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Literature summary for 2.4.1.133 extracted from

  • Ramakrishnan, B.; Qasba, P.K.
    Crystal structure of the catalytic domain of Drosophila beta1,4-Galactosyltransferase-7 (2010), J. Biol. Chem., 285, 15619-15626.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.1.133

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rosetta(DE3)pLysS cells Drosophila melanogaster

Crystallization (Commentary)

Crystallization (Comment) Organism
enzyme in the presence of manganese and UDP, hanging drop vapor diffusion method, using 100 mM Tris-HCl (pH 8.0), 1 M NaCl, 15% (v/v) MPD, and 5% (w/v) polyethylene glycol 6000 Drosophila melanogaster

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ the Drosophila enzyme exhibits only very low catalytic activity with magnesium Drosophila melanogaster
Mn2+ required Drosophila melanogaster

Organism

Organism UniProt Comment Textmining
Drosophila melanogaster Q8T3P3
-
-

Renatured (Commentary)

Renatured (Comment) Organism
100 mg of sulfonated protein are folded for 48 h in folding solution containing oxido-shuffling agents and 500 mM arginine-HCl Drosophila melanogaster

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-galactose + O-beta-D-xylosylprotein
-
 Drosophila melanogaster UDP + 4-beta-D-galactosyl-O-beta-D-xylosylprotein
-
 ?

Synonyms

Synonyms Comment Organism
beta1,4-galactosyltransferase-7
-
 Drosophila melanogaster
beta4Gal-T7
-
 Drosophila melanogaster