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Literature summary for 2.4.1.122 extracted from

  • Yoshida, H.; Fuwa, T.J.; Arima, M.; Hamamoto, H.; Sasaki, N.; Ichimiya, T.; Osawa, K.; Ueda, R.; Nishihara, S.
    Identification of the Drosophila core 1 beta1,3-galactosyltransferase gene that synthesizes T antigen in the embryonic central nervous system and hemocytes (2008), Glycobiology, 18, 1094-1104.
    View publication on PubMed
Search for more literature for 2.4.1.122

Metals/Ions

Metals/Ions Comment Organism Structure
MnCl2 20 mM are included in assay medium Drosophila melanogaster

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-galactose + glycoprotein N-acetyl-D-galactosamine Drosophila melanogaster
-
 UDP + glycoprotein D-galactosyl-(1->3)-N-acetyl-D-galactosamine
-
 ?

Organism

Organism UniProt Comment Textmining
Drosophila melanogaster Q7K237
-
-

Source Tissue

Source Tissue Comment Organism Textmining
central nervous system during embryogenesis, in late embryos at stage 16 Drosophila melanogaster
-
embryo
-
 Drosophila melanogaster
-
ML-DmBG3-c2 cell
-
 Drosophila melanogaster
-
S2 cell
-
 Drosophila melanogaster
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.000168
-
 the extracts of EY13370 third instar larvae have a galactosyltransferase activity of 44% of that of wild-type using para-nitrophenyl-N-acetyl-alpha-galactosaminide as the acceptor Drosophila melanogaster

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-galactose + glycoprotein N-acetyl-D-galactosamine
-
 Drosophila melanogaster UDP + glycoprotein D-galactosyl-(1->3)-N-acetyl-D-galactosamine
-
 ?

Synonyms

Synonyms Comment Organism
C1beta3GalT
-
 Drosophila melanogaster
core 1 beta1,3-galactosyltransferase
-
 Drosophila melanogaster
dC1beta3GalT1
-
 Drosophila melanogaster
T-synthase
-
 Drosophila melanogaster

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Drosophila melanogaster

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8
-
 assay at Drosophila melanogaster

General Information

General Information Comment Organism
malfunction mutant embryos show the loss of T antigens on the CNS and on a subset of hemocytes, 2 mutant lines, EY13370 and KG02976, have a P-element insertion in the CG9520 gene region, although the P-elements are located in the first intron of the gene in both lines, KG02976 homozygotes develop normally whereas some EY13370 homozygotes die during development and escaper homozygous EY13370 adult flies have abnormal legs, the levels of the enzyme transcripts in mutant third instar larvae homozygous for KG02976 or EY13370 are reduced to 60% and 15% of that of wild-type third instar larvae, terminal galactose of T antigen is defective in mutant embryos Drosophila melanogaster
physiological function enzyme is responsible for the synthesis and function of T antigen (mucin-type O-glycan) in vivo Drosophila melanogaster