BRENDA - Enzyme Database show
show all sequences of 2.4.1.149

Biosynthesis in vitro of Ii core glycosphingolipids from neolactotetraosylceramide by beta1-3- and beta1-6-N-acetylglucosaminyltransferases from mouse T-lymphoma

Basu, M.; Basu, S.; J. Biol. Chem. 259, 12557-12562 (1984)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
detergent
activates, reaction rate is optimal at detergent:protein ratio of 1.6 in Triton CF-54 or X-100, 1:3 in Nonidet P-40
Mus musculus
Nonidet P-40
reaction rate is optimal at detergent:protein ratio of 1:3
Mus musculus
Triton CF-54
reaction rate is optimal at detergent:protein ratio of 1:6
Mus musculus
Triton X-100
reaction rate is optimal at detergent:protein ratio of 1:6
Mus musculus
Inhibitors
Inhibitors
Commentary
Organism
Structure
EDTA
-
Mus musculus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.09
-
neolactotetraosylceramide
-
Mus musculus
0.33
-
UDP-N-acetyl-D-glucosamine
solubilized enzyme
Mus musculus
2.5
-
UDP-N-acetyl-D-glucosamine
membrane-bound enzyme
Mus musculus
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
-
Mus musculus
16020
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Ca2+
activates, 48% as effective as Mn2+
Mus musculus
Co2+
activates, 52% as effective as Mn2+
Mus musculus
Mn2+
requirement, Km: 1.25 mM, Mn2+ cannot be replaced by Mg2+, Zn2+, Cu2+ or Cd2+
Mus musculus
additional information
not activated by Mg2+, Zn2+, Cu2+ or Cd2+
Mus musculus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Mus musculus
biosynthesis of Ii core glycosphingolipids
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Mus musculus
-
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
P-1798 cell
lymphoma cell line
Mus musculus
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
biosynthesis of Ii core glycosphingolipids
638606
Mus musculus
?
-
-
-
-
UDP-N-acetyl-D-glucosamine + asialo alpha1-acid glycoprotein
sialic acid depleted alpha1-acid glycoprotein, 30% of the activity with neolactotetraosylceramide
638606
Mus musculus
?
-
-
-
?
UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide
i.e. neolactotetraosylceramide, GlcNAcT-2, synthesis in vitro of Ii core glycosphingolipids
638606
Mus musculus
UDP + N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide
-
638606
Mus musculus
?
UDP-N-acetyl-D-glucosamine + lactosylceramide
15% of the activity with neolactotetraosylceramide
638606
Mus musculus
?
-
-
-
?
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
8
-
Mus musculus
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
detergent
activates, reaction rate is optimal at detergent:protein ratio of 1.6 in Triton CF-54 or X-100, 1:3 in Nonidet P-40
Mus musculus
Nonidet P-40
reaction rate is optimal at detergent:protein ratio of 1:3
Mus musculus
Triton CF-54
reaction rate is optimal at detergent:protein ratio of 1:6
Mus musculus
Triton X-100
reaction rate is optimal at detergent:protein ratio of 1:6
Mus musculus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
EDTA
-
Mus musculus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.09
-
neolactotetraosylceramide
-
Mus musculus
0.33
-
UDP-N-acetyl-D-glucosamine
solubilized enzyme
Mus musculus
2.5
-
UDP-N-acetyl-D-glucosamine
membrane-bound enzyme
Mus musculus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
-
Mus musculus
16020
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Ca2+
activates, 48% as effective as Mn2+
Mus musculus
Co2+
activates, 52% as effective as Mn2+
Mus musculus
Mn2+
requirement, Km: 1.25 mM, Mn2+ cannot be replaced by Mg2+, Zn2+, Cu2+ or Cd2+
Mus musculus
additional information
not activated by Mg2+, Zn2+, Cu2+ or Cd2+
Mus musculus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Mus musculus
biosynthesis of Ii core glycosphingolipids
?
-
-
-
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
P-1798 cell
lymphoma cell line
Mus musculus
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
biosynthesis of Ii core glycosphingolipids
638606
Mus musculus
?
-
-
-
-
UDP-N-acetyl-D-glucosamine + asialo alpha1-acid glycoprotein
sialic acid depleted alpha1-acid glycoprotein, 30% of the activity with neolactotetraosylceramide
638606
Mus musculus
?
-
-
-
?
UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide
i.e. neolactotetraosylceramide, GlcNAcT-2, synthesis in vitro of Ii core glycosphingolipids
638606
Mus musculus
UDP + N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide
-
638606
Mus musculus
?
UDP-N-acetyl-D-glucosamine + lactosylceramide
15% of the activity with neolactotetraosylceramide
638606
Mus musculus
?
-
-
-
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
8
-
Mus musculus
Other publictions for EC 2.4.1.149
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
722360
Peng
Helicobacter pylori beta1,3-N- ...
Helicobacter pylori
Glycobiology
22
1453-1464
2012
-
-
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-
4
-
-
-
-
-
1
-
-
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19
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4
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19
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-
-
-
-
703900
Lee
Association of beta-1,3-N-acet ...
Homo sapiens
Glycobiology
19
655-664
2009
-
-
-
-
-
-
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1
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1
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1
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2
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1
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1
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2
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706504
Bao
Tumor suppressor function of l ...
Homo sapiens
Proc. Natl. Acad. Sci. USA
106
12109-12114
2009
-
-
-
-
-
-
-
-
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1
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7
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1
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7
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1
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-
-
-
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-
2
1
1
2
-
-
689093
Biellmann
Impaired sexual behavior in ma ...
Mus musculus
Mol. Reprod. Dev.
75
699-706
2008
-
-
-
-
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-
-
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1
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2
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1
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1
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1
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-
-
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-
-
658728
Seko
beta1,3-N-Acetylglucosaminyltr ...
Homo sapiens
FEBS Lett.
556
216-220
2004
-
-
1
-
-
-
1
1
-
3
-
1
-
1
-
-
-
-
-
-
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-
5
-
1
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1
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1
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1
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1
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3
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1
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-
5
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
638581
Kataoka
A novel beta1,3-N-acetylglucos ...
Homo sapiens, Mus musculus, Mus musculus beta3GnT7
Biochem. Biophys. Res. Commun.
294
843-848
2002
-
1
2
-
-
-
-
-
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-
1
2
-
5
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-
12
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2
1
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1
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1
2
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1
2
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12
-
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2
1
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-
-
-
1
-
-
-
-
-
-
-
-
-
638583
Salo
Several polylactosamine-modify ...
Homo sapiens
Glycobiology
12
217-228
2002
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
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1
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6
-
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1
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1
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
638580
Fukuzumi
Comparison of the expression o ...
Rattus norvegicus
Glycobiology
11
481-494
2001
-
-
-
-
-
-
1
3
-
-
-
1
-
2
-
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2
1
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2
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1
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1
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3
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1
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2
1
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2
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1
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638579
McAuliffe
Synthesis of selectively radio ...
Homo sapiens
Glycoconjugate J.
16
767-772
1999
-
-
1
-
-
-
-
-
1
-
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2
-
1
-
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4
-
1
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1
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1
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2
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4
-
1
-
-
-
-
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-
-
-
638582
Sasaki
Expression cloning of cDNA enc ...
Homo sapiens
Proc. Natl. Acad. Sci. USA
94
14294-14299
1997
-
-
1
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2
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1
2
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1
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1
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5
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6
1
1
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1
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2
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1
2
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1
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5
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6
1
1
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-
638584
Hummel
Elongation of N-acetyllactosam ...
Cricetulus griseus
Eur. J. Biochem.
245
428-433
1997
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1
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1
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1
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1
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638578
Kawashima
Purification and characterizat ...
Bos taurus
J. Biol. Chem.
268
27118-27126
1993
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6
3
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3
2
5
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1
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1
1
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1
1
1
10
1
2
-
8
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1
1
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6
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3
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3
2
5
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1
1
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1
1
1
10
1
2
-
8
-
1
1
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-
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638576
Holmes
Characterization of a beta 1-- ...
Homo sapiens
Arch. Biochem. Biophys.
260
461-468
1988
6
-
-
-
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-
1
3
1
1
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1
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1
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2
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6
-
1
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2
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6
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1
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3
1
1
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1
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2
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6
-
1
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2
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-
-
638577
van den Eijnden
Biosynthesis of blood group i- ...
Rattus norvegicus
J. Biol. Chem.
263
12461-12471
1988
-
-
-
-
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6
5
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2
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2
-
1
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1
1
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1
1
1
10
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1
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1
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6
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5
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2
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2
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1
1
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1
1
1
10
-
1
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1
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638575
Hosomi
Human serum contains N-acetyll ...
Homo sapiens
J. Biochem.
95
1655-1659
1984
2
-
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3
1
2
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1
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1
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1
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5
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1
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2
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1
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638606
Basu
Biosynthesis in vitro of Ii co ...
Mus musculus
J. Biol. Chem.
259
12557-12562
1984
4
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1
3
1
4
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1
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1
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1
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4
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1
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4
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1
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3
1
4
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1
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1
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4
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1
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-
489473
Van den Eijnden
Novikoff ascites tumor cells c ...
Rattus norvegicus
J. Biol. Chem.
258
3435-3437
1983
-
-
-
-
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3
-
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3
-
1
-
1
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1
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4
-
1
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1
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3
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3
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1
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1
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4
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1
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1
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638561
Zielenski
The occurrence of two novel N- ...
Homo sapiens
FEBS Lett.
158
164-168
1983
-
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1
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1
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1
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1
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4
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1
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1
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1
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1
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1
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4
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1
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1
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