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Literature summary for 2.4.1.15 extracted from
- Gene cloning, expression, and characterization of trehalose-6-phosphate synthase from Pleurotus ostreatus (2017), J. Basic Microbiol., 57, 580-589 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21 cells | Pleurotus ostreatus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.14 | - |
D-glucose 6-phosphate | at pH 7.4 and 30°C | Pleurotus ostreatus |  |
| 0.17 | - |
UDP-alpha-D-glucose | at pH 7.4 and 30°C | Pleurotus ostreatus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | 12.5 mM used in assay conditions | Pleurotus ostreatus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-alpha-D-glucose + D-glucose 6-phosphate | Pleurotus ostreatus | - |
UDP + alpha,alpha-trehalose 6-phosphate | - |
? | |
| UDP-alpha-D-glucose + D-glucose 6-phosphate | Pleurotus ostreatus CCMSSC 00389 | - |
UDP + alpha,alpha-trehalose 6-phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pleurotus ostreatus | - |
- |
- |
| Pleurotus ostreatus CCMSSC 00389 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA agarose column chromatography | Pleurotus ostreatus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| mycelium | - |
Pleurotus ostreatus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-alpha-D-glucose + D-glucose 6-phosphate | - |
Pleurotus ostreatus | UDP + alpha,alpha-trehalose 6-phosphate | - |
? | |
| UDP-alpha-D-glucose + D-glucose 6-phosphate | - |
Pleurotus ostreatus CCMSSC 00389 | UDP + alpha,alpha-trehalose 6-phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 66400, SDS-PAGE | Pleurotus ostreatus |
| ? | x * 62010, calculated from amino acid sequence | Pleurotus ostreatus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| TPS1 | - |
Pleurotus ostreatus |
| trehalose-6-phosphate synthase | - |
Pleurotus ostreatus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Pleurotus ostreatus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 40 | nearly half of the activity is maintained at temperatures below 30°C, whereas most of the activities are lost at temperatures above 40°C | Pleurotus ostreatus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 5.89 | - |
D-glucose 6-phosphate | at pH 7.4 and 30°C | Pleurotus ostreatus | |
| 5.89 | - |
UDP-alpha-D-glucose | at pH 7.4 and 30°C | Pleurotus ostreatus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
- |
Pleurotus ostreatus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 8 | the enzyme is highly active at pH values from 5.0 to 8.0 | Pleurotus ostreatus |
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