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Literature summary for 2.4.1.13 extracted from

  • Figueroa, C.; Asencion Diez, M.; Kuhn, M.; McEwen, S.; Salerno, G.; Iglesias, A.; Ballicora, M.
    The unique nucleotide specificity of the sucrose synthase from Thermosynechococcus elongatus (2013), FEBS Lett., 587, 165-169.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
the gene is artificially synthesized for optimal recombinant expression in Escherichia coli strain Tuner (DE3) Thermosynechococcus vestitus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.033
-
ADP-glucose pH 7.0, 37°C, recombinant enzyme Thermosynechococcus vestitus
0.18
-
ADP pH 7.0, 37°C, recombinant enzyme Thermosynechococcus vestitus
0.9
-
CDP pH 7.0, 37°C, recombinant enzyme Thermosynechococcus vestitus
1.1
-
GDP pH 7.0, 37°C, recombinant enzyme Thermosynechococcus vestitus
1.3
-
UDP pH 7.0, 37°C, recombinant enzyme Thermosynechococcus vestitus
1.4
-
TDP pH 7.0, 37°C, recombinant enzyme Thermosynechococcus vestitus
1.7
-
UDP-glucose pH 7.0, 37°C, recombinant enzyme Thermosynechococcus vestitus
5.6
-
D-fructose pH 7.0, 37°C, recombinant enzyme, with ADP-glucose Thermosynechococcus vestitus
12
-
D-fructose pH 7.0, 37°C, recombinant enzyme, with UDP-glucose Thermosynechococcus vestitus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Thermosynechococcus vestitus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
95000
-
4 * 95000, recombinant enzyme, SDS-PAGE Thermosynechococcus vestitus
341000
-
recombinant enzyme, gel filtration Thermosynechococcus vestitus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
NDP-glucose + D-fructose Thermosynechococcus vestitus
-
NDP + sucrose
-
r

Organism

Organism UniProt Comment Textmining
Thermosynechococcus vestitus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP-glucose + D-fructose
-
Thermosynechococcus vestitus ADP + sucrose ADP is the best acceptor substrate in sucrose cleavage r
CDP-glucose + D-fructose
-
Thermosynechococcus vestitus CDP + sucrose
-
r
GDP-glucose + D-fructose
-
Thermosynechococcus vestitus GDP + sucrose
-
r
additional information unique nucleotide specificity of the sucrose synthase from Thermosynechococcus elongatus Thermosynechococcus vestitus ?
-
?
NDP-glucose + D-fructose
-
Thermosynechococcus vestitus NDP + sucrose
-
r
TDP-glucose + D-fructose lower activity in sucrose cleavage Thermosynechococcus vestitus TDP + sucrose
-
r
UDP-glucose + D-fructose lower activity in sucrose cleavage Thermosynechococcus vestitus UDP + sucrose
-
r

Subunits

Subunits Comment Organism
homotetramer 4 * 95000, recombinant enzyme, SDS-PAGE Thermosynechococcus vestitus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
sucrose cleavage with UDP Thermosynechococcus vestitus
70
-
sucrose cleavage with ADP Thermosynechococcus vestitus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 75 activity range, profile overview Thermosynechococcus vestitus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
the enzyme remains stable after 10 min incubation up to 50°C, but activity sharply decays beyond 55°C. Addition of 1 mM ADP, 10 mM UDP, or 200 mM Suc enhances the thermal stability by almost 5°C Thermosynechococcus vestitus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6
-
sucrose pH 7.0, 37°C, purified recombinant enzyme, with ADP in sucrose cleavage Thermosynechococcus vestitus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
sucrose cleavage with CDP, GDP, TDP, and UDP Thermosynechococcus vestitus
8
-
sucrose cleavage with ADP Thermosynechococcus vestitus

pH Range

pH Minimum pH Maximum Comment Organism
7 9 sucrose cleavage, activities with CDP, GDP, TDP, and UDP only occur at pH 7.0-8.0 and are very low at pH 9.0, while the activity with ADP is highest at pH 8.0 and higher than 60% of maximal activity in the pH range of 7.0-9.0 Thermosynechococcus vestitus

General Information

General Information Comment Organism
metabolism interaction between sucrose and glycogen metabolism Thermosynechococcus vestitus
physiological function in filamentous cyanobacteria, the sucrose cleavage direction plays a key physiological function in carbon metabolism, nitrogen fixation, and stress tolerance Thermosynechococcus vestitus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.38
-
D-fructose pH 7.0, 37°C, recombinant enzyme, with UDP-glucose Thermosynechococcus vestitus
0.406
-
D-fructose pH 7.0, 37°C, recombinant enzyme, with ADP-glucose Thermosynechococcus vestitus
2.673
-
UDP-glucose pH 7.0, 37°C, recombinant enzyme Thermosynechococcus vestitus
70.09
-
ADP-glucose pH 7.0, 37°C, recombinant enzyme Thermosynechococcus vestitus