalpha(1,3))GlcNAcbeta(1,3)Galbeta(1,4)Glc | - |
? | |
GDP-L-fucose + NeuAcalpha(2,3)Galbeta(1,4)GlcNAc | - |
Homo sapiens | GDP + NeuAcalpha(2,3)Galbeta(1,4)(Fucalpha(1,3))GlcNAc | - |
? | |
additional information | acceptor specificity with glycoprotein and glycolipid acceptors | Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 44000, SDS-PAGE | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 8 | reaction with N-acetyllactosamine | Homo sapiens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 8.8 | pH 6.0: about 40% of maximal activity, pH 8.8: about 50% of maximal activity, reaction with N-acetyllactosamine | Homo sapiens |