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Literature summary for 2.4.1.122 extracted from

  • Perrine, C.; Ju, T.; Cummings, R.D.; Gerken, T.A.
    Systematic determination of the peptide acceptor preferences for the human UDP-Gal:glycoprotein-alpha-GalNAc beta 3 galactosyltransferase (T-synthase) (2009), Glycobiology, 19, 321-328.
    View publication on PubMedView publication on EuropePMC

Metals/Ions

Metals/Ions Comment Organism Structure
CaCl2 0.1 mM are included in assay medium Homo sapiens
MnCl2 20 mM are included in assay medium Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens elevated preferences for Gly at the +1 position with moderately high preferences for Phe and Tyr in the +3 position relative to the acceptor Thr-O-GalNAc ?
-
?
UDP-galactose + glycoprotein N-acetyl-D-galactosamine Homo sapiens
-
UDP + glycoprotein D-galactosyl-(1->3)-N-acetyl-D-galactosamine
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
T-synthase may utilize charge-charge interactions to modulate its activity, rejecting basic peptides over neutral and acidic peptide acceptors, T-synthase recognizes specific features of the peptide portion of the substrate, including overall charge and the presence of Gly, Phe, Tyr, and perhaps Pro at specific positions, up to four residues from the site of glycosylation, and indeed may use these features to modulate sites of Core 1 glycosylation Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information elevated preferences for Gly at the +1 position with moderately high preferences for Phe and Tyr in the +3 position relative to the acceptor Thr-O-GalNAc Homo sapiens ?
-
?
UDP-galactose + glycoprotein N-acetyl-D-galactosamine
-
Homo sapiens UDP + glycoprotein D-galactosyl-(1->3)-N-acetyl-D-galactosamine
-
?

Synonyms

Synonyms Comment Organism
T-synthase
-
Homo sapiens
UDP-Gal:glycoprotein-alpha-GalNAc beta3 galactosyltransferase
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
assay at Homo sapiens

General Information

General Information Comment Organism
physiological function mucin-type protein O-glycosylation Homo sapiens