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Literature summary for 2.4.1.15 extracted from

  • Lapp, D.; Patterson, B.W.; Elbein, A.D.
    Properties of a trehalose phosphate synthetase from Mycobacterium smegmatis (1971), J. Biol. Chem., 246, 4567-4579.
No PubMed abstract available
Search for more literature for 2.4.1.15

Activating Compound

Activating Compound Comment Organism Structure
chondroitin sulfate activation Mycolicibacterium smegmatis
heparin
-
 Mycolicibacterium smegmatis
additional information when pyrimidine sugar nucleotides are used as substrates, there is an almost absolute requirement for a high molecular weight polyanion for activity. When the purine sugar nucleotides are used as substrates fairly good enzymatic activity is observed in absence of a polyanion, this activity increases 2 to 4fold in the presence of optimum concentrations of polyanion. Activation by the polyanion is inhibited by high salt concentrations as well as by high concentrations of mononucleoside phosphates Mycolicibacterium smegmatis
Polyanions activation Mycolicibacterium smegmatis
RNA various RNA, particularly that isolated from Mycobacterium smegmatis chondroitin sulfate, activate Mycolicibacterium smegmatis

Inhibitors

Inhibitors Comment Organism Structure
GDPglucose noncompetitive to UDPglucose Mycolicibacterium smegmatis
Mycoribin
-
 Mycolicibacterium smegmatis
UDPglucose competitive to GDPglucose Mycolicibacterium smegmatis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Mycolicibacterium smegmatis
0.04
-
 UDPglucose in presence of optimal concentration of a polyanion Mycolicibacterium smegmatis
1
-
 GDPglucose in absence or presence of a polyanion Mycolicibacterium smegmatis

Metals/Ions

Metals/Ions Comment Organism Structure
Cd2+ activation Mycolicibacterium smegmatis
Co2+ activation Mycolicibacterium smegmatis
Fe2+ activation Mycolicibacterium smegmatis
K+ strongly stimulates Mycolicibacterium smegmatis
Mg2+ activation Mycolicibacterium smegmatis
Ni2+ activation Mycolicibacterium smegmatis
Zn2+ activation Mycolicibacterium smegmatis

Organism

Organism UniProt Comment Textmining
Mycolicibacterium smegmatis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Mycolicibacterium smegmatis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Mycolicibacterium smegmatis
4.8
-
 activity with UDPglucose Mycolicibacterium smegmatis
24.2
-
 activity with GDPglucose Mycolicibacterium smegmatis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP-alpha-D-glucose + D-glucose 6-phosphate
-
 Mycolicibacterium smegmatis ADP + alpha,alpha-trehalose 6-phosphate
-
 ?
CDP-glucose + D-glucose 6-phosphate
-
 Mycolicibacterium smegmatis trehalose 6-phosphate + CDP
-
 ?
dTDP-glucose + glucose 6-phosphate
-
 Mycolicibacterium smegmatis trehalose 6-phosphate + dTDP
-
 ?
GDP-glucose + D-glucose 6-phosphate
-
 Mycolicibacterium smegmatis trehalose 6-phosphate + GDP
-
 ?
UDPglucose + glucose 6-phosphate
-
 Mycolicibacterium smegmatis trehalose 6-phosphate + UDP
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Mycolicibacterium smegmatis