BRENDA - Enzyme Database show
show all sequences of 2.4.1.149

Biosynthesis of blood group i-active polylactosaminoglycans. Partial purification and properties of an UDP-GlcNAc:N-acetyllactosaminide beta 1-->3-N-acetylglucosaminyltransferase from Novikoff tumor cell ascites fluid

van den Eijnden, D.H.; Koenderman, A.H.L.; Schiphorst, W.E.C.M.; J. Biol. Chem. 263, 12461-12471 (1988)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
CaCl2
weak
Rattus norvegicus
CdCl2
-
Rattus norvegicus
EDTA
strong
Rattus norvegicus
FeCl2
-
Rattus norvegicus
NiCl2
-
Rattus norvegicus
ZnCl2
strong
Rattus norvegicus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
-
Rattus norvegicus
0.6
-
asialo-alpha1-acid glycoprotein
-
Rattus norvegicus
1
-
Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)GlcNAc
-
Rattus norvegicus
2.2
-
N-acetyllactosamine
-
Rattus norvegicus
5.2
-
lactose
-
Rattus norvegicus
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mn2+
MnCl2 stimulates 2.5fold, optimal concentration: 20 mM
Rattus norvegicus
additional information
absolute requirement for divalent cations, not stimulated by Mg2+
Rattus norvegicus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R
Rattus norvegicus
enzyme functions in both the initiation and elongation of linear i-active polylactosaminoglycan chains of N-glycoproteins and possibly other glycoconjugates
UDP + N-acetyl-beta-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R
-
-
-
UDP-N-acetyl-D-glucosamine + poly-N-acetyllactosamine
Rattus norvegicus
-
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Rattus norvegicus
-
Sprague Dawley rats
-
Posttranslational Modification
Posttranslational Modification
Commentary
Organism
glycoprotein
-
Rattus norvegicus
Purification (Commentary)
Commentary
Organism
partial, 178fold, from Novikoff tumor cell ascites fluid
Rattus norvegicus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
Novikoff ascites tumor cell
strain N1S1-67
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.00153
-
-
Rattus norvegicus
Storage Stability
Storage Stability
Organism
0-4°C, lyophilized enzyme form, over 6 months, stable
Rattus norvegicus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
asialo-serum glycoproteins are much more effective than O-glycoproteins as acceptors, overview over oligosaccharide substrates
638577
Rattus norvegicus
?
-
-
-
-
UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R
highly specific for acceptor oligosaccharides and glycoproteins carrying a terminal Galbeta(1-4)GlcNAcbeta1-R unit, catalyzes the formation of GlcNAcbeta(1-3)Galbeta(1-4)GlcNAcbeta-R sequence, Galbeta(1-4)GlcNAcbeta(1-2)(Galbeta(1-4)GlcNAcbeta(1-6))Man pentasaccharide in the acceptor structure is a requirement for optimal activity, branch specificity, branches of this pentasaccharide structure, when contained in tri- and tetraantennary oligosaccharides, are highly preferred over other branches for attachment of the 1st and 2nd mol of GlcNAc into the acceptor molecule, enzyme also shows activity towards oligosaccharides related to blood group I- and i-active polylactosaminoglycans
638577
Rattus norvegicus
UDP + N-acetyl-beta-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R
-
638577
Rattus norvegicus
?
UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R
enzyme functions in both the initiation and elongation of linear i-active polylactosaminoglycan chains of N-glycoproteins and possibly other glycoconjugates
638577
Rattus norvegicus
UDP + N-acetyl-beta-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R
-
-
-
-
UDP-N-acetyl-D-glucosamine + poly-N-acetyllactosamine
-
638577
Rattus norvegicus
?
-
-
-
-
UDP-N-acetylglucosamine + asialo-alpha1-acid glycoprotein
much more effective than asialo-transferrin and asialo-fetuin, asialo-alpha1-acid glycoprotein from human plasma Cohn fraction V, transfer of GlcNAc to a terminal Gal in a beta1,3-linkage
638577
Rattus norvegicus
UDP + N-acetylglucosaminylated asialo-alpha1-acid glycoprotein
-
-
-
?
UDP-N-acetylglucosamine + asialo-fetuin
much less effective than asialo-alpha1-acid glycoprotein, transfer of GlcNAc to a terminal Gal in a beta1,3-linkage
638577
Rattus norvegicus
UDP + N-acetylglucosaminylated asialo-fetuin
-
-
-
?
UDP-N-acetylglucosamine + asialo-transferrin
much less effective than asialo-alpha1-acid glycoprotein, transfer of GlcNAc to a terminal Gal in a beta1,3-linkage
638577
Rattus norvegicus
UDP + N-acetylglucosaminylated asialo-transferrin
-
-
-
?
UDP-N-acetylglucosamine + lactose
70% of activity with N-acetyllactosamine
638577
Rattus norvegicus
UDP + GlcNAcbeta(1-3)Galbeta(1-4)Glc
-
638577
Rattus norvegicus
?
UDP-N-acetylglucosamine + N-acetyllactosamine
more effective acceptor than lactose
638577
Rattus norvegicus
UDP + GlcNAcbeta(1-3)Galbeta(1-4)GlcNAc
-
638577
Rattus norvegicus
?
UDP-N-acetylglucosamine + porcine submaxillary asialo-afuco-mucin
very poor substrate
638577
Rattus norvegicus
UDP + N-acetylglucosaminylated porcine submaxillary asialo-afuco-mucin
-
-
-
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Rattus norvegicus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.8
7.2
asialo-alpha1-acid glycoprotein as acceptor
Rattus norvegicus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
CaCl2
weak
Rattus norvegicus
CdCl2
-
Rattus norvegicus
EDTA
strong
Rattus norvegicus
FeCl2
-
Rattus norvegicus
NiCl2
-
Rattus norvegicus
ZnCl2
strong
Rattus norvegicus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
-
Rattus norvegicus
0.6
-
asialo-alpha1-acid glycoprotein
-
Rattus norvegicus
1
-
Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)GlcNAc
-
Rattus norvegicus
2.2
-
N-acetyllactosamine
-
Rattus norvegicus
5.2
-
lactose
-
Rattus norvegicus
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mn2+
MnCl2 stimulates 2.5fold, optimal concentration: 20 mM
Rattus norvegicus
additional information
absolute requirement for divalent cations, not stimulated by Mg2+
Rattus norvegicus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R
Rattus norvegicus
enzyme functions in both the initiation and elongation of linear i-active polylactosaminoglycan chains of N-glycoproteins and possibly other glycoconjugates
UDP + N-acetyl-beta-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R
-
-
-
UDP-N-acetyl-D-glucosamine + poly-N-acetyllactosamine
Rattus norvegicus
-
?
-
-
-
Posttranslational Modification (protein specific)
Posttranslational Modification
Commentary
Organism
glycoprotein
-
Rattus norvegicus
Purification (Commentary) (protein specific)
Commentary
Organism
partial, 178fold, from Novikoff tumor cell ascites fluid
Rattus norvegicus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
Novikoff ascites tumor cell
strain N1S1-67
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.00153
-
-
Rattus norvegicus
Storage Stability (protein specific)
Storage Stability
Organism
0-4°C, lyophilized enzyme form, over 6 months, stable
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
asialo-serum glycoproteins are much more effective than O-glycoproteins as acceptors, overview over oligosaccharide substrates
638577
Rattus norvegicus
?
-
-
-
-
UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R
highly specific for acceptor oligosaccharides and glycoproteins carrying a terminal Galbeta(1-4)GlcNAcbeta1-R unit, catalyzes the formation of GlcNAcbeta(1-3)Galbeta(1-4)GlcNAcbeta-R sequence, Galbeta(1-4)GlcNAcbeta(1-2)(Galbeta(1-4)GlcNAcbeta(1-6))Man pentasaccharide in the acceptor structure is a requirement for optimal activity, branch specificity, branches of this pentasaccharide structure, when contained in tri- and tetraantennary oligosaccharides, are highly preferred over other branches for attachment of the 1st and 2nd mol of GlcNAc into the acceptor molecule, enzyme also shows activity towards oligosaccharides related to blood group I- and i-active polylactosaminoglycans
638577
Rattus norvegicus
UDP + N-acetyl-beta-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R
-
638577
Rattus norvegicus
?
UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R
enzyme functions in both the initiation and elongation of linear i-active polylactosaminoglycan chains of N-glycoproteins and possibly other glycoconjugates
638577
Rattus norvegicus
UDP + N-acetyl-beta-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R
-
-
-
-
UDP-N-acetyl-D-glucosamine + poly-N-acetyllactosamine
-
638577
Rattus norvegicus
?
-
-
-
-
UDP-N-acetylglucosamine + asialo-alpha1-acid glycoprotein
much more effective than asialo-transferrin and asialo-fetuin, asialo-alpha1-acid glycoprotein from human plasma Cohn fraction V, transfer of GlcNAc to a terminal Gal in a beta1,3-linkage
638577
Rattus norvegicus
UDP + N-acetylglucosaminylated asialo-alpha1-acid glycoprotein
-
-
-
?
UDP-N-acetylglucosamine + asialo-fetuin
much less effective than asialo-alpha1-acid glycoprotein, transfer of GlcNAc to a terminal Gal in a beta1,3-linkage
638577
Rattus norvegicus
UDP + N-acetylglucosaminylated asialo-fetuin
-
-
-
?
UDP-N-acetylglucosamine + asialo-transferrin
much less effective than asialo-alpha1-acid glycoprotein, transfer of GlcNAc to a terminal Gal in a beta1,3-linkage
638577
Rattus norvegicus
UDP + N-acetylglucosaminylated asialo-transferrin
-
-
-
?
UDP-N-acetylglucosamine + lactose
70% of activity with N-acetyllactosamine
638577
Rattus norvegicus
UDP + GlcNAcbeta(1-3)Galbeta(1-4)Glc
-
638577
Rattus norvegicus
?
UDP-N-acetylglucosamine + N-acetyllactosamine
more effective acceptor than lactose
638577
Rattus norvegicus
UDP + GlcNAcbeta(1-3)Galbeta(1-4)GlcNAc
-
638577
Rattus norvegicus
?
UDP-N-acetylglucosamine + porcine submaxillary asialo-afuco-mucin
very poor substrate
638577
Rattus norvegicus
UDP + N-acetylglucosaminylated porcine submaxillary asialo-afuco-mucin
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Rattus norvegicus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.8
7.2
asialo-alpha1-acid glycoprotein as acceptor
Rattus norvegicus
Other publictions for EC 2.4.1.149
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
722360
Peng
Helicobacter pylori beta1,3-N- ...
Helicobacter pylori
Glycobiology
22
1453-1464
2012
-
-
-
-
-
-
-
4
-
-
-
-
-
1
-
-
-
-
-
-
-
-
19
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
19
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
703900
Lee
Association of beta-1,3-N-acet ...
Homo sapiens
Glycobiology
19
655-664
2009
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
706504
Bao
Tumor suppressor function of l ...
Homo sapiens
Proc. Natl. Acad. Sci. USA
106
12109-12114
2009
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
7
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
7
-
-
1
-
-
-
-
-
-
-
-
-
2
1
1
2
-
-
689093
Biellmann
Impaired sexual behavior in ma ...
Mus musculus
Mol. Reprod. Dev.
75
699-706
2008
-
-
-
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
658728
Seko
beta1,3-N-Acetylglucosaminyltr ...
Homo sapiens
FEBS Lett.
556
216-220
2004
-
-
1
-
-
-
1
1
-
3
-
1
-
1
-
-
-
-
-
-
-
-
5
-
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
1
-
3
-
1
-
-
-
-
-
-
-
-
5
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
638581
Kataoka
A novel beta1,3-N-acetylglucos ...
Homo sapiens, Mus musculus, Mus musculus beta3GnT7
Biochem. Biophys. Res. Commun.
294
843-848
2002
-
1
2
-
-
-
-
-
-
-
1
2
-
5
-
-
-
-
-
12
-
-
2
1
-
-
-
-
1
-
-
-
-
-
-
-
1
2
-
-
-
-
-
-
-
-
-
-
1
2
-
-
-
-
-
12
-
-
2
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
638583
Salo
Several polylactosamine-modify ...
Homo sapiens
Glycobiology
12
217-228
2002
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
1
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
638580
Fukuzumi
Comparison of the expression o ...
Rattus norvegicus
Glycobiology
11
481-494
2001
-
-
-
-
-
-
1
3
-
-
-
1
-
2
-
-
-
-
-
2
1
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
3
-
-
-
1
-
-
-
-
-
2
1
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
638579
McAuliffe
Synthesis of selectively radio ...
Homo sapiens
Glycoconjugate J.
16
767-772
1999
-
-
1
-
-
-
-
-
1
-
-
2
-
1
-
-
-
-
-
-
-
-
4
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
4
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
638582
Sasaki
Expression cloning of cDNA enc ...
Homo sapiens
Proc. Natl. Acad. Sci. USA
94
14294-14299
1997
-
-
1
-
-
-
-
-
2
-
1
2
-
1
-
1
-
-
-
5
-
-
6
1
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
1
2
-
-
1
-
-
5
-
-
6
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
638584
Hummel
Elongation of N-acetyllactosam ...
Cricetulus griseus
Eur. J. Biochem.
245
428-433
1997
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
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1
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