BRENDA - Enzyme Database
show all sequences of 2.4.1.13

Sucrose metabolism in cyanobacteria: sucrose synthase from Anabaena sp. strain PCC 7119 is remarkably different from the plant enzymes with respect to substrate affinity and amino-terminal sequence

Porchia, A.C.; Curatti, L.; Salerno, G.L.; Planta 210, 34-40 (1999)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
Mg2+
2-4fold activation of sucrose synthesis, not cleavage
Anabaena sp.
Mn2+
2-4fold activation of sucrose synthesis, inhibition of cleavage
Anabaena sp.
Inhibitors
Inhibitors
Commentary
Organism
Structure
arbutin
no enzyme activity at 5 mM
Anabaena sp.
fructose
above 20 mM, cosubstrate ADP-Glc, not with UDP-GLC
Anabaena sp.
Mn2+
sucrose cleavage
Anabaena sp.
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.15
-
ADP
-
Anabaena sp.
1.25
-
UDP
-
Anabaena sp.
3.7
-
CDP
-
Anabaena sp.
4.5
-
GDP
-
Anabaena sp.
288
303
sucrose
-
Anabaena sp.
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
360000
-
gel filtration, form SS-II
Anabaena sp.
Organism
Organism
UniProt
Commentary
Textmining
Anabaena sp.
-
strain PCC 7119, two isoforms
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Anabaena sp.
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
ADP-glucose + D-fructose
-
488567
Anabaena sp.
ADP + sucrose
-
-
-
r
CDP-glucose + D-fructose
-
488567
Anabaena sp.
CDP + sucrose
-
-
-
r
GDP-glucose + D-fructose
-
488567
Anabaena sp.
GDP + sucrose
-
-
-
r
UDP + sucrose
-
488567
Anabaena sp.
UDP-glucose + D-fructose
-
-
-
?
UDPglucose + D-fructose
-
488567
Anabaena sp.
UDP + sucrose
-
488567
Anabaena sp.
r
Subunits
Subunits
Commentary
Organism
tetramer
4 x 92000, SDS-PAGE, form SS-II, N-terminal sequence
Anabaena sp.
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1
1.5
Mn2+
sucrose cleavage
Anabaena sp.
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
Mg2+
2-4fold activation of sucrose synthesis, not cleavage
Anabaena sp.
Mn2+
2-4fold activation of sucrose synthesis, inhibition of cleavage
Anabaena sp.
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
arbutin
no enzyme activity at 5 mM
Anabaena sp.
fructose
above 20 mM, cosubstrate ADP-Glc, not with UDP-GLC
Anabaena sp.
Mn2+
sucrose cleavage
Anabaena sp.
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1
1.5
Mn2+
sucrose cleavage
Anabaena sp.
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.15
-
ADP
-
Anabaena sp.
1.25
-
UDP
-
Anabaena sp.
3.7
-
CDP
-
Anabaena sp.
4.5
-
GDP
-
Anabaena sp.
288
303
sucrose
-
Anabaena sp.
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
360000
-
gel filtration, form SS-II
Anabaena sp.
Purification (Commentary) (protein specific)
Commentary
Organism
-
Anabaena sp.
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
ADP-glucose + D-fructose
-
488567
Anabaena sp.
ADP + sucrose
-
-
-
r
CDP-glucose + D-fructose
-
488567
Anabaena sp.
CDP + sucrose
-
-
-
r
GDP-glucose + D-fructose
-
488567
Anabaena sp.
GDP + sucrose
-
-
-
r
UDP + sucrose
-
488567
Anabaena sp.
UDP-glucose + D-fructose
-
-
-
?
UDPglucose + D-fructose
-
488567
Anabaena sp.
UDP + sucrose
-
488567
Anabaena sp.
r
Subunits (protein specific)
Subunits
Commentary
Organism
tetramer
4 x 92000, SDS-PAGE, form SS-II, N-terminal sequence
Anabaena sp.
Other publictions for EC 2.4.1.13
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)