BRENDA - Enzyme Database show
show all sequences of 2.4.1.149

Comparison of the expression of cell surface poly-N-acetyllactosamine-type oligosaccharides in PC12 cells with those in its variant PC12D

Fukuzumi, M.; Maruyama, S.; Sano, M.; Fukui, S.; Glycobiology 11, 481-494 (2001)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
additional information
nerve growth factor stimulated PC-12 cells with reduced GnT-i activity compared to unstimulated cells, no effect in PC-12D cells
Rattus norvegicus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.44
-
Galbeta(1-4)GlcNAcbeta(1-4)GlcNAc-2-aminopyridine
PC-12 cells
Rattus norvegicus
0.9
-
Galbeta(1-4)GlcNAcbeta(1-4)GlcNAc-2-aminopyridine
PC-12D cells
Rattus norvegicus
8
-
UDP-N-acetyl-D-glucosamine
PC-12 and PC-12D cells
Rattus norvegicus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
UDP-N-acetyl-D-glucosamine + poly-N-acetyllactosamine
Rattus norvegicus
biosynthesis of poly-N-acetyllactosamine chains on N-linked oligosaccharides, catalyzes a rate-limiting reaction in the expression of poly-N-acetyllactosamine chains, especially in pheochromocytoma cells
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Rattus norvegicus
-
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
PC-12 cell
pheochromacytoma cells, nerve growth factor stimulated cells with reduced GnT-i activity compared to unstimulated cells
Rattus norvegicus
-
PC-12D cell
new subline of pheochromacytoma cells PC-12, low GnT-i activity
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Rattus norvegicus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
UDP-N-acetyl-D-glucosamine + Galbeta(1-4)GlcNAcbeta(1-4)GlcNAc-2-aminopyridine
-
638580
Rattus norvegicus
UDP + GlcNAcbeta(1-3)Galbeta(1-4)GlcNAcbeta(1-4)GlcNAc-2-aminopyridine
-
638580
Rattus norvegicus
?
UDP-N-acetyl-D-glucosamine + poly-N-acetyllactosamine
biosynthesis of poly-N-acetyllactosamine chains on N-linked oligosaccharides, catalyzes a rate-limiting reaction in the expression of poly-N-acetyllactosamine chains, especially in pheochromocytoma cells
638580
Rattus norvegicus
?
-
-
-
-
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Rattus norvegicus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
additional information
nerve growth factor stimulated PC-12 cells with reduced GnT-i activity compared to unstimulated cells, no effect in PC-12D cells
Rattus norvegicus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.44
-
Galbeta(1-4)GlcNAcbeta(1-4)GlcNAc-2-aminopyridine
PC-12 cells
Rattus norvegicus
0.9
-
Galbeta(1-4)GlcNAcbeta(1-4)GlcNAc-2-aminopyridine
PC-12D cells
Rattus norvegicus
8
-
UDP-N-acetyl-D-glucosamine
PC-12 and PC-12D cells
Rattus norvegicus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
UDP-N-acetyl-D-glucosamine + poly-N-acetyllactosamine
Rattus norvegicus
biosynthesis of poly-N-acetyllactosamine chains on N-linked oligosaccharides, catalyzes a rate-limiting reaction in the expression of poly-N-acetyllactosamine chains, especially in pheochromocytoma cells
?
-
-
-
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
PC-12 cell
pheochromacytoma cells, nerve growth factor stimulated cells with reduced GnT-i activity compared to unstimulated cells
Rattus norvegicus
-
PC-12D cell
new subline of pheochromacytoma cells PC-12, low GnT-i activity
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
UDP-N-acetyl-D-glucosamine + Galbeta(1-4)GlcNAcbeta(1-4)GlcNAc-2-aminopyridine
-
638580
Rattus norvegicus
UDP + GlcNAcbeta(1-3)Galbeta(1-4)GlcNAcbeta(1-4)GlcNAc-2-aminopyridine
-
638580
Rattus norvegicus
?
UDP-N-acetyl-D-glucosamine + poly-N-acetyllactosamine
biosynthesis of poly-N-acetyllactosamine chains on N-linked oligosaccharides, catalyzes a rate-limiting reaction in the expression of poly-N-acetyllactosamine chains, especially in pheochromocytoma cells
638580
Rattus norvegicus
?
-
-
-
-
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Rattus norvegicus
Other publictions for EC 2.4.1.149
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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Peng
Helicobacter pylori beta1,3-N- ...
Helicobacter pylori
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1453-1464
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4
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1
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19
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19
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703900
Lee
Association of beta-1,3-N-acet ...
Homo sapiens
Glycobiology
19
655-664
2009
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1
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706504
Bao
Tumor suppressor function of l ...
Homo sapiens
Proc. Natl. Acad. Sci. USA
106
12109-12114
2009
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7
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1
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2
1
1
2
-
-
689093
Biellmann
Impaired sexual behavior in ma ...
Mus musculus
Mol. Reprod. Dev.
75
699-706
2008
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1
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1
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658728
Seko
beta1,3-N-Acetylglucosaminyltr ...
Homo sapiens
FEBS Lett.
556
216-220
2004
-
-
1
-
-
-
1
1
-
3
-
1
-
1
-
-
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5
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1
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1
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1
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1
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1
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3
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1
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5
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1
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1
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638581
Kataoka
A novel beta1,3-N-acetylglucos ...
Homo sapiens, Mus musculus, Mus musculus beta3GnT7
Biochem. Biophys. Res. Commun.
294
843-848
2002
-
1
2
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1
2
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5
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12
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2
1
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1
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1
2
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1
2
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12
-
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2
1
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1
-
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-
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638583
Salo
Several polylactosamine-modify ...
Homo sapiens
Glycobiology
12
217-228
2002
-
-
-
-
-
-
-
-
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1
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1
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1
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6
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1
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1
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6
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638580
Fukuzumi
Comparison of the expression o ...
Rattus norvegicus
Glycobiology
11
481-494
2001
-
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1
3
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1
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2
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2
1
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1
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1
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1
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1
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2
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1
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638579
McAuliffe
Synthesis of selectively radio ...
Homo sapiens
Glycoconjugate J.
16
767-772
1999
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-
1
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1
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2
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1
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4
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1
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1
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1
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2
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4
-
1
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-
-
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638582
Sasaki
Expression cloning of cDNA enc ...
Homo sapiens
Proc. Natl. Acad. Sci. USA
94
14294-14299
1997
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-
1
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2
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1
2
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1
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1
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5
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6
1
1
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1
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1
2
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1
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5
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6
1
1
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638584
Hummel
Elongation of N-acetyllactosam ...
Cricetulus griseus
Eur. J. Biochem.
245
428-433
1997
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638578
Kawashima
Purification and characterizat ...
Bos taurus
J. Biol. Chem.
268
27118-27126
1993
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6
3
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3
2
5
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1
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1
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1
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10
1
2
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8
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1
1
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6
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3
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1
1
10
1
2
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8
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1
1
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638576
Holmes
Characterization of a beta 1-- ...
Homo sapiens
Arch. Biochem. Biophys.
260
461-468
1988
6
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1
3
1
1
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1
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1
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6
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6
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1
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2
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638577
van den Eijnden
Biosynthesis of blood group i- ...
Rattus norvegicus
J. Biol. Chem.
263
12461-12471
1988
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6
5
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2
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2
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1
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1
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1
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10
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1
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1
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6
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1
1
10
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1
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1
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638575
Hosomi
Human serum contains N-acetyll ...
Homo sapiens
J. Biochem.
95
1655-1659
1984
2
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3
1
2
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638606
Basu
Biosynthesis in vitro of Ii co ...
Mus musculus
J. Biol. Chem.
259
12557-12562
1984
4
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489473
Van den Eijnden
Novikoff ascites tumor cells c ...
Rattus norvegicus
J. Biol. Chem.
258
3435-3437
1983
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3
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3
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638561
Zielenski
The occurrence of two novel N- ...
Homo sapiens
FEBS Lett.
158
164-168
1983
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