Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
A186D | the mutant exhibits 9times reduced activity compared to the wild type enzyme | Homo sapiens |
L206P | loss of activity upon L206P substitution due to altered protein folding is the primary cause for the glycosaminoglycan synthesis defect in patients with the progeroid form of Ehlers-Danlos syndrome carrying the compound | Homo sapiens |
R270C | the substitution strongly reduces enzyme affinity towards xyloside acceptor to about 60% compared to the wild type enzyme, thus affecting glycosaminoglycan chains formation | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.45 | - |
UDP-galactose | wild type enzyme, in 100 mM sodium cacodylate buffer (pH 7.4) containing 10 mM MnCl2, at 37°C | Homo sapiens | |
0.6 | - |
4-methylumbelliferyl O-beta-D-xylopyranoside | wild type enzyme, in 100 mM sodium cacodylate buffer (pH 7.4) containing 10 mM MnCl2, at 37°C | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
glutathione Sepharose column chromatography | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-galactose + 4-methylumbelliferyl O-beta-D-xylopyranoside | - |
Homo sapiens | UDP + 4-methylumbelliferyl 4-O-beta-D-galactopyranoyl-beta-D-xylopyranoside | - |
? |
Synonyms | Comment | Organism |
---|---|---|
beta1,4-galactosyltransferase 7 | - |
Homo sapiens |
beta4GalT7 | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.14 | - |
UDP-galactose | wild type enzyme, in 100 mM sodium cacodylate buffer (pH 7.4) containing 10 mM MnCl2, at 37°C | Homo sapiens |