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Literature summary for 2.4.1.14 extracted from

  • Sawitri, W.D.; Afidah, S.N.; Nakagawa, A.; Hase, T.; Sugiharto, B.
    Identification of UDP-glucose binding site in glycosyltransferase domain of sucrose phosphate synthase from sugarcane (Saccharum officinarum) by structure-based site-directed mutagenesis (2018), Biophys. Rev., 10, 293-298 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.1.14

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Saccharum officinarum

Protein Variants

Protein Variants Comment Organism
D498A the mutant shows strongly decreased activity compared to the wild type enzyme Saccharum officinarum
P497A the mutant shows slightly increased activity compared to the wild type enzyme Saccharum officinarum
R496A the mutation leads to loss of enzyme activity Saccharum officinarum
S495A the mutant shows about 2fold increased activity compared to the wild type enzyme Saccharum officinarum
V570A the mutation leads to loss of enzyme activity Saccharum officinarum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-alpha-D-glucose + D-fructose 6-phosphate Saccharum officinarum
-
 UDP + sucrose 6-phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharum officinarum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
DE52 anion exchange cellulose chromatography and His tag affinity column chromatography Saccharum officinarum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-alpha-D-glucose + D-fructose 6-phosphate
-
 Saccharum officinarum UDP + sucrose 6-phosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 120000, SDS-PAGE Saccharum officinarum

Synonyms

Synonyms Comment Organism
SPS1
-
 Saccharum officinarum
sucrose phosphate synthase
-
 Saccharum officinarum