BRENDA - Enzyme Database
show all sequences of 2.4.1.13

The purification and properties of sucrose synthetase from etiolated Phaseolus aureus seedlings

Delmer, D.P.; J. Biol. Chem. 247, 3822-3828 (1972)

Data extracted from this reference:

General Stability
General Stability
Organism
DTT, 0.1 mM, stabilizes
Vigna radiata var. radiata
EDTA, 0.1 mM, stabilizes
Vigna radiata var. radiata
freeze-thawing inactivates
Vigna radiata var. radiata
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.17
0.2
GDP
-
Vigna radiata var. radiata
0.3
0.38
UDPglucose
cosubstrate TDP
Vigna radiata var. radiata
0.44
0.9
CDP
-
Vigna radiata var. radiata
16.9
40
sucrose
cosubstrate ADP
Vigna radiata var. radiata
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
additional information
-
amino acid composition
Vigna radiata var. radiata
94000
-
4 * 94000, SDS-PAGE
Vigna radiata var. radiata
375000
-
low speed sedimentation equilibrium centrifugation
Vigna radiata var. radiata
405000
-
gel filtration
Vigna radiata var. radiata
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
UDP + sucrose
Vigna radiata var. radiata
-
UDP-glucose + D-fructose
-
-
r
Organism
Organism
UniProt
Commentary
Textmining
Vigna radiata var. radiata
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Vigna radiata var. radiata
Reaction
Reaction
Commentary
Organism
Reaction ID
NDP-alpha-D-glucose + D-fructose = NDP + sucrose
mechanism
Vigna radiata var. radiata
Source Tissue
Source Tissue
Commentary
Organism
Textmining
seedling
-
Vigna radiata var. radiata
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Vigna radiata var. radiata
Storage Stability
Storage Stability
Organism
4°C, 0.1 mM EDTA and DTT, more than 50% of activity retained after 1 month
Vigna radiata var. radiata
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
ADP-glucose + D-fructose
-
488551
Vigna radiata var. radiata
ADP + sucrose
-
-
-
r
CDP-glucose + D-fructose
-
488551
Vigna radiata var. radiata
CDP + sucrose
-
-
-
r
GDP-glucose + D-fructose
-
488551
Vigna radiata var. radiata
GDP + sucrose
-
-
-
r
TDP-glucose + D-fructose
-
488551
Vigna radiata var. radiata
TDP + sucrose
-
-
-
r
UDP + sucrose
-
488551
Vigna radiata var. radiata
UDP-glucose + D-fructose
-
-
-
r
UDP + sucrose
also substrate: ADP
488551
Vigna radiata var. radiata
UDP-glucose + D-fructose
-
488551
Vigna radiata var. radiata
?
UDPglucose + D-fructose
-
488551
Vigna radiata var. radiata
UDP + sucrose
-
488551
Vigna radiata var. radiata
r
Subunits
Subunits
Commentary
Organism
tetramer
4 * 94000, SDS-PAGE
Vigna radiata var. radiata
General Stability (protein specific)
General Stability
Organism
DTT, 0.1 mM, stabilizes
Vigna radiata var. radiata
EDTA, 0.1 mM, stabilizes
Vigna radiata var. radiata
freeze-thawing inactivates
Vigna radiata var. radiata
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.17
0.2
GDP
-
Vigna radiata var. radiata
0.3
0.38
UDPglucose
cosubstrate TDP
Vigna radiata var. radiata
0.44
0.9
CDP
-
Vigna radiata var. radiata
16.9
40
sucrose
cosubstrate ADP
Vigna radiata var. radiata
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
additional information
-
amino acid composition
Vigna radiata var. radiata
94000
-
4 * 94000, SDS-PAGE
Vigna radiata var. radiata
375000
-
low speed sedimentation equilibrium centrifugation
Vigna radiata var. radiata
405000
-
gel filtration
Vigna radiata var. radiata
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
UDP + sucrose
Vigna radiata var. radiata
-
UDP-glucose + D-fructose
-
-
r
Purification (Commentary) (protein specific)
Commentary
Organism
-
Vigna radiata var. radiata
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
seedling
-
Vigna radiata var. radiata
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Vigna radiata var. radiata
Storage Stability (protein specific)
Storage Stability
Organism
4°C, 0.1 mM EDTA and DTT, more than 50% of activity retained after 1 month
Vigna radiata var. radiata
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
ADP-glucose + D-fructose
-
488551
Vigna radiata var. radiata
ADP + sucrose
-
-
-
r
CDP-glucose + D-fructose
-
488551
Vigna radiata var. radiata
CDP + sucrose
-
-
-
r
GDP-glucose + D-fructose
-
488551
Vigna radiata var. radiata
GDP + sucrose
-
-
-
r
TDP-glucose + D-fructose
-
488551
Vigna radiata var. radiata
TDP + sucrose
-
-
-
r
UDP + sucrose
-
488551
Vigna radiata var. radiata
UDP-glucose + D-fructose
-
-
-
r
UDP + sucrose
also substrate: ADP
488551
Vigna radiata var. radiata
UDP-glucose + D-fructose
-
488551
Vigna radiata var. radiata
?
UDPglucose + D-fructose
-
488551
Vigna radiata var. radiata
UDP + sucrose
-
488551
Vigna radiata var. radiata
r
Subunits (protein specific)
Subunits
Commentary
Organism
tetramer
4 * 94000, SDS-PAGE
Vigna radiata var. radiata
Other publictions for EC 2.4.1.13
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)