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Literature summary for 2.4.1.140 extracted from

  • Wangpaiboon, K.; Padungros, P.; Nakapong, S.; Charoenwongpaiboon, T.; Rejzek, M.; Field, R.A.; Pichyangkura, R.
    An alpha-1,6-and alpha-1,3-linked glucan produced by Leuconostoc citreum ABK-1 alternansucrase with nanoparticle and film-forming properties (2018), Sci. Rep., 8, 8340 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.1.140

Activating Compound

Activating Compound Comment Organism Structure
additional information no effect by 10 mM EDTA Leuconostoc citreum
Triton X-100 21% activation at 0.1% v/v Leuconostoc citreum

Cloned(Commentary)

Cloned (Comment) Organism
gene Lcalt, rcombinant expression in Escherichia coli strain BL21(DE3) Leuconostoc citreum

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 86% inhibition at 10 mM Leuconostoc citreum
Fe3+ 94% inhibition at 10 mM Leuconostoc citreum
additional information no effect by 10 mM EDTA Leuconostoc citreum
Zn2+ 39% inhibition at 10 mM Leuconostoc citreum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
32.2
-
 sucrose pH 5.0, 40°C, recombinant enzyme Leuconostoc citreum

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 22% activation at 10 mM Leuconostoc citreum
Co2+ 10% activation at 10 mM Leuconostoc citreum
Mn2+ activates 2.4fold, 138% Leuconostoc citreum
additional information no effect by 10 mM Mg2+ Leuconostoc citreum

Organism

Organism UniProt Comment Textmining
Leuconostoc citreum D3JC16
-
-
Leuconostoc citreum ABK-1 D3JC16
-
-

Purification (Commentary)

Purification (Comment) Organism
rcombinant enzyme from Escherichia coli strain BL21(DE3) by anion exchange chromatography, hydrophobic interaction chromatography, and dialysis Leuconostoc citreum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
34.7
-
 purified recombinant enzyme, pH 5.0, 40°C Leuconostoc citreum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 sucrose
-
 Leuconostoc citreum ?
-
 ?
2 sucrose
-
 Leuconostoc citreum ABK-1 ?
-
 ?
additional information product analysis, determination of alternan nanoparticle size distribution by dynamic light scattering Leuconostoc citreum ?
-
-
additional information product analysis, determination of alternan nanoparticle size distribution by dynamic light scattering Leuconostoc citreum ABK-1 ?
-
-
sucrose + alpha-D-glucan
-
 Leuconostoc citreum ?
-
 ?
sucrose + alpha-D-glucan
-
 Leuconostoc citreum ABK-1 ?
-
 ?

Synonyms

Synonyms Comment Organism
alternansucrase
-
 Leuconostoc citreum
Lcalt
-
 Leuconostoc citreum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
 recombinant enzyme Leuconostoc citreum

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 55 activity range, profile overview Leuconostoc citreum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
290
-
 sucrose pH 5.0, 40°C, recombinant enzyme Leuconostoc citreum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
 recombinant enzyme Leuconostoc citreum

General Information

General Information Comment Organism
evolution the enzyme belongs to the CAZy glycoside hydrolase family 70 (GH70) Leuconostoc citreum
physiological function alternansucrase catalyses the sequential transfer of glucose residues from sucrose onto another sucrose molecule to form a long chain polymer, known as alternan. The insoluble polymer alternan has alternating alpha-1,6 and alpha-1,3 glycosidic linkages as has been judged by using 13C NMR spectroscopy and methylation analysis, partially hydrolysed product analysis, and enzymatic analysis Leuconostoc citreum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
9
-
 sucrose pH 5.0, 40°C, recombinant enzyme Leuconostoc citreum