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Literature summary for 2.4.1.140 extracted from

  • Cote, G.L.; Dunlap, C.A.; Vermillion, K.E.
    Glucosylation of raffinose via alternansucrase acceptor reactions (2009), Carbohydr. Res., 344, 1951-1959.
    View publication on PubMed

Application

Application Comment Organism
drug development formation of oligosaccharides by alternansucrase may serve as prebiotics Leuconostoc mesenteroides

Organism

Organism UniProt Comment Textmining
Leuconostoc mesenteroides
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NRRL B-21297
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Leuconostoc mesenteroides NRRL B-21297
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NRRL B-21297
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sucrose + raffinose the alternansucrase-catalyzed acceptor reaction with raffinose gives a gravimetric yield of alternan of 9.5% relative to the weight of sucrose, indicating that 20% of the glucosyl units are incorporated into alternan, and 80% into oligosaccharide acceptor products. The main products are the tetrasaccharides alpha-D-Glcp-(1-3)-alpha-D-Galp-(1-6)-alpha-D-Glcp-(1-2)-beta-D-Fruf and alpha-D-Glcp-(1-4)-alpha-D-Galp-(1-6)-alpha-D-Glcp-(1-2)-beta-D-Fruf in ratios ranging from 4:1 to 9:1, along with lesser amounts of alpha-D-Glcp-(1-6)-alpha-D-Galp-(1-6)-alpha-D-Glcp-(1-2)-beta-D-Fruf. Pentasaccharides, hexasaccharides and higher oligosaccharides are also produced Leuconostoc mesenteroides ?
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?
sucrose + raffinose the alternansucrase-catalyzed acceptor reaction with raffinose gives a gravimetric yield of alternan of 9.5% relative to the weight of sucrose, indicating that 20% of the glucosyl units are incorporated into alternan, and 80% into oligosaccharide acceptor products. The main products are the tetrasaccharides alpha-D-Glcp-(1-3)-alpha-D-Galp-(1-6)-alpha-D-Glcp-(1-2)-beta-D-Fruf and alpha-D-Glcp-(1-4)-alpha-D-Galp-(1-6)-alpha-D-Glcp-(1-2)-beta-D-Fruf in ratios ranging from 4:1 to 9:1, along with lesser amounts of alpha-D-Glcp-(1-6)-alpha-D-Galp-(1-6)-alpha-D-Glcp-(1-2)-beta-D-Fruf. Pentasaccharides, hexasaccharides and higher oligosaccharides are also produced Leuconostoc mesenteroides NRRL B-21297 ?
-
?

Synonyms

Synonyms Comment Organism
alternansucrase
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Leuconostoc mesenteroides
glucansucrase
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Leuconostoc mesenteroides