General Stability | Organism |
---|---|
dialysis against detergent-free buffer, +/- 0.25 M KCl inactivates, dialysis against 1% Nonidet P-40 and 0.25 M KCl restores activity, even after a period of 18 h in the absence of detergent | Gallus gallus |
Nonidet P-40 stabilizes solubilized enzyme | Gallus gallus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Gallus gallus | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | requirement, 15 mM | Gallus gallus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDPgalactose + O-beta-D-xylosylprotein | Gallus gallus | involved in chondroitin sulfate biosynthesis | UDP + 4-beta-D-galactosyl-O-beta-D-xylosylprotein | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gallus gallus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
solubilized and partially purified, affinity chromatography | Gallus gallus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cartilage | epiphyses from embryonic femurs and tibiae | Gallus gallus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-alpha-D-galactose + D-xylose | - |
Gallus gallus | UDP + 4-O-beta-D-galactosyl-beta-D-xylose | - |
? | |
UDPgalactose + O-beta-D-xylosylprotein | involved in chondroitin sulfate biosynthesis | Gallus gallus | UDP + 4-beta-D-galactosyl-O-beta-D-xylosylprotein | - |
? | |
UDPgalactose + xylosylserine | - |
Gallus gallus | UDP + beta-D-galactosyl-1,4-beta-D-xylosylserine | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Gallus gallus |