BRENDA - Enzyme Database show
show all sequences of 2.4.1.149

Expression cloning of cDNA encoding a human beta-1,3-N-acetylglucosaminyltransferase that is essential for poly-N-acetyllactosamine synthesis

Sasaki, K.; Kurata-Miura, K.; Ujita, M.; Angata, K.; Nakagawa, S.; Sekine, S.; Nishi, T.; Fukuda, M.; Proc. Natl. Acad. Sci. USA 94, 14294-14299 (1997)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
cDNA from melanoma and colonic cancer cells is cloned and overexpressed in human Burkitt lymphoma Namalwa KJM-1 cells overexpressing the i-antigen, nucleotide and amino acid sequence of the 415-amino acids protein, cDNA fragment encoding the stem region plus putative catalytic domain of iGnT fused to protein A is expressed in COS-1 cells
Homo sapiens
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
Golgi apparatus
stem region and large catalytic domain reside presumably in the Golgi lumen
Homo sapiens
5794
-
membrane
type II membrane protein topology, longest transmembrane domain among glycosyltransferases cloned so far
Homo sapiens
16020
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
47125
-
x * 47125, calculated from the amino acid sequence, mature N-glycosylated iGnT: may be approximately 53 kDa
Homo sapiens
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R
Homo sapiens
enzyme is essential for the formation of poly-N-acetyllactosamines and the i-antigen, responsible for the formation of GlcNAcbeta(1-3)Galbeta(1-4)GlcNAc-R structure and poly-N-acetyllactosamine extension
UDP + N-acetyl-beta-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R
-
-
-
UDP-N-acetyl-D-glucosamine + poly-N-acetyllactosamine
Homo sapiens
-
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Homo sapiens
O43505
-
-
Posttranslational Modification
Posttranslational Modification
Commentary
Organism
glycoprotein
2 potential N-glycosylation sites
Homo sapiens
Source Tissue
Source Tissue
Commentary
Organism
Textmining
brain
highly expressed in fetal and adult brain
Homo sapiens
-
kidney
highly expressed in fetal kidney
Homo sapiens
-
additional information
enzyme is expressed ubiquitously in various adult tissues
Homo sapiens
-
SW-1116 cell
colonic carcinoma cell line
Homo sapiens
-
WM-266-4 cell
human melanoma cell line
Homo sapiens
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
no acceptor: Galbeta(1-3)GlcNAcbeta(1-3)Galbeta(1-4)Glc
638582
Homo sapiens
?
-
-
-
-
UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R
iGnT has a unique characteristic in binding to acceptor substrates, preferentially adding poly-N-acetyllactosamine to membrane glycoproteins
638582
Homo sapiens
UDP + N-acetyl-beta-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R
-
638582
Homo sapiens
?
UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R
enzyme is essential for the formation of poly-N-acetyllactosamines and the i-antigen, responsible for the formation of GlcNAcbeta(1-3)Galbeta(1-4)GlcNAc-R structure and poly-N-acetyllactosamine extension
638582
Homo sapiens
UDP + N-acetyl-beta-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R
-
-
-
-
UDP-N-acetyl-D-glucosamine + Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glc
-
638582
Homo sapiens
UDP + GlcNAcbeta(1-3)Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glc
-
638582
Homo sapiens
?
UDP-N-acetyl-D-glucosamine + poly-N-acetyllactosamine
-
638582
Homo sapiens
?
-
-
-
-
UDP-N-acetylglucosamine + asialo-alpha1-acid glycoprotein
-
638582
Homo sapiens
UDP + N-acetylglucosaminylated asialo-alpha1-acid glycoprotein
-
638582
Homo sapiens
?
Subunits
Subunits
Commentary
Organism
?
x * 47125, calculated from the amino acid sequence, mature N-glycosylated iGnT: may be approximately 53 kDa
Homo sapiens
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Homo sapiens
Cloned(Commentary) (protein specific)
Commentary
Organism
cDNA from melanoma and colonic cancer cells is cloned and overexpressed in human Burkitt lymphoma Namalwa KJM-1 cells overexpressing the i-antigen, nucleotide and amino acid sequence of the 415-amino acids protein, cDNA fragment encoding the stem region plus putative catalytic domain of iGnT fused to protein A is expressed in COS-1 cells
Homo sapiens
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
Golgi apparatus
stem region and large catalytic domain reside presumably in the Golgi lumen
Homo sapiens
5794
-
membrane
type II membrane protein topology, longest transmembrane domain among glycosyltransferases cloned so far
Homo sapiens
16020
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
47125
-
x * 47125, calculated from the amino acid sequence, mature N-glycosylated iGnT: may be approximately 53 kDa
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R
Homo sapiens
enzyme is essential for the formation of poly-N-acetyllactosamines and the i-antigen, responsible for the formation of GlcNAcbeta(1-3)Galbeta(1-4)GlcNAc-R structure and poly-N-acetyllactosamine extension
UDP + N-acetyl-beta-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R
-
-
-
UDP-N-acetyl-D-glucosamine + poly-N-acetyllactosamine
Homo sapiens
-
?
-
-
-
Posttranslational Modification (protein specific)
Posttranslational Modification
Commentary
Organism
glycoprotein
2 potential N-glycosylation sites
Homo sapiens
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
brain
highly expressed in fetal and adult brain
Homo sapiens
-
kidney
highly expressed in fetal kidney
Homo sapiens
-
additional information
enzyme is expressed ubiquitously in various adult tissues
Homo sapiens
-
SW-1116 cell
colonic carcinoma cell line
Homo sapiens
-
WM-266-4 cell
human melanoma cell line
Homo sapiens
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
no acceptor: Galbeta(1-3)GlcNAcbeta(1-3)Galbeta(1-4)Glc
638582
Homo sapiens
?
-
-
-
-
UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R
iGnT has a unique characteristic in binding to acceptor substrates, preferentially adding poly-N-acetyllactosamine to membrane glycoproteins
638582
Homo sapiens
UDP + N-acetyl-beta-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R
-
638582
Homo sapiens
?
UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R
enzyme is essential for the formation of poly-N-acetyllactosamines and the i-antigen, responsible for the formation of GlcNAcbeta(1-3)Galbeta(1-4)GlcNAc-R structure and poly-N-acetyllactosamine extension
638582
Homo sapiens
UDP + N-acetyl-beta-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R
-
-
-
-
UDP-N-acetyl-D-glucosamine + Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glc
-
638582
Homo sapiens
UDP + GlcNAcbeta(1-3)Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glc
-
638582
Homo sapiens
?
UDP-N-acetyl-D-glucosamine + poly-N-acetyllactosamine
-
638582
Homo sapiens
?
-
-
-
-
UDP-N-acetylglucosamine + asialo-alpha1-acid glycoprotein
-
638582
Homo sapiens
UDP + N-acetylglucosaminylated asialo-alpha1-acid glycoprotein
-
638582
Homo sapiens
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 47125, calculated from the amino acid sequence, mature N-glycosylated iGnT: may be approximately 53 kDa
Homo sapiens
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Homo sapiens
Other publictions for EC 2.4.1.149
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
722360
Peng
Helicobacter pylori beta1,3-N- ...
Helicobacter pylori
Glycobiology
22
1453-1464
2012
-
-
-
-
-
-
-
4
-
-
-
-
-
1
-
-
-
-
-
-
-
-
19
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
19
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
703900
Lee
Association of beta-1,3-N-acet ...
Homo sapiens
Glycobiology
19
655-664
2009
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
706504
Bao
Tumor suppressor function of l ...
Homo sapiens
Proc. Natl. Acad. Sci. USA
106
12109-12114
2009
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
7
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
7
-
-
1
-
-
-
-
-
-
-
-
-
2
1
1
2
-
-
689093
Biellmann
Impaired sexual behavior in ma ...
Mus musculus
Mol. Reprod. Dev.
75
699-706
2008
-
-
-
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
658728
Seko
beta1,3-N-Acetylglucosaminyltr ...
Homo sapiens
FEBS Lett.
556
216-220
2004
-
-
1
-
-
-
1
1
-
3
-
1
-
1
-
-
-
-
-
-
-
-
5
-
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
1
-
3
-
1
-
-
-
-
-
-
-
-
5
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
638581
Kataoka
A novel beta1,3-N-acetylglucos ...
Homo sapiens, Mus musculus, Mus musculus beta3GnT7
Biochem. Biophys. Res. Commun.
294
843-848
2002
-
1
2
-
-
-
-
-
-
-
1
2
-
5
-
-
-
-
-
12
-
-
2
1
-
-
-
-
1
-
-
-
-
-
-
-
1
2
-
-
-
-
-
-
-
-
-
-
1
2
-
-
-
-
-
12
-
-
2
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
638583
Salo
Several polylactosamine-modify ...
Homo sapiens
Glycobiology
12
217-228
2002
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
1
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
638580
Fukuzumi
Comparison of the expression o ...
Rattus norvegicus
Glycobiology
11
481-494
2001
-
-
-
-
-
-
1
3
-
-
-
1
-
2
-
-
-
-
-
2
1
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
3
-
-
-
1
-
-
-
-
-
2
1
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
638579
McAuliffe
Synthesis of selectively radio ...
Homo sapiens
Glycoconjugate J.
16
767-772
1999
-
-
1
-
-
-
-
-
1
-
-
2
-
1
-
-
-
-
-
-
-
-
4
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
4
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
638582
Sasaki
Expression cloning of cDNA enc ...
Homo sapiens
Proc. Natl. Acad. Sci. USA
94
14294-14299
1997
-
-
1
-
-
-
-
-
2
-
1
2
-
1
-
1
-
-
-
5
-
-
6
1
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
1
2
-
-
1
-
-
5
-
-
6
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
638584
Hummel
Elongation of N-acetyllactosam ...
Cricetulus griseus
Eur. J. Biochem.
245
428-433
1997
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
638578
Kawashima
Purification and characterizat ...
Bos taurus
J. Biol. Chem.
268
27118-27126
1993
-
-
-
-
-
-
6
3
-
3
2
5
-
1
-
1
1
-
-
1
1
1
10
1
2
-
8
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
3
-
3
2
5
-
-
1
1
-
1
1
1
10
1
2
-
8
-
1
1
-
-
-
-
-
-
-
-
638576
Holmes
Characterization of a beta 1-- ...
Homo sapiens
Arch. Biochem. Biophys.
260
461-468
1988
6
-
-
-
-
-
1
3
1
1
-
1
-
1
-
-
-
-
-
2
-
-
6
-
1
-
-
-
2
-
-
-
-
-
-
6
-
-
-
-
-
-
-
1
-
3
1
1
-
1
-
-
-
-
-
2
-
-
6
-
1
-
-
-
2
-
-
-
-
-
-
-
-
-
638577
van den Eijnden
Biosynthesis of blood group i- ...
Rattus norvegicus
J. Biol. Chem.
263
12461-12471
1988
-
-
-
-
-
-
6
5
-
2
-
2
-
1
-
1
1
-
-
1
1
1
10
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
5
-
2
-
2
-
-
1
1
-
1
1
1
10
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
638575
Hosomi
Human serum contains N-acetyll ...
Homo sapiens
J. Biochem.
95
1655-1659
1984
2
-
-
-
-
-
-
3
1
2
-
1
-
1
-
-
-
-
-
1
-
-
5
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
3
1
2
-
1
-
-
-
-
-
1
-
-
5
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
638606
Basu
Biosynthesis in vitro of Ii co ...
Mus musculus
J. Biol. Chem.
259
12557-12562
1984
4
-
-
-
-
-
1
3
1
4
-
1
-
1
-
-
-
-
-
1
-
-
4
-
-
-
-
-
1
-
-
-
-
-
-
4
-
-
-
-
-
-
-
1
-
3
1
4
-
1
-
-
-
-
-
1
-
-
4
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
489473
Van den Eijnden
Novikoff ascites tumor cells c ...
Rattus norvegicus
J. Biol. Chem.
258
3435-3437
1983
-
-
-
-
-
-
3
-
-
3
-
1
-
1
-
-
-
-
-
1
-
-
4
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
3
-
1
-
-
-
-
-
1
-
-
4
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
638561
Zielenski
The occurrence of two novel N- ...
Homo sapiens
FEBS Lett.
158
164-168
1983
-
-
-
-
-
-
-
-
-
1
-
1
-
1
-
-
-
-
-
1
-
-
4
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
1
-
-
4
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-