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Literature summary for 2.4.1.15 extracted from
- Purification and partial biochemical-genetic characterization of trehalose 6-phosphate synthase from muscles of adult female Ascaris suum (2013), J. Helminthol., 87, 212-221.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene tps1, DNA and amino acid sequence determination and analysis, phylogenetic analysis | Ascaris suum |
| gene tps2, DNA and amino acid sequence determination and analysis, phylogenetic analysis | Ascaris suum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | 55% inhibition at 10 mM; 55% inhibition at 10 mM | Ascaris suum |  |
| Fe3+ | 32% inhibition at 10 mM; 32% inhibition at 10 mM | Ascaris suum | |
| K+ | 50% inhibition at 10 mM; 50% inhibition at 10 mM | Ascaris suum | |
| Zn2+ | - |
Ascaris suum | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| CaCl2 | activates 1.44fold at 10 mM | Ascaris suum | |
| MgCl2 | activates 2fold at 10 mM | Ascaris suum | |
| NaCl | activates at 10 mM | Ascaris suum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 66000 | - |
x * 66000, SDS-PAGE | Ascaris suum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Ascaris suum | H2BIV4 | gene tps1 | - |
| Ascaris suum | H2BIV5 | gene TPS2 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme from female worm muscle 265fold by ammonium sulfate fractionation, anion exchange chromatography, and gel filtration | Ascaris suum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| muscle | - |
Ascaris suum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 150.5 | - |
purified native enzyme, pH 4.0, 37°C | Ascaris suum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | the purified native enzyme is not absolutely substrate-specific, besides glucose 6-phosphate, the enzyme is able to use fructose 6-phosphate as an acceptor of glucose. The enzyme is inactive in the presence of glucose and fructose | Ascaris suum | ? | - |
? | |
| UDP-glucose + D-glucose 6-phosphate | - |
Ascaris suum | UDP + alpha,alpha-trehalose 6-phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 66000, SDS-PAGE | Ascaris suum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| T6P synthase | - |
Ascaris suum |
| TPS | - |
Ascaris suum |
| trehalose 6-phosphate synthase | - |
Ascaris suum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
- |
Ascaris suum |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 60 | activity range, at 40°C, the enzyme demonstrates 87.04% of its maximum activity. A rapid decrease in the enzyme activity occurs at 50°C with 35.27% of maximum activity, completely loss of activity above 60°C | Ascaris suum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 3.8 | 4.2 | - |
Ascaris suum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 7 | activity range | Ascaris suum |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Ascaris suum | isoelectric focusing | - |
5.4 |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | the enzyme belongs to the glucosyltransferase-GTB-type superfamily | Ascaris suum |
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