Literature summary for 2.4.1.145 extracted from

Nishino, K.; Yamamoto, E.; Niimi, K.; Sekiya, Y.; Yamashita, Y.; Kikkawa, F.
N-acetylglucosaminyltransferase IVa promotes invasion of choriocarcinoma (2017), Oncol. Rep., 38, 440-448 .

Search for more literature for 2.4.1.145

Protein Variants

Protein Variants	Comment	Organism
additional information	establishment of a choriocarcinoma cell line with GnT-IVa overexpression (Jar-GnT4a), and examination of its malignant potential and target proteins for GnT-IVa glycosylation. GnT-IVa overexpression increases the cell migration and invasion (2.5 and 1.4fold) as well as the ability to adhere to the extracellular matrix (ECM) components, including fibronectin and collagen type I and IV. The tumour formation potential of Jar-GnT4a in mice is significantly higher than that of control, and the cumulative survival rate of mice with Jar-GnT4a is relatively lower than those with control. Immunoprecipitation studies show that beta1,4GlcNAc branches of N-glycans on integrin beta1 in choriocarcinoma cells are increased by GnT-IVa overexpression	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
UDP-N-acetyl-alpha-D-glucosamine + beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn-[lysosome-associated membrane glycoprotein 2]	Homo sapiens	i.e. LAMP-2, LAMP-2 has 16 N-glycans which play crucial roles in cell adhesion to ECM and metastasis of cancer cells because N-glycans configure cell surface ligands of cancer cells for galectin-1, galectin-3, and selectins on extracellular matrix or the membranes of the counter cell	UDP + beta-D-GlcNAc-(1->2)-[beta-D-GlcNAc-(1->4)]-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn-[lysosome-associated membrane glycoprotein 2]	-	?
UDP-N-acetyl-alpha-D-glucosamine + beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn-[protein]	Homo sapiens	-	UDP + beta-D-GlcNAc-(1->2)-[beta-D-GlcNAc-(1->4)]-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn-[protein]	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	Q9UM21	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
choriocarcinoma cell	-	Homo sapiens	-
Jar-GnT4a cell	-	Homo sapiens	-
trophoblast	-	Homo sapiens	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
UDP-N-acetyl-alpha-D-glucosamine + beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn-[integrin beta1]	-	Homo sapiens	UDP + beta-D-GlcNAc-(1->2)-[beta-D-GlcNAc-(1->4)]-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn-[integrin beta1]	-	?
UDP-N-acetyl-alpha-D-glucosamine + beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn-[lysosome-associated membrane glycoprotein 2]	i.e. LAMP-2, LAMP-2 has 16 N-glycans which play crucial roles in cell adhesion to ECM and metastasis of cancer cells because N-glycans configure cell surface ligands of cancer cells for galectin-1, galectin-3, and selectins on extracellular matrix or the membranes of the counter cell	Homo sapiens	UDP + beta-D-GlcNAc-(1->2)-[beta-D-GlcNAc-(1->4)]-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn-[lysosome-associated membrane glycoprotein 2]	-	?
UDP-N-acetyl-alpha-D-glucosamine + beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn-[lysosome-associated membrane glycoprotein 2]	i.e. LAMP-2	Homo sapiens	UDP + beta-D-GlcNAc-(1->2)-[beta-D-GlcNAc-(1->4)]-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn-[lysosome-associated membrane glycoprotein 2]	-	?
UDP-N-acetyl-alpha-D-glucosamine + beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn-[protein]	-	Homo sapiens	UDP + beta-D-GlcNAc-(1->2)-[beta-D-GlcNAc-(1->4)]-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn-[protein]	-	?

Synonyms

Synonyms	Comment	Organism
GnT-IV	-	Homo sapiens
GnT-IVa	-	Homo sapiens
Mgat4a	-	Homo sapiens
N-acetylglucosaminyltransferase IV	-	Homo sapiens

General Information

General Information	Comment	Organism
malfunction	GnT-IVa overexpression increases cell adhesion, migration and invasion abilities of Jar cells. GnT-IVa overexpression increases cellular interaction with ECM as demonstrated by the relative adhesion rates of mock cells and Jar-GnT4a cells on fibronectin, collagen type I and collagen type IV. GnT-IVa knockdown in choriocarcinoma cells suppresses migration and invasion and decreases cellular adhesion to extracellular matrix	Homo sapiens
physiological function	N-acetylglucosaminyltransferase IV (GnT-IV) is a glycosyltransferase which catalyses the formation of beta1,4GlcNAc branches on the mannose core of N-glycans. beta1,4GlcNAc branches on human chorionic gonadotropin (hCG) are detected in GTN but not in normal pregnancy or hydatidiform mole. N-acetylglucosaminyltransferase IVa promotes invasion of choriocarcinoma. Identification of target proteins for GnT-IVa glycosylation which contribute to the malignancy of choriocarcinoma, overview. GnT-IVa overexpression increases highly branched N-glycans on integrin beta1. Highly branched N-glycans resulting from the action of GnT-IVa are strongly detected in invasive mole and choriocarcinoma, in proportion to the GnT-IVa protein expression. GnT-IVa may play an important role in accelerating the malignancy of choriocarcinoma through addition of beta1,4GlcNAc branches to the N-glycans on some proteins	Homo sapiens

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Release 2023.1 (January 2023)