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Literature summary for 2.4.1.136 extracted from

  • Gross, G.
    Partial purification and properties of UDP-glucose: vanillate 1-O-glucosyl transferase from oak leaves (1983), Phytochemistry, 22, 2179-2182.
No PubMed abstract available
Search for more literature for 2.4.1.136

General Stability

General Stability Organism
2-mercaptoethanol in extraction buffers stabilizes Quercus rubra
diluted enzyme is unstable Quercus rubra

Inhibitors

Inhibitors Comment Organism Structure
dithiothreitol 4 mM, slight Quercus rubra
UDP strong Quercus rubra

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.57
-
 vanillate
-
 Quercus rubra
0.72
-
 veratrate
-
 Quercus rubra
1.11
-
 gallate
-
 Quercus rubra
3.45
-
 p-hydroxybenzoate
-
 Quercus rubra

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
68000
-
 gel filtration Quercus rubra

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDPglucose + gallate Quercus rubra physiological role probably is the formation of beta-glucogallin, the putative first intermediate in the biosynthesis of gallotannins UDP + 1-O-galloyl-beta-D-glucose i.e. beta-glucogallin r

Organism

Organism UniProt Comment Textmining
Quercus rubra
-
-
-

Oxidation Stability

Oxidation Stability Organism
O2-sensitive, mercaptoethanol stabilizes Quercus rubra

Purification (Commentary)

Purification (Comment) Organism
-
 Quercus rubra

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Quercus rubra
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0117
-
 reaction with gallate Quercus rubra

Storage Stability

Storage Stability Organism
0-4°C, 1-2 weeks Quercus rubra

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-alpha-D-glucose + 4-hydroxybenzoate 48% of activity compared to vanillate Quercus rubra UDP + 1-O-(4-hydroxybenzoyl)-beta-D-glucopyranose
-
 ?
UDP-alpha-D-glucose + cinnamate 9% of activity compared to vanillate Quercus rubra UDP + 1-O-cinnamoyl-beta-D-glucose
-
 ?
UDP-glucose + p-coumarate 32% of activity compared to vanillate Quercus rubra UDP + 1-O-p-coumaroyl-beta-D-glucose
-
 ?
UDPglucose + 3,4,5-trimethoxybenzoate 7% of activity compared to vanillate Quercus rubra UDP + 1-O-3,4,5-trimethoxybenzoyl-beta-D-glucose
-
 ?
UDPglucose + anisate 38% of activity compared to vanillate Quercus rubra UDP + 1-O-anisoyl-beta-D-glucose
-
 ?
UDPglucose + benzoate 16% of activity compared to vanillate Quercus rubra UDP + benzoyl-beta-D-glucose
-
 ?
UDPglucose + ferulate 35% of activity compared to vanillate Quercus rubra UDP + 1-O-feruloyl-beta-D-glucose
-
 ?
UDPglucose + gallate 52% of activity compared to vanillate Quercus rubra UDP + 1-O-galloyl-beta-D-glucose i.e. beta-glucogallin r
UDPglucose + gallate physiological role probably is the formation of beta-glucogallin, the putative first intermediate in the biosynthesis of gallotannins Quercus rubra UDP + 1-O-galloyl-beta-D-glucose i.e. beta-glucogallin r
UDPglucose + m-coumarate 14% of activity compared to vanillate Quercus rubra UDP + 1-O-m-coumaroyl-beta-D-glucose
-
 ?
UDPglucose + protocatechuate 22% of activity compared to vanillate Quercus rubra UDP + protocatechuyl-beta-D-glucose
-
 ?
UDPglucose + sinapate 13% of activity compared to vanillate Quercus rubra UDP + 1-O-sinapoyl-beta-D-glucose
-
 ?
UDPglucose + vanillate best substrate Quercus rubra UDP + vanilloyl-beta-D-glucose
-
 ?
UDPglucose + veratrate 71% of activity compared to vanillate Quercus rubra UDP + 1-O-veratroyl-beta-D-glucose
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
42
-
-
 Quercus rubra

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 7
-
 Quercus rubra

pH Range

pH Minimum pH Maximum Comment Organism
4.7 9 50% of maximal activity at pH 4.7 and 9.0 Quercus rubra