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Literature summary for 2.4.1.144 extracted from

  • Ikeda, Y.; Koyota, S.; Ihara, H.; Yamaguchi, Y.; Korekane, H.; Tsuda, T.; Sasai, K.; Taniguchi, N.
    Kinetic basis for the donor nucleotide-sugar specificity of beta1,4-N-acetylglucosaminyltransferase III (2000), J. Biochem., 128, 609-619.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of soluble His-tagged protein in Spodoptera frugiperda Sf 21 insect cells via baculovirus infection, secretion into the medium Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
ADP
-
Rattus norvegicus
ADP-glucose competitive against UDP-N-acetyl-D-glucosamine Rattus norvegicus
alpha-N-acetyl-D-glucosamine-1-phosphate
-
Rattus norvegicus
CDP
-
Rattus norvegicus
CDP-glucose competitive against UDP-N-acetyl-D-glucosamine Rattus norvegicus
GDP
-
Rattus norvegicus
GDP-glucose competitive against UDP-N-acetyl-D-glucosamine Rattus norvegicus
N-acetyl-D-glucosamine
-
Rattus norvegicus
TDP
-
Rattus norvegicus
TDP-glucose competitive against UDP-N-acetyl-D-glucosamine Rattus norvegicus
UDP
-
Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.021
-
pyridylaminated acceptor substrate recombinant enzyme Rattus norvegicus
0.021
-
pyridylaminated acceptor substrate with UDP-GlcNAc as donor substrate Rattus norvegicus
0.023
-
pyridylaminated acceptor substrate recombinant enzyme Rattus norvegicus
0.023
-
pyridylaminated acceptor substrate with UDP-Glc as donor substrate Rattus norvegicus
0.024
-
pyridylaminated acceptor substrate recombinant enzyme Rattus norvegicus
0.024
-
pyridylaminated acceptor substrate with UDP-GalNAc as donor substrate Rattus norvegicus
0.42
-
UDP-N-acetyl-D-glucosamine recombinant enzyme Rattus norvegicus
1
-
UDP-D-glucose recombinant enzyme Rattus norvegicus
3.6
-
UDP-N-acetyl-D-galactosamine recombinant enzyme Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
x * 60000 + x * 50000, SDS-PAGE Rattus norvegicus
60000
-
x * 60000 + x * 50000, SDS-PAGE Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged protein from medium of Spodoptera frugiperda Sf 21 cell culture Rattus norvegicus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.02
-
purified recombinant enzyme Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information UDP-galactose is no substrate Rattus norvegicus ?
-
?
additional information ADP-, CDP-, GDP-, and TDP-glucose are no donor substrates Rattus norvegicus ?
-
?
UDP-D-glucose + (N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6)-beta-D-mannosyl-1,4-N-acetyl-beta-D-glucosaminyl-R pyridylaminated biantennary sugar chain Rattus norvegicus UDP + N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6)-(beta-D-glucosyl-1,4)-beta-D-mannosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
-
?
UDP-D-glucose + (N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6)-beta-D-mannosyl-1,4-N-acetyl-beta-D-glucosaminyl-R 0.2% activity compared to donor substrate UDP-N-acetyl-D-glucosamine Rattus norvegicus UDP + N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6)-(beta-D-glucosyl-1,4)-beta-D-mannosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
-
?
UDP-N-acetyl-D-galactosamine + (N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6)-beta-D-mannosyl-1,4-N-acetyl-beta-D-glucosaminyl-R pyridylaminated biantennary sugar chain Rattus norvegicus UDP + N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6)-(N-acetyl-beta-D-galactosaminyl-1,4)-beta-D-mannosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
-
?
UDP-N-acetyl-D-galactosamine + (N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6)-beta-D-mannosyl-1,4-N-acetyl-beta-D-glucosaminyl-R 0.1% activity compared to donor substrate UDP-N-acetyl-D-glucosamine Rattus norvegicus UDP + N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6)-(N-acetyl-beta-D-galactosaminyl-1,4)-beta-D-mannosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
-
?
UDP-N-acetyl-D-glucosamine + (N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6)-beta-D-mannosyl-1,4-N-acetyl-beta-D-glucosaminyl-R pyridylaminated biantennary sugar chain Rattus norvegicus UDP + N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6)-(N-acetyl-beta-D-glucosaminyl-1,4)-beta-D-mannosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
-
?
UDP-N-acetyl-D-glucosamine + pyridylaminated acceptor substrate
-
Rattus norvegicus ?
-
?

Subunits

Subunits Comment Organism
? x * 60000 + x * 50000, SDS-PAGE Rattus norvegicus

Synonyms

Synonyms Comment Organism
beta1,4-N-acetylglucosaminyltransferase III
-
Rattus norvegicus
GnT-III
-
Rattus norvegicus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.75
-
ADP-glucose recombinant enzyme Rattus norvegicus
1
-
GDP-glucose recombinant enzyme Rattus norvegicus
3.9
-
CDP-glucose recombinant enzyme Rattus norvegicus