BRENDA - Enzyme Database show
show all sequences of 2.4.1.149

Purification and characterization of UDP-GlcNAc:Galbeta 1-4Glc(NAc)beta-1,3-N-acetylglucosaminyltransferase (poly-N-acetyllactosamine extension enzyme) from calf serum

Kawashima, H.; Yamamoto, K.; Osawa, T.; Irimura, T.; J. Biol. Chem. 268, 27118-27126 (1993)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
2-mercaptoethanol
-
Bos taurus
4-Thiouridine diphosphate
inhibits in a concentration-dependent manner
Bos taurus
CH3CN
-
Bos taurus
N-acetyllactosamine
strong product inhibition
Bos taurus
SDS
-
Bos taurus
UDP
1 mM, 70% inhibition
Bos taurus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.129
-
UDP-N-acetyl-D-glucosamine
-
Bos taurus
18.2
-
lactose
-
Bos taurus
19.6
-
N-acetyllactosamine
-
Bos taurus
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Co2+
slightly stimulates, 12.8% as effective as Mn2+ at 10 mM
Bos taurus
Mn2+
optimum concentration: 2-15 mM, cooperative activating effects by combination of Mn2+ at a low concentration and Mg2+ or Ca2+; strict requirement
Bos taurus
additional information
enzyme possesses 2 distinct metal binding sites, not activated by Ca2+, Mg2+, Zn2+, Fe2+, Cu2+, Ni2+, K+, Na+
Bos taurus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
70000
-
1 * 70000, SDS-PAGE
Bos taurus
90000
-
gel filtration
Bos taurus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
UDP-N-acetyl-D-glucosamine + glycolipid
Bos taurus
physiological acceptors: N-glycans, O-glycans, glycolipids and keratan sulfates
?
-
-
-
UDP-N-acetyl-D-glucosamine + keratan sulfate
Bos taurus
physiological acceptors: N-glycans, O-glycans, glycolipids and keratan sulfates
?
-
-
-
UDP-N-acetyl-D-glucosamine + N-glycan
Bos taurus
physiological acceptors: N-glycans, O-glycans, glycolipids and keratan sulfates
?
-
-
-
UDP-N-acetyl-D-glucosamine + O-glycan
Bos taurus
physiological acceptors: N-glycans, O-glycans, glycolipids and keratan sulfates
?
-
-
-
UDP-N-acetyl-D-glucosamine + poly-N-acetyllactosamine
Bos taurus
involved in the initiation and extension of poly-N-acetyllactosamine biosynthesis, may act as a key enzyme
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Bos taurus
-
newborn calf
-
Posttranslational Modification
Posttranslational Modification
Commentary
Organism
glycoprotein
-
Bos taurus
Purification (Commentary)
Commentary
Organism
125000fold
Bos taurus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
serum
newborn calf serum
Bos taurus
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.118
-
-
Bos taurus
Storage Stability
Storage Stability
Organism
4°C, purified enzyme, at least 2 weeks, stable
Bos taurus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
terminal Galbeta(1-4)Glc(NAc) sequences, i.e. type II chains, are preferred substrates, no substrates: terminal Galbeta(1-3)GlcNAc sequences, i.e. type I chains, Lewis X trisaccharides, i.e. Galbeta(1-4)(Fucalpha(1-3))GlcNAc, monosaccharides, e.g. galactose, asialo-bovine submaxillary mucin that contains GalNAcalpha1-Ser/Thr and Galbeta(1-3)GalNAcalpha1-Ser/Thr
638578
Bos taurus
?
-
-
-
-
UDP-N-acetyl-D-glucosamine + Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glc
i.e. lacto-N-neotetraose, 117% of activity with N-acetyllactosamine
638578
Bos taurus
UDP + GlcNAcbeta(1-3)Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glc
-
638578
Bos taurus
?
UDP-N-acetyl-D-glucosamine + glycolipid
physiological acceptors: N-glycans, O-glycans, glycolipids and keratan sulfates
638578
Bos taurus
?
-
-
-
-
UDP-N-acetyl-D-glucosamine + keratan sulfate
physiological acceptors: N-glycans, O-glycans, glycolipids and keratan sulfates
638578
Bos taurus
?
-
-
-
-
UDP-N-acetyl-D-glucosamine + N-glycan
physiological acceptors: N-glycans, O-glycans, glycolipids and keratan sulfates
638578
Bos taurus
?
-
-
-
-
UDP-N-acetyl-D-glucosamine + O-glycan
physiological acceptors: N-glycans, O-glycans, glycolipids and keratan sulfates
638578
Bos taurus
?
-
-
-
-
UDP-N-acetyl-D-glucosamine + poly-N-acetyllactosamine
involved in the initiation and extension of poly-N-acetyllactosamine biosynthesis, may act as a key enzyme
638578
Bos taurus
?
-
-
-
-
UDP-N-acetylglucosamine + asialo-alpha1-acid glycoprotein
best acceptor among the glycoproteins that contain Galbeta(1-4)GlcNAc
638578
Bos taurus
UDP + N-acetylglucosaminylated asialo-alpha1-acid glycoprotein
-
-
-
?
UDP-N-acetylglucosamine + lactose
transfer of one GlcNAc to position C-3 of the terminal Gal residue of lactose or N-acetyllactosamine in the beta linkage, 51% of activity with N-acetyllactosamine
638578
Bos taurus
UDP + GlcNAcbeta(1-3)Galbeta(1-4)Glc
-
638578
Bos taurus
?
UDP-N-acetylglucosamine + N-acetyllactosamine
transfer of one GlcNAc to position C-3 of the terminal Gal residue of lactose or N-acetyllactosamine in the beta linkage
638578
Bos taurus
UDP + GlcNAcbeta(1-3)Galbeta(1-4)GlcNAc
-
-
-
?
Subunits
Subunits
Commentary
Organism
monomer
1 * 70000, SDS-PAGE
Bos taurus
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Bos taurus
42
-
112% of activity compared with that at 37°C
Bos taurus
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
4
-
4% of activity compared with that at 37°C
Bos taurus
12
-
9% of activity compared with that at 37°C
Bos taurus
20
-
28% of activity compared with that at 37°C
Bos taurus
30
-
63% of activity compared with that at 37°C
Bos taurus
42
-
112% of activity compared with that at 37°C
Bos taurus
45
-
88% of activity compared with that at 37°C
Bos taurus
50
-
10% of activity compared with that at 37°C
Bos taurus
55
-
2% of activity compared with that at 37°C
Bos taurus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
broad pH-optimum around pH 7
Bos taurus
pH Range
pH Minimum
pH Maximum
Commentary
Organism
6
7.5
substantial reaction in the range, 65% of activity at pH 7
Bos taurus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
2-mercaptoethanol
-
Bos taurus
4-Thiouridine diphosphate
inhibits in a concentration-dependent manner
Bos taurus
CH3CN
-
Bos taurus
N-acetyllactosamine
strong product inhibition
Bos taurus
SDS
-
Bos taurus
UDP
1 mM, 70% inhibition
Bos taurus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.129
-
UDP-N-acetyl-D-glucosamine
-
Bos taurus
18.2
-
lactose
-
Bos taurus
19.6
-
N-acetyllactosamine
-
Bos taurus
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Co2+
slightly stimulates, 12.8% as effective as Mn2+ at 10 mM
Bos taurus
Mn2+
optimum concentration: 2-15 mM, cooperative activating effects by combination of Mn2+ at a low concentration and Mg2+ or Ca2+; strict requirement
Bos taurus
additional information
enzyme possesses 2 distinct metal binding sites, not activated by Ca2+, Mg2+, Zn2+, Fe2+, Cu2+, Ni2+, K+, Na+
Bos taurus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
70000
-
1 * 70000, SDS-PAGE
Bos taurus
90000
-
gel filtration
Bos taurus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
UDP-N-acetyl-D-glucosamine + glycolipid
Bos taurus
physiological acceptors: N-glycans, O-glycans, glycolipids and keratan sulfates
?
-
-
-
UDP-N-acetyl-D-glucosamine + keratan sulfate
Bos taurus
physiological acceptors: N-glycans, O-glycans, glycolipids and keratan sulfates
?
-
-
-
UDP-N-acetyl-D-glucosamine + N-glycan
Bos taurus
physiological acceptors: N-glycans, O-glycans, glycolipids and keratan sulfates
?
-
-
-
UDP-N-acetyl-D-glucosamine + O-glycan
Bos taurus
physiological acceptors: N-glycans, O-glycans, glycolipids and keratan sulfates
?
-
-
-
UDP-N-acetyl-D-glucosamine + poly-N-acetyllactosamine
Bos taurus
involved in the initiation and extension of poly-N-acetyllactosamine biosynthesis, may act as a key enzyme
?
-
-
-
Posttranslational Modification (protein specific)
Posttranslational Modification
Commentary
Organism
glycoprotein
-
Bos taurus
Purification (Commentary) (protein specific)
Commentary
Organism
125000fold
Bos taurus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
serum
newborn calf serum
Bos taurus
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.118
-
-
Bos taurus
Storage Stability (protein specific)
Storage Stability
Organism
4°C, purified enzyme, at least 2 weeks, stable
Bos taurus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
terminal Galbeta(1-4)Glc(NAc) sequences, i.e. type II chains, are preferred substrates, no substrates: terminal Galbeta(1-3)GlcNAc sequences, i.e. type I chains, Lewis X trisaccharides, i.e. Galbeta(1-4)(Fucalpha(1-3))GlcNAc, monosaccharides, e.g. galactose, asialo-bovine submaxillary mucin that contains GalNAcalpha1-Ser/Thr and Galbeta(1-3)GalNAcalpha1-Ser/Thr
638578
Bos taurus
?
-
-
-
-
UDP-N-acetyl-D-glucosamine + Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glc
i.e. lacto-N-neotetraose, 117% of activity with N-acetyllactosamine
638578
Bos taurus
UDP + GlcNAcbeta(1-3)Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glc
-
638578
Bos taurus
?
UDP-N-acetyl-D-glucosamine + glycolipid
physiological acceptors: N-glycans, O-glycans, glycolipids and keratan sulfates
638578
Bos taurus
?
-
-
-
-
UDP-N-acetyl-D-glucosamine + keratan sulfate
physiological acceptors: N-glycans, O-glycans, glycolipids and keratan sulfates
638578
Bos taurus
?
-
-
-
-
UDP-N-acetyl-D-glucosamine + N-glycan
physiological acceptors: N-glycans, O-glycans, glycolipids and keratan sulfates
638578
Bos taurus
?
-
-
-
-
UDP-N-acetyl-D-glucosamine + O-glycan
physiological acceptors: N-glycans, O-glycans, glycolipids and keratan sulfates
638578
Bos taurus
?
-
-
-
-
UDP-N-acetyl-D-glucosamine + poly-N-acetyllactosamine
involved in the initiation and extension of poly-N-acetyllactosamine biosynthesis, may act as a key enzyme
638578
Bos taurus
?
-
-
-
-
UDP-N-acetylglucosamine + asialo-alpha1-acid glycoprotein
best acceptor among the glycoproteins that contain Galbeta(1-4)GlcNAc
638578
Bos taurus
UDP + N-acetylglucosaminylated asialo-alpha1-acid glycoprotein
-
-
-
?
UDP-N-acetylglucosamine + lactose
transfer of one GlcNAc to position C-3 of the terminal Gal residue of lactose or N-acetyllactosamine in the beta linkage, 51% of activity with N-acetyllactosamine
638578
Bos taurus
UDP + GlcNAcbeta(1-3)Galbeta(1-4)Glc
-
638578
Bos taurus
?
UDP-N-acetylglucosamine + N-acetyllactosamine
transfer of one GlcNAc to position C-3 of the terminal Gal residue of lactose or N-acetyllactosamine in the beta linkage
638578
Bos taurus
UDP + GlcNAcbeta(1-3)Galbeta(1-4)GlcNAc
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 70000, SDS-PAGE
Bos taurus
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Bos taurus
42
-
112% of activity compared with that at 37°C
Bos taurus
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
4
-
4% of activity compared with that at 37°C
Bos taurus
12
-
9% of activity compared with that at 37°C
Bos taurus
20
-
28% of activity compared with that at 37°C
Bos taurus
30
-
63% of activity compared with that at 37°C
Bos taurus
42
-
112% of activity compared with that at 37°C
Bos taurus
45
-
88% of activity compared with that at 37°C
Bos taurus
50
-
10% of activity compared with that at 37°C
Bos taurus
55
-
2% of activity compared with that at 37°C
Bos taurus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
broad pH-optimum around pH 7
Bos taurus
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
6
7.5
substantial reaction in the range, 65% of activity at pH 7
Bos taurus
Other publictions for EC 2.4.1.149
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
722360
Peng
Helicobacter pylori beta1,3-N- ...
Helicobacter pylori
Glycobiology
22
1453-1464
2012
-
-
-
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-
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4
-
-
-
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1
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19
-
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4
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-
-
19
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
703900
Lee
Association of beta-1,3-N-acet ...
Homo sapiens
Glycobiology
19
655-664
2009
-
-
-
-
-
-
-
-
1
-
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1
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1
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2
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1
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1
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-
2
-
-
-
-
-
-
-
-
-
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-
-
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-
706504
Bao
Tumor suppressor function of l ...
Homo sapiens
Proc. Natl. Acad. Sci. USA
106
12109-12114
2009
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-
-
-
-
-
-
-
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1
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7
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1
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7
-
-
1
-
-
-
-
-
-
-
-
-
2
1
1
2
-
-
689093
Biellmann
Impaired sexual behavior in ma ...
Mus musculus
Mol. Reprod. Dev.
75
699-706
2008
-
-
-
-
-
-
-
-
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1
-
2
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1
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1
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1
-
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-
-
-
-
-
-
-
-
-
-
-
-
658728
Seko
beta1,3-N-Acetylglucosaminyltr ...
Homo sapiens
FEBS Lett.
556
216-220
2004
-
-
1
-
-
-
1
1
-
3
-
1
-
1
-
-
-
-
-
-
-
-
5
-
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
1
-
3
-
1
-
-
-
-
-
-
-
-
5
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
638581
Kataoka
A novel beta1,3-N-acetylglucos ...
Homo sapiens, Mus musculus, Mus musculus beta3GnT7
Biochem. Biophys. Res. Commun.
294
843-848
2002
-
1
2
-
-
-
-
-
-
-
1
2
-
5
-
-
-
-
-
12
-
-
2
1
-
-
-
-
1
-
-
-
-
-
-
-
1
2
-
-
-
-
-
-
-
-
-
-
1
2
-
-
-
-
-
12
-
-
2
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
638583
Salo
Several polylactosamine-modify ...
Homo sapiens
Glycobiology
12
217-228
2002
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
1
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
638580
Fukuzumi
Comparison of the expression o ...
Rattus norvegicus
Glycobiology
11
481-494
2001
-
-
-
-
-
-
1
3
-
-
-
1
-
2
-
-
-
-
-
2
1
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
3
-
-
-
1
-
-
-
-
-
2
1
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
638579
McAuliffe
Synthesis of selectively radio ...
Homo sapiens
Glycoconjugate J.
16
767-772
1999
-
-
1
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638582
Sasaki
Expression cloning of cDNA enc ...
Homo sapiens
Proc. Natl. Acad. Sci. USA
94
14294-14299
1997
-
-
1
-
-
-
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2
-
1
2
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1
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-
638584
Hummel
Elongation of N-acetyllactosam ...
Cricetulus griseus
Eur. J. Biochem.
245
428-433
1997
-
-
-
-
-
-
-
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-
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-
-
638578
Kawashima
Purification and characterizat ...
Bos taurus
J. Biol. Chem.
268
27118-27126
1993
-
-
-
-
-
-
6
3
-
3
2
5
-
1
-
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1
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-
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-
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-
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1
1
10
1
2
-
8
-
1
1
-
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-
-
-
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-
-
638576
Holmes
Characterization of a beta 1-- ...
Homo sapiens
Arch. Biochem. Biophys.
260
461-468
1988
6
-
-
-
-
-
1
3
1
1
-
1
-
1
-
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-
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6
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-
-
638577
van den Eijnden
Biosynthesis of blood group i- ...
Rattus norvegicus
J. Biol. Chem.
263
12461-12471
1988
-
-
-
-
-
-
6
5
-
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-
2
-
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10
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1
-
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-
-
-
-
-
-
-
638575
Hosomi
Human serum contains N-acetyll ...
Homo sapiens
J. Biochem.
95
1655-1659
1984
2
-
-
-
-
-
-
3
1
2
-
1
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-
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-
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638606
Basu
Biosynthesis in vitro of Ii co ...
Mus musculus
J. Biol. Chem.
259
12557-12562
1984
4
-
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-
-
-
1
3
1
4
-
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-
1
-
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-
-
-
489473
Van den Eijnden
Novikoff ascites tumor cells c ...
Rattus norvegicus
J. Biol. Chem.
258
3435-3437
1983
-
-
-
-
-
-
3
-
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3
-
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-
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-
-
-
-
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-
-
638561
Zielenski
The occurrence of two novel N- ...
Homo sapiens
FEBS Lett.
158
164-168
1983
-
-
-
-
-
-
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