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Literature summary for 2.4.1.149 extracted from

  • van den Eijnden, D.H.; Koenderman, A.H.L.; Schiphorst, W.E.C.M.
    Biosynthesis of blood group i-active polylactosaminoglycans. Partial purification and properties of an UDP-GlcNAc:N-acetyllactosaminide beta 1-->3-N-acetylglucosaminyltransferase from Novikoff tumor cell ascites fluid (1988), J. Biol. Chem., 263, 12461-12471.
    View publication on PubMed
Search for more literature for 2.4.1.149

Inhibitors

Inhibitors Comment Organism Structure
CaCl2 weak Rattus norvegicus
CdCl2
-
 Rattus norvegicus
EDTA strong Rattus norvegicus
FeCl2
-
 Rattus norvegicus
NiCl2
-
 Rattus norvegicus
ZnCl2 strong Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Rattus norvegicus
0.6
-
 asialo-alpha1-acid glycoprotein
-
 Rattus norvegicus
1
-
 Galbeta(1->4)GlcNAcbeta(1->3)Galbeta(1->4)GlcNAc
-
 Rattus norvegicus
2.2
-
 N-acetyllactosamine
-
 Rattus norvegicus
5.2
-
 lactose
-
 Rattus norvegicus

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ MnCl2 stimulates 2.5fold, optimal concentration: 20 mM Rattus norvegicus
additional information absolute requirement for divalent cations, not stimulated by Mg2+ Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R Rattus norvegicus enzyme functions in both the initiation and elongation of linear i-active polylactosaminoglycan chains of N-glycoproteins and possibly other glycoconjugates UDP + N-acetyl-beta-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R
-
 ?
UDP-N-acetyl-D-glucosamine + poly-N-acetyllactosamine Rattus norvegicus
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
 Sprague Dawley rats
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Rattus norvegicus

Purification (Commentary)

Purification (Comment) Organism
partial, 178fold, from Novikoff tumor cell ascites fluid Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
Novikoff ascites tumor cell strain N1S1-67 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00153
-
-
 Rattus norvegicus

Storage Stability

Storage Stability Organism
0-4°C, lyophilized enzyme form, over 6 months, stable Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information asialo-serum glycoproteins are much more effective than O-glycoproteins as acceptors, overview over oligosaccharide substrates Rattus norvegicus ?
-
 ?
UDP-N-acetyl-alpha-D-glucosamine + Galbeta(1->4)GlcNAcbeta(1->3)Galbeta(1->4)GlcNAc
-
 Rattus norvegicus UDP + GlcNAcbeta(1->3)Galbeta(1->4)GlcNAcbeta(1->3)Galbeta(1->4)GlcNAc
-
 ?
UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R highly specific for acceptor oligosaccharides and glycoproteins carrying a terminal Galbeta(1-4)GlcNAcbeta1-R unit, catalyzes the formation of GlcNAcbeta(1-3)Galbeta(1-4)GlcNAcbeta-R sequence, Galbeta(1-4)GlcNAcbeta(1-2)(Galbeta(1-4)GlcNAcbeta(1-6))Man pentasaccharide in the acceptor structure is a requirement for optimal activity, branch specificity, branches of this pentasaccharide structure, when contained in tri- and tetraantennary oligosaccharides, are highly preferred over other branches for attachment of the 1st and 2nd mol of GlcNAc into the acceptor molecule, enzyme also shows activity towards oligosaccharides related to blood group I- and i-active polylactosaminoglycans Rattus norvegicus UDP + N-acetyl-beta-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R
-
 ?
UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R enzyme functions in both the initiation and elongation of linear i-active polylactosaminoglycan chains of N-glycoproteins and possibly other glycoconjugates Rattus norvegicus UDP + N-acetyl-beta-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R
-
 ?
UDP-N-acetyl-D-glucosamine + poly-N-acetyllactosamine
-
 Rattus norvegicus ?
-
 ?
UDP-N-acetylglucosamine + asialo-alpha1-acid glycoprotein much more effective than asialo-transferrin and asialo-fetuin, asialo-alpha1-acid glycoprotein from human plasma Cohn fraction V, transfer of GlcNAc to a terminal Gal in a beta1,3-linkage Rattus norvegicus UDP + N-acetylglucosaminylated asialo-alpha1-acid glycoprotein
-
 ?
UDP-N-acetylglucosamine + asialo-fetuin much less effective than asialo-alpha1-acid glycoprotein, transfer of GlcNAc to a terminal Gal in a beta1,3-linkage Rattus norvegicus UDP + N-acetylglucosaminylated asialo-fetuin
-
 ?
UDP-N-acetylglucosamine + asialo-transferrin much less effective than asialo-alpha1-acid glycoprotein, transfer of GlcNAc to a terminal Gal in a beta1,3-linkage Rattus norvegicus UDP + N-acetylglucosaminylated asialo-transferrin
-
 ?
UDP-N-acetylglucosamine + lactose 70% of activity with N-acetyllactosamine Rattus norvegicus UDP + GlcNAcbeta(1-3)Galbeta(1-4)Glc
-
 ?
UDP-N-acetylglucosamine + N-acetyllactosamine more effective acceptor than lactose Rattus norvegicus UDP + GlcNAcbeta(1-3)Galbeta(1-4)GlcNAc
-
 ?
UDP-N-acetylglucosamine + porcine submaxillary asialo-afuco-mucin very poor substrate Rattus norvegicus UDP + N-acetylglucosaminylated porcine submaxillary asialo-afuco-mucin
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8 7.2 asialo-alpha1-acid glycoprotein as acceptor Rattus norvegicus