Literature summary for 2.4.1.150 extracted from

Rao, C.V.; Janakiram, N.B.; Madka, V.; Kumar, G.; Scott, E.J.; Pathuri, G.; Bryant, T.; Kutche, H.; Zhang, Y.; Biddick, L.; Gali, H.; Zhao, Y.D.; Lightfoot, S.; Mohammed, A.
Small-molecule inhibition of GCNT3 disrupts mucin biosynthesis and malignant cellular behaviors in pancreatic cancer (2016), Cancer Res., 76, 1965-1974 .

Search for more literature for 2.4.1.150

Application

Application	Comment	Organism
pharmacology	targeting mucin biosynthesis through GCNT3 may improve drug responsiveness	Homo sapiens

Cloned(Commentary)

Cloned (Comment)	Organism
gene GCNT3, genotyping	Mus musculus

Protein Variants

Protein Variants	Comment	Organism
additional information	CRISPR-mediated knockout of GCNT3 in pancreatic cancer cells	Homo sapiens
additional information	CRISPR-mediated knockout of GCNT3 in pancreatic cancer cells. A significant increase in pancreas weight is observed in Kras mice compared with wild-type mice. Only 6% of the pancreata from Kras mice are free from PanIN lesions and PDAC, whereas 100% of pancreata are normal in wild-type mice	Mus musculus

Inhibitors

Inhibitors	Comment	Organism	Structure
talniflumate	inhibits GCNT3 and mucins in genetically engineered mice in vivo	Mus musculus

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	O95395	-	-
Mus musculus	Q5JCT0	-	-
Mus musculus C5BL/6	Q5JCT0	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
BxPC-3 cell	-	Homo sapiens	-
MiaPaCa-2 cell	-	Homo sapiens	-
PANC-1 cell	-	Homo sapiens	-
pancreas	-	Homo sapiens	-
pancreas	-	Mus musculus	-
pancreatic cancer cell	over 95% of human pancreatic cancers are associated with Kras mutations	Homo sapiens	-
pancreatic cancer cell	pancreas tumor tissue transcriptome analysis	Mus musculus	-

Synonyms

Synonyms	Comment	Organism
core 2 beta-1,6-N-acetylglucosaminyltransferase	-	Homo sapiens
core 2 beta-1,6-N-acetylglucosaminyltransferase	-	Mus musculus
gcnt3	-	Homo sapiens
gcnt3	-	Mus musculus
More	cf. EC 2.4.1.102 and 2.4.1.148	Homo sapiens
More	cf. EC 2.4.1.102 and 2.4.1.148	Mus musculus

Expression

Organism	Comment	Expression
Mus musculus	in mouse pancreatic cancer tumors, GCNT3 upregulation (103fold) is correlated with increased expression of mucins (5 to 87fold). Pancreata from 6-week-old Kras mice treated with talniflumate for 1 week show a significant decrease in GCNT3 and mucin expression in PanIN lesions. Further, GCNT3 protein expression is significantly decreased in the pancreas of talniflumate-treated Kras mice compared with untreated control mouse pancreas. mRNA expression of GCNT3 is also observed to be lower in pancreatic tissues from talniflumate-treated mice	down
Homo sapiens	talniflumate alone and in combination with low-dose gefitinib reduces GCNT3 expression, leading to the disrupted production of mucins in vivo and in vitro, in silico molecular docking studies. Talniflumate binds to GCNT3 with a docking affinity of -8.3 kcal/mol and deeper in the pocket of GCNT3. GALB1,3GALNAC's best docking affinity is -7.51 kcal/mol, achieved closer to the surface of GCNT3	down

General Information

General Information	Comment	Organism
malfunction	in mouse pancreatic cancer tumors, GCNT3 upregulation is correlated with increased expression of mucins. Aberrant GCNT3 expression is associated with increased mucin production, aggressive tumorigenesis, and reduced survival. CRISPR-mediated knockout of GCNT3 in pancreatic cancer cells reduced proliferation and spheroid formation	Mus musculus
malfunction	talniflumate alone and in combination with low-dose gefitinib reduces GCNT3 expression, leading to the disrupted production of mucins in vivo and in vitro. Aberrant GCNT3 expression is associated with increased mucin production, aggressive tumorigenesis, and reduced patient survival. CRISPR-mediated knockout of GCNT3 in pancreatic cancer cells reduced proliferation and spheroid formation	Homo sapiens
physiological function	core 2 beta-1,6-N-acetylglucosaminyltransferase (GCNT3) is a core mucin-synthesizing enzyme	Mus musculus
physiological function	core 2 beta-1,6-N-acetylglucosaminyltransferase (GCNT3) is a core mucin-synthesizing enzyme. Correlations between GCNT3 expression and pancreatic tumor progression, Kaplan-Meier analysis of patients' survival by GCNT3 expression level, overview	Homo sapiens

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Release 2023.1 (January 2023)