Literature summary for 2.4.1.14 extracted from

Maloney, V.J.; Park, J.Y.; Unda, F.; Mansfield, S.D.
Sucrose phosphate synthase and sucrose phosphate phosphatase interact in planta and promote plant growth and biomass accumulation (2015), J. Exp. Bot., 66, 4383-4394.

Cloned(Commentary)

Cloned (Comment)	Organism
gene AtSPS5b, cloned with forward primer SPSa3 and reverse primer SPSb3, transfection via Agrobacterium tumefaciens strain EHA105 into Arabidopsis thaliana seedligs, transient recombinant expression of YFP-tagged enzyme in Nicotiana tabacum leaves and coexpression of sucrose phosphate phosphatase, recombinant expression in Saccharomyces cerevisiae strain MaV203 in a two-hybrid system with sucrose phosphate phosphatase, SPP, EC 3.1.3.24, AtSPP-AtSPS fusion is trabsfectde via Agrobacterium tumefaciens strain EHA105	Arabidopsis thaliana

Cloned (Comment)

Organism

gene AtSPS5b, cloned with forward primer SPSa3 and reverse primer SPSb3, transfection via Agrobacterium tumefaciens strain EHA105 into Arabidopsis thaliana seedligs, transient recombinant expression of YFP-tagged enzyme in Nicotiana tabacum leaves and coexpression of sucrose phosphate phosphatase, recombinant expression in Saccharomyces cerevisiae strain MaV203 in a two-hybrid system with sucrose phosphate phosphatase, SPP, EC 3.1.3.24, AtSPP-AtSPS fusion is trabsfectde via Agrobacterium tumefaciens strain EHA105

Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
UDP-glucose + D-fructose 6-phosphate	Arabidopsis thaliana	-	UDP + sucrose 6F-phosphate	-	?
UDP-glucose + D-fructose 6-phosphate	Arabidopsis thaliana Col-0	-	UDP + sucrose 6F-phosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Arabidopsis thaliana	Q94BT0	isozyme SPSA1; gene AtSPS5b or sps1	-
Arabidopsis thaliana Col-0	Q94BT0	isozyme SPSA1; gene AtSPS5b or sps1	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
UDP-glucose + D-fructose 6-phosphate	-	Arabidopsis thaliana	UDP + sucrose 6F-phosphate	-	?
UDP-glucose + D-fructose 6-phosphate	-	Arabidopsis thaliana Col-0	UDP + sucrose 6F-phosphate	-	?

Synonyms

Synonyms	Comment	Organism
SPS	-	Arabidopsis thaliana

General Information

General Information	Comment	Organism
additional information	sucrose phosphate synthase contains a putative C-terminal sucrose phosphate phosphatase-like domain that may facilitates the binding of sucrose phosphate phosphatase, EC 3.1.3.24, interaction analysis in transgenic plants and yeast two-hybrid system, overview	Arabidopsis thaliana