Cloned (Comment) | Organism |
---|---|
cloning and expression of human membrane beta1,4-GalT7 in HeLa cells, expression of soluble form as a fusion protein with maltose-binding protein in Escherichia coli | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CDP | - |
Homo sapiens | |
TDP | - |
Homo sapiens | |
UDP | - |
Homo sapiens | |
UDP-mannose | - |
Homo sapiens | |
UDP-N-acetylgalactosamine | - |
Homo sapiens | |
UTP | - |
Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
UDP-N-acetyl glucosamine | KM value is above 10 in 100 mM cacodylate buffer, pH 7.0 containing 10 mM MnCl2 and 0.2-6 microM purified fusion-beta1,4-GalT7 | Homo sapiens | |
additional information | - |
UDP-glucuronic acid | KM value is above 4 in 100 mM cacodylate buffer, pH 7.0 containing 10 mM MnCl2 and 0.2-6 microM purified fusion-beta1,4-GalT7 | Homo sapiens | |
additional information | - |
UDP-N-acetyl galactosamine | KM value is not detected in 100 mM cacodylate buffer, pH 7.0 containing 10 mM MnCl2 and 0.2-6 microM purified fusion-beta1,4-GalT7 | Homo sapiens | |
additional information | - |
UDP-mannose | KM value is not detected, in 100 mM cacodylate buffer, pH 7.0 containing 10 mM MnCl2 and 0.2-6 microM purified fusion-beta1,4-GalT7 | Homo sapiens | |
0.13 | - |
UDP-xylose | in 100 mM cacodylate buffer, pH 7.0 containing 10 mM MnCl2 and 0.2-6 microM purified fusion-beta1,4-GalT7 | Homo sapiens | |
0.16 | - |
4-methylumbelliferyl-beta-D-xylopyranoside | membrane beta1,4-GalT7 in HeLa cells | Homo sapiens | |
0.23 | - |
UDP-galactose | in 100 mM cacodylate buffer, pH 7.0 containing 10 mM MnCl2 and 0.2-6 microM purified fusion-beta1,4-GalT7 | Homo sapiens | |
0.27 | - |
4-methylumbelliferyl-beta-D-xylopyranoside | maltose-binding protein-beta1,4-GalT7 fusion protein | Homo sapiens | |
0.28 | - |
UDP-glucose | in 100 mM cacodylate buffer, pH 7.0 containing 10 mM MnCl2 and 0.2-6 microM purified fusion-beta1,4-GalT7 | Homo sapiens | |
0.31 | - |
UDP-galactose | membrane beta1,4-GalT7 in HeLa cells | Homo sapiens | |
0.85 | - |
4-nitrophenyl-beta-D-xylopyranoside | membrane beta1,4-GalT7 in HeLa cells | Homo sapiens | |
1.27 | - |
4-nitrophenyl-beta-D-xylopyranoside | maltose-binding protein-beta1,4-GalT7 fusion protein | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
Golgi membrane | - |
Homo sapiens | 139 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | enzyme catalyses the Mn2+-dependent transfer of galactose from UDP-galactose the non-reducing end of the xylose residue of the common tetrasaccharide linker region of glycosaminoglycans | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
70000 | - |
recombinant fusion protein, by SDS-PAGE | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-galactose + O-beta-D-xylosylprotein | Homo sapiens | - |
UDP + 4-beta-D-galactosyl-O-beta-D-xylosylprotein | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q9UBV7 | - |
- |
Purification (Comment) | Organism |
---|---|
purification of maltose-binding protein-beta1,4-GalT7 fusion protein with an amylose column | Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.058 | - |
maltose-binding protein-fusion enzyme, supernatant, in 100 mM cacodylate buffer containing 10 mM MnCl2, pH 7.0 | Homo sapiens |
0.97 | - |
maltose-binding protein-fusion enzyme, amylose elution, in 100 mM cacodylate buffer containing 10 mM MnCl2, pH 7.0 | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | enzyme is able to transfer glucose, xylose and, to a lesser extent glucuronic acid and N-acetylglucosamine, to acceptor sugars, whereas UDP-mannose and UDP-N-acetyl galactosamine are not substrates | Homo sapiens | ? | - |
? | |
UDP-galactose + O-beta-D-xylosylprotein | - |
Homo sapiens | UDP + 4-beta-D-galactosyl-O-beta-D-xylosylprotein | - |
? | |
UDP-glucose + 4-methylumbelliferyl-beta-D-xylopyranoside | - |
Homo sapiens | ? | - |
? | |
UDP-glucuronic acid + 4-methylumbelliferyl-beta-D-xylopyranoside | - |
Homo sapiens | ? | - |
? | |
UDP-N-acetyl glucosamine + 4-methylumbelliferyl-beta-D-xylopyranoside | - |
Homo sapiens | ? | - |
? | |
UDP-xylose + 4-methylumbelliferyl-beta-D-xylopyranoside | - |
Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
beta1,4-galactosyltransferase 7 | - |
Homo sapiens |
beta1,4-GalT | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
UDP-glucuronic acid | kcat value is above 0.2 1/min in 100 mM cacodylate buffer, pH 7.0 containing 10 mM MnCl2 and 0.2-6 microM purified fusion-beta1,4-GalT7 | Homo sapiens | |
additional information | - |
UDP-N-acetyl glucosamine | kcat value is above 0.2 1/min in 100 mM cacodylate buffer, pH 7.0 containing 10 mM MnCl2 and 0.2-6 microM purified fusion-beta1,4-GalT7 | Homo sapiens | |
additional information | - |
UDP-mannose | kcat value is not detected, in 100 mM cacodylate buffer, pH 7.0 containing 10 mM MnCl2 and 0.2-6 microM purified fusion-beta1,4-GalT7 | Homo sapiens | |
additional information | - |
UDP-N-acetyl galactosamine | kcat value not detected in 100 mM cacodylate buffer, pH 7.0 containing 10 mM MnCl2 and 0.2-6 microM purified fusion-beta1,4-GalT7 | Homo sapiens | |
3 | 6 | UDP-xylose | in 100 mM cacodylate buffer, pH 7.0 containing 10 mM MnCl2 and 0.2-6 microM purified fusion-beta1,4-GalT7 | Homo sapiens | |
72 | - |
UDP-glucose | in 100 mM cacodylate buffer, pH 7.0 containing 10 mM MnCl2 and 0.2-6 microM purified fusion-beta1,4-GalT7 | Homo sapiens | |
4080 | - |
4-methylumbelliferyl-beta-D-xylopyranoside | recombinant maltose-binding protein-beta1,4-GalT7 fusion protein | Homo sapiens | |
5086 | - |
UDP-galactose | in 100 mM cacodylate buffer, pH 7.0 containing 10 mM MnCl2 and 0.2-6 microM purified fusion-beta1,4-GalT7 | Homo sapiens | |
5460 | - |
4-nitrophenyl-beta-D-xylopyranoside | recombinant maltose-binding protein-beta1,4-GalT7 fusion protein | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
CDP | Ki value is above 9 mM, UDP-galactose 0.1-16 mM, 0-10 mM CDP | Homo sapiens | |
additional information | - |
UDP-N-acetyl galactosamine | Ki value is above 9 mM, UDP-galactose 0.1-16 mM, 0-10 mM UDP-N-acetyl galactosamine | Homo sapiens | |
additional information | - |
UDP-mannose | Ki value is above 9, UDP-galactose 0.1-16 mM, 0-10 mM UDP-mannose | Homo sapiens | |
0.61 | - |
UDP | UDP-galactose 0.1-16 mM, UDP 0-2 mM | Homo sapiens | |
1.65 | - |
UTP | UDP-galactose 0.1-16 mM, UTP 0-4 mM | Homo sapiens | |
1.93 | - |
TDP | UDP-galactose 0.1-16 mM, TDP 0-4 mM | Homo sapiens |
General Information | Comment | Organism |
---|---|---|
physiological function | enzyme is involved in the biosynthesis of the tetrasaccharide linker protein region (glucuronic acid beta1,3galactose beta1,3galactose beta1,4xylose beta1) of proteoglycans, by catalysing the transfer of galactose from the uridine 5-diphosphogalactose to a xylose residue, this reaction is rate-limiting in glycosaminoglycan biosynthesis | Homo sapiens |