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Literature summary for 2.4.1.13 extracted from
- Sequence determinants of nucleotide binding in sucrose synthase improving the affinity of a bacterial sucrose synthase for UDP by introducing plant residues (2017), Protein Eng. Des. Sel., 30, 141-148 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Acidithiobacillus caldus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| K639R | the mutant exhibits a strongly decreased Km value for UDP compared to the wild type enzyme | Acidithiobacillus caldus |
| L636Q/A642N | the mutant exhibits a strongly decreased Km value for UDP compared to the wild type enzyme | Acidithiobacillus caldus |
| L636Q/K639R/V641R/A642N | the mutant exhibits a strongly decreased Km value for UDP compared to the wild type enzyme | Acidithiobacillus caldus |
| L636Q/L637M/K639R/T640V/V641R/A642N | the mutant exhibits a strongly decreased Km value for UDP and increased Km for ADP as compared to the wild type enzyme | Acidithiobacillus caldus |
| L637M | the mutant exhibits a strongly decreased Km value for UDP compared to the wild type enzyme | Acidithiobacillus caldus |
| L637M/K639R/T640V | the mutant exhibits a strongly decreased Km value for UDP compared to the wild type enzyme | Acidithiobacillus caldus |
| L637M/T640V | the mutant exhibits a strongly decreased Km value for UDP compared to the wild type enzyme | Acidithiobacillus caldus |
| T640V | the mutant exhibits a strongly decreased Km value for UDP compared to the wild type enzyme | Acidithiobacillus caldus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| UDP | the wild type enzyme shows no significant inhibition of activity below 20 mM UDP | Acidithiobacillus caldus |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.3 | - |
ADP | at pH 7.0 and 60°C | Acidithiobacillus caldus | |
| 7.8 | - |
UDP | at pH 7.0 and 60°C | Acidithiobacillus caldus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| NDP + sucrose | Acidithiobacillus caldus | - |
NDP-alpha-D-glucose + D-fructose | - |
r | |
| NDP + sucrose | Acidithiobacillus caldus ATCC 51756 | - |
NDP-alpha-D-glucose + D-fructose | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Acidithiobacillus caldus | A0A059ZV61 | - |
- |
| Acidithiobacillus caldus ATCC 51756 | A0A059ZV61 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA column chromatography | Acidithiobacillus caldus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ADP + sucrose | - |
Acidithiobacillus caldus | ADP-alpha-D-glucose + D-fructose | - |
r | |
| ADP + sucrose | - |
Acidithiobacillus caldus ATCC 51756 | ADP-alpha-D-glucose + D-fructose | - |
r | |
| NDP + sucrose | - |
Acidithiobacillus caldus | NDP-alpha-D-glucose + D-fructose | - |
r | |
| NDP + sucrose | - |
Acidithiobacillus caldus ATCC 51756 | NDP-alpha-D-glucose + D-fructose | - |
r | |
| UDP + sucrose | - |
Acidithiobacillus caldus | UDP-alpha-D-glucose + D-fructose | - |
r | |
| UDP + sucrose | - |
Acidithiobacillus caldus ATCC 51756 | UDP-alpha-D-glucose + D-fructose | - |
r | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| SuSy | - |
Acidithiobacillus caldus |
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Release 2023.1 (January 2023)
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