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Literature summary for 2.4.1.152 extracted from

  • Murray, B.W.; Takayama, S.; Schultz, J.; Wong, C.H.
    Mechanism and specificity of human alpha-1,3-fucosyltransferase V (1996), Biochemistry, 35, 11183-11195.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
deoxyfuconojirimycin modest inhibitor, causes 21% inhibition, synergism: combined with GDP at their Ki levels results in 80% inhibition, combined with GMP causes 50.4% inhibition Homo sapiens
GDP causes 22% inhibition Homo sapiens
GDP-alpha-D-glucose
-
Homo sapiens
GDP-alpha-D-mannose
-
Homo sapiens
GDP-fucose
-
Homo sapiens
GMP causes 23% inhibition Homo sapiens
GTP potent inhibitor Homo sapiens
Inosine diphosphate potent inhibitor Homo sapiens
L-fucal modest inhibitor, causes 26% inhibition, combined with GDP causes 40% inhibition Homo sapiens
additional information
-
Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.025
-
GDP-fucose LacNAcbeta-O-(CH2)5CO2CH3 as substrate Homo sapiens
0.06
-
GDP-fucose LacNAc as substrate Homo sapiens
0.17
-
LacNAcbeta-O-(CH2)5CO2CH3
-
Homo sapiens
1.2
-
Co2+
-
Homo sapiens
6.1
-
Mn2+
-
Homo sapiens
6.7
-
Ca2+
-
Homo sapiens
8.6
-
Mg2+
-
Homo sapiens
8.8
-
LacNAc
-
Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ alternative divalent metal cofactor Homo sapiens
Co2+ alternative divalent metal cofactor Homo sapiens
Mg2+ alternative divalent metal cofactor Homo sapiens
Mn2+ acts as electrophilic catalyst in the nonenzymatic hydrolysis of GDP-fucose Homo sapiens
Zn2+ no activity Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GDP-fucose + LacNAc
-
Homo sapiens GDP + ?
-
?
GDP-fucose + LacNAcbeta-O-(CH2)5CO2CH3
-
Homo sapiens GDP + ?
-
?
additional information catalyzes the transfer of the L-fucose moiety from guanosine diphosphate-beta-L-fucose to acceptor sugars to form biologically important fucoglycoconjugates, including sialyl Lewis x, the enzyme processes acceptor sugars with either oxygen or sulfur in the GlcNAc ring, but a similar sugar structure with nitrogen in the GlcNAc ring, is an inhibitor Homo sapiens ?
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5 7 in both phosphate and cacodylate buffers Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0062
-
GDP-fucose LacNAc as substrate Homo sapiens
0.029
-
GDP
-
Homo sapiens
0.031
-
GDP-fucose LacNAcbeta-O-(CH2)5CO2CH3 as substrate Homo sapiens
0.069
-
Inosine diphosphate
-
Homo sapiens
0.29
-
GDP-alpha-D-mannose
-
Homo sapiens
0.38
-
GDP-alpha-D-glucose
-
Homo sapiens
0.7
-
GMP
-
Homo sapiens
45
-
deoxyfuconojirimycin
-
Homo sapiens
71
-
L-fucal
-
Homo sapiens