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Literature summary for 2.4.1.15 extracted from

  • Cardoso, F.S.; Castro, R.F.; Borges, N.; Santos, H.
    Biochemical and genetic characterization of the pathways for trehalose metabolism in Propionibacterium freudenreichii, and their role in stress response (2007), Microbiology, 153, 270-280.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
heparin activates Propionibacterium freudenreichii

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Propionibacterium freudenreichii

Inhibitors

Inhibitors Comment Organism Structure
Cu2+
-
Propionibacterium freudenreichii
NaCl an increase in the concentration of NaCl from 0 to 100 mM leads to a decrease in the OtsA activity by more than 35% when ADP-glucose or TDP-glucose is the substrate. When UDP-glucose or GDP-glucose is used as substrate, the OtsA activity is increased by 10–30% Propionibacterium freudenreichii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.3
-
UDP-glucose 37°C, pH 5.5 Propionibacterium freudenreichii
0.4
-
GDP-glucose 37°C, pH 5.5 Propionibacterium freudenreichii
0.6
-
ADP-glucose 37°C, pH 5.5 Propionibacterium freudenreichii
2
-
TDP-glucose 37°C, pH 5.5 Propionibacterium freudenreichii
3
-
D-glucose 6-phosphate 37°C, pH 5.5, cosubstrate: UDP-glucose Propionibacterium freudenreichii
4.2
-
D-glucose 6-phosphate 37°C, pH 5.5, cosubstrate: ADP-glucose Propionibacterium freudenreichii
5.3
-
D-glucose 6-phosphate 37°C, pH 5.5, cosubstrate: TDP-glucose Propionibacterium freudenreichii
7.1
-
D-glucose 6-phosphate 37°C, pH 5.5, cosubstrate: GDP-glucose Propionibacterium freudenreichii

Metals/Ions

Metals/Ions Comment Organism Structure
KCl upon an increase in the KCl concentration from 0 to 100 mM, the OtsA activity decreases by more than 40%, whereas it is not significantly affected when UDP or GDP-glucose is used as the substrate Propionibacterium freudenreichii
Mg2+ 10 mM, required for maximal activity Propionibacterium freudenreichii
NaCl an increase in the concentration of NaCl from 0 to 100 mM leads to a decrease in the OtsA activity by more than 35% when ADP-glucose or TDP-glucose is the substrate. When UDP-glucose or GDP-glucose is used as substrate, the OtsA activity is increased by 10–30% Propionibacterium freudenreichii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Propionibacterium freudenreichii the trehalose-6-phosphate synthase/phosphatase (OtsA–OtsB) pathway plays an important role in the synthesis of trehalose in response to stress ?
-
?

Organism

Organism UniProt Comment Textmining
Propionibacterium freudenreichii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Propionibacterium freudenreichii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP-alpha-D-glucose + D-glucose 6-phosphate in crude extracts of Propionibacterium freudenreichii, OtsA is specific for ADP-glucose, in contrast to the pure recombinant OtsA, which uses UDP-glucose, GDP-glucose and TDP-glucose, in addition to ADP-glucose. The substrate specificity of OtsA in cell extracts is lost during purification, and the recombinant OtsA becomes specific to ADP-glucose upon incubation with a dialysed cell extract Propionibacterium freudenreichii ADP + alpha,alpha-trehalose 6-phosphate
-
?
GDP-alpha-D-glucose + D-glucose 6-phosphate in crude extracts of Propionibacterium freudenreichii, OtsA is specific for ADP-glucose, in contrast to the pure recombinant OtsA, which uses UDP-glucose, GDP-glucose and TDP-glucose, in addition to ADP-glucose. The substrate specificity of OtsA in cell extracts is lost during purification, and the recombinant OtsA becomes specific to ADP-glucose upon incubation with a dialysed cell extract Propionibacterium freudenreichii GDP + alpha,alpha-trehalose 6-phosphate
-
?
additional information the trehalose-6-phosphate synthase/phosphatase (OtsA–OtsB) pathway plays an important role in the synthesis of trehalose in response to stress Propionibacterium freudenreichii ?
-
?
TDP-alpha-D-glucose + D-glucose 6-phosphate in crude extracts of Propionibacterium freudenreichii, OtsA is specific for ADP-glucose, in contrast to the pure recombinant OtsA, which uses UDP-glucose, GDP-glucose and TDP-glucose, in addition to ADP-glucose. The substrate specificity of OtsA in cell extracts is lost during purification, and the recombinant OtsA becomes specific to ADP-glucose upon incubation with a dialysed cell extract Propionibacterium freudenreichii TDP + alpha,alpha-trehalose 6-phosphate
-
?
UDP-alpha-D-glucose + D-glucose 6-phosphate in crude extracts of Propionibacterium freudenreichii, OtsA is specific for ADP-glucose, in contrast to the pure recombinant OtsA, which uses UDP-glucose, GDP-glucose and TDP-glucose, in addition to ADP-glucose. The substrate specificity of OtsA in cell extracts is lost during purification, and the recombinant OtsA becomes specific to ADP-glucose upon incubation with a dialysed cell extract Propionibacterium freudenreichii UDP + alpha,alpha-trehalose 6-phosphate
-
?

Synonyms

Synonyms Comment Organism
OtsA
-
Propionibacterium freudenreichii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
Propionibacterium freudenreichii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.3
-
activity with ADP-glucose, UDP-glucose and TDP-glucose Propionibacterium freudenreichii
5.5
-
activitry with GDP-glucose Propionibacterium freudenreichii

pH Range

pH Minimum pH Maximum Comment Organism
5 6 pH 5.0: about 85% of maximal activity, pH 6.0: about 50% of maximal activity Propionibacterium freudenreichii