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Literature summary for 2.4.1.14 extracted from

  • Bahaji, A.; Baroja-Fernandez, E.; Ricarte-Bermejo, A.; Sanchez-Lopez, A.M.; Munoz, F.J.; Romero, J.M.; Ruiz, M.T.; Baslam, M.; Almagro, G.; Sesma, M.T.; Pozueta-Romero, J.
    Characterization of multiple SPS knockout mutants reveals redundant functions of the four Arabidopsis sucrose phosphate synthase isoforms in plant viability, and strongly indicates that enhanced respiration and accelerated starch turnover can alleviate the blockage of sucrose biosynthesis (2015), Plant Sci., 238, 135-147.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
additional information construction of multiple knockout mutants of the four Arabidopsis sucrose phosphate synthase isozymes SPSA1, SPSA2, SPSB, and SPSC (T-DNA insertion mutants spsa1 (SALK 119162), spsa2 (SALK 064922), spsb (GABI 368F01) and spsc (SAIL 31 H05) and mutants spsa1/spsa2, spsa1/spsb, spsa1/spsc, spsa2/spsb, spsa2/spsc, spsb/spsc, spsa1/spsb/spsc, spsa1/spsa2/spsb, spsa1/spsa2/spsc, spsa2/spsb/spsc, and spsa1/spsa2/spsb/spsc). Despite their reduced SPS activity, mutants spsa1/spsa2, spsa1/spsb, spsa2/spsb, spsa2/spsc, spsb/spsc, spsa1/spsa2/spsb and spsa2/spsb/spsc display wild-type vegetative and reproductive morphology, and show wild-type photosynthetic capacity and respiration, phenotypes overview. Growth of rosettes, flowers and siliques of the spsa1/spsc and spsa1/spsa2/spsc mutants is reduced compared with wild-type plants. The plants display a high dark respiration phenotype. Mutant spsa1/spsb/spsc and spsa1/spsa2/spsb/spsc seeds poorly germinate and produce aberrant and sterile plants. Leaves of all viable sps mutants, except spsa1/spsc and spsa1/spsa2/spsc, accumulate wild-type levels of nonstructural carbohydrates. Mutant spsa1/spsc leaves possess high levels of metabolic intermediates and activities of enzymes of the glycolytic and tricarboxylic acid cycle pathways, and accumulate high levels of metabolic intermediates of the nocturnal starch-to-sucrose conversion process, even under continuous light conditions Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Arabidopsis thaliana 5829
-

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q94BT0 SPP3 or SPSB
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Arabidopsis thaliana Q94BT0 SPS1 or SPSA1
-
Arabidopsis thaliana Q94BT0 SPS2 or SPSA2
-
Arabidopsis thaliana Q94BT0 SPS4 or SPSC
-

Source Tissue

Source Tissue Comment Organism Textmining
flower
-
Arabidopsis thaliana
-
leaf
-
Arabidopsis thaliana
-
silique
-
Arabidopsis thaliana
-

Synonyms

Synonyms Comment Organism
SPS
-
Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Arabidopsis thaliana

General Information

General Information Comment Organism
physiological function the enzyme is essential for plant viability, the four SPS isozymes function in processes that are important for plant growth and nonstructural carbohydrate metabolism Arabidopsis thaliana