Activating Compound | Comment | Organism | Structure |
---|---|---|---|
benzylpenicillin | stimulation, in the presence of 15% methanol, not at higher methanol concentrations or in the presence of deoxycholate | Escherichia coli | |
Dimethylsulfoxide | activation, in the absence of methanol, inhibits in the presence of 0.05% sarkosyl | Escherichia coli | |
EDTA | stimulation in the presence of high concentrations of methanol and detergents | Escherichia coli | |
Imipenem | stimulation, in the presence of 15% methanol, not at higher methanol concentrations or in the presence of deoxycholate | Escherichia coli | |
additional information | no activation by cephalexin, nocardicin A or mecillinam | Escherichia coli | |
Sarkosyl | activation | Escherichia coli | |
Sodium 1,2-cyclohexanediamine-N,N,N',N'-tetraacetic acid | stimulation in the presence of high concentrations of methanol and detergents | Escherichia coli | |
sodium deoxycholate | 0.05-0.1%, activation | Escherichia coli | |
Triton X-100 | activation, 0.05% | Escherichia coli | |
Triton X-100 | inhibits at 0.1% | Escherichia coli |
Cloned (Comment) | Organism |
---|---|
Escherichia coli structural gene mrcB, recloned from plasmid pLC19-19 to high copy number plasmid pBr322, yielding plasmid pTM13 | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | - |
Escherichia coli | |
Co2+ | - |
Escherichia coli | |
Dimethylsulfoxide | in the presence of 0.05% N-lauroylsarcosine | Escherichia coli | |
EDTA | in the absence of detergents, stimulates in the presence of high concentrations of methanol and detergents | Escherichia coli | |
enramycin | - |
Escherichia coli | |
Fe2+ | - |
Escherichia coli | |
macarbomycin | - |
Escherichia coli | |
Mn2+ | - |
Escherichia coli | |
Moenomycin | - |
Escherichia coli | |
Ni2+ | - |
Escherichia coli | |
Sodium 1,2-cyclohexanediamine-N,N,N',N'-tetraacetic acid | in the absence of detergents, stimulates in the presence of high concentrations of methanol and detergents | Escherichia coli | |
sodium deoxycholate | in the presence of methanol, inhibits at 0.5% | Escherichia coli | |
Triton X-100 | inhibits at 0.1% | Escherichia coli | |
Vancomycin | - |
Escherichia coli | |
Zn2+ | - |
Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Escherichia coli | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | slight stimulation | Escherichia coli | |
additional information | no divalent cation requirement | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
90000 | - |
x * 90000, Escherichia coli penicillin binding protein 1B alpha, beta or gamma | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Escherichia coli JA200/pLC19-19 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
3 isozymes | Escherichia coli |
Storage Stability | Organism |
---|---|
-80°C, in concentrated PEG 6000 solution, stable for several months | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
[GlcNAc-(1-4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)]n-diphosphoundecaprenol + GlcNAc-(1-4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol | - |
Escherichia coli | [GlcNAc-(1-4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)]n+1-diphosphoundecaprenol + undecaprenyl diphosphate | - |
? | |
[GlcNAc-(1-4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)]n-diphosphoundecaprenol + GlcNAc-(1-4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol | - |
Escherichia coli JA200/pLC19-19 | [GlcNAc-(1-4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)]n+1-diphosphoundecaprenol + undecaprenyl diphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 90000, Escherichia coli penicillin binding protein 1B alpha, beta or gamma | Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Escherichia coli |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 45 | about 60% of maximal activity at 20°C and about half-maximal activity at 45°C | Escherichia coli |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
up to 87% loss of activity within 10 min | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 9 | broad, in the presence of 0.1% deoxycholate | Escherichia coli |
8.5 | - |
Tris-HCl buffer without deoxycholate | Escherichia coli |