Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of multiple knockout mutants of the four Arabidopsis sucrose phosphate synthase isozymes SPSA1, SPSA2, SPSB, and SPSC (T-DNA insertion mutants spsa1 (SALK 119162), spsa2 (SALK 064922), spsb (GABI 368F01) and spsc (SAIL 31 H05) and mutants spsa1/spsa2, spsa1/spsb, spsa1/spsc, spsa2/spsb, spsa2/spsc, spsb/spsc, spsa1/spsb/spsc, spsa1/spsa2/spsb, spsa1/spsa2/spsc, spsa2/spsb/spsc, and spsa1/spsa2/spsb/spsc). Despite their reduced SPS activity, mutants spsa1/spsa2, spsa1/spsb, spsa2/spsb, spsa2/spsc, spsb/spsc, spsa1/spsa2/spsb and spsa2/spsb/spsc display wild-type vegetative and reproductive morphology, and show wild-type photosynthetic capacity and respiration, phenotypes overview. Growth of rosettes, flowers and siliques of the spsa1/spsc and spsa1/spsa2/spsc mutants is reduced compared with wild-type plants. The plants display a high dark respiration phenotype. Mutant spsa1/spsb/spsc and spsa1/spsa2/spsb/spsc seeds poorly germinate and produce aberrant and sterile plants. Leaves of all viable sps mutants, except spsa1/spsc and spsa1/spsa2/spsc, accumulate wild-type levels of nonstructural carbohydrates. Mutant spsa1/spsc leaves possess high levels of metabolic intermediates and activities of enzymes of the glycolytic and tricarboxylic acid cycle pathways, and accumulate high levels of metabolic intermediates of the nocturnal starch-to-sucrose conversion process, even under continuous light conditions | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Arabidopsis thaliana | 5829 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q94BT0 | SPP3 or SPSB | - |
Arabidopsis thaliana | Q94BT0 | SPS1 or SPSA1 | - |
Arabidopsis thaliana | Q94BT0 | SPS2 or SPSA2 | - |
Arabidopsis thaliana | Q94BT0 | SPS4 or SPSC | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
flower | - |
Arabidopsis thaliana | - |
leaf | - |
Arabidopsis thaliana | - |
silique | - |
Arabidopsis thaliana | - |
Synonyms | Comment | Organism |
---|---|---|
SPS | - |
Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Arabidopsis thaliana |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme is essential for plant viability, the four SPS isozymes function in processes that are important for plant growth and nonstructural carbohydrate metabolism | Arabidopsis thaliana |