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Literature summary for 2.4.1.13 extracted from

  • Römer, U.; Schrader, H.; Gunther, N.; Nettelstroth, N.; Frommer, W.B.; Elling, L.
    Expression, purification and characterization of recombinant sucrose synthase 1 from Solanum tuberosum L. for carbohydrate engineering (2004), J. Biotechnol., 107, 135-149.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
constitutive expression in Saccharomyces cerevisiae strain 22574dsus1 Solanum tuberosum

General Stability

General Stability Organism
no activity is lost during 3 freeze-thaw-cycles Solanum tuberosum

Inhibitors

Inhibitors Comment Organism Structure
2,5-dihydroxymethyl-(3S,4R)-dihydroxypyrrolidine
-
Solanum tuberosum
5-deoxy-beta-D-fructose competitive to D-fructose Solanum tuberosum
Co2+ 0.05 mM, 20% loss of activity Solanum tuberosum
Cu2+
-
Solanum tuberosum
D-fructose
-
Solanum tuberosum
Ni2+
-
Solanum tuberosum
sucrose
-
Solanum tuberosum
UDP
-
Solanum tuberosum
UDP-glucose
-
Solanum tuberosum
Zn2+
-
Solanum tuberosum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0041
-
UDP pH 7.6, 30°C Solanum tuberosum
0.012
-
dTDP pH 7.6, 30°C Solanum tuberosum
0.0916
-
sucrose pH 7.6, 30°C Solanum tuberosum
0.5
-
UDP-glucose pH 8.0, 30°C Solanum tuberosum
0.77
-
UDP-GlcNAc pH 8.0, 30°C Solanum tuberosum
2.1
-
D-fructose pH 8.0, 30°C Solanum tuberosum

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 20 mM, 2.5fold stimulation Solanum tuberosum
Mg2+ 20 mM, 3fold stimulation Solanum tuberosum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
90300
-
4 * 90300, SDS-PAGE Solanum tuberosum
394000
-
gel filtration Solanum tuberosum

Organism

Organism UniProt Comment Textmining
Solanum tuberosum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Solanum tuberosum

Storage Stability

Storage Stability Organism
-20°C, pH 7.5, stored for at least 6 months with 10% loss of activity Solanum tuberosum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
TDP-glucose + D-fructose
-
Solanum tuberosum TDP + sucrose
-
?
UDP + sucrose
-
Solanum tuberosum UDP-glucose + D-fructose
-
?
UDP-N-acetyl-alpha-D-glucosamine + D-fructose
-
Solanum tuberosum UDP + 2-acetamido-2-deoxy-D-glucopyranosyl-beta-D-fructofuranoside
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 90300, SDS-PAGE Solanum tuberosum

Synonyms

Synonyms Comment Organism
sucrose synthase 1
-
Solanum tuberosum
SuSy1
-
Solanum tuberosum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
56
-
-
Solanum tuberosum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
pH 7.6, 200 mM HEPES-NaOH buffer, 48 h, 20% loss of activity Solanum tuberosum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
cleavage of sucrose Solanum tuberosum
8
-
synthesis of sucrose Solanum tuberosum

pH Stability

pH Stability pH Stability Maximum Comment Organism
7.5
-
-20°C, 6 months, 10% loss of activity Solanum tuberosum

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.015
-
Cu2+
-
Solanum tuberosum
0.025
-
Zn2+
-
Solanum tuberosum
0.035
-
Ni2+
-
Solanum tuberosum
0.11
-
UDP
-
Solanum tuberosum
0.3
-
5-deoxy-beta-D-fructose
-
Solanum tuberosum
2.3
-
UDP-glucose
-
Solanum tuberosum
35.9
-
D-fructose
-
Solanum tuberosum

pI Value

Organism Comment pI Value Maximum pI Value
Solanum tuberosum
-
-
5.5