Cloned (Comment) | Organism |
---|---|
gene UGT84A9, semi-quantitative RT-PCR, enzyme expression analysis | Brassica napus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of UGT84A9-suppressing (UGT84A9i) lines, suppression of UGT84A9 under control of the seed-specific NAPINC promoter is maintained in cotyledons during the first two weeks of seedling development. Compared to the wild-type, the UGT84A9i lines display a dramatically altered metabolic phenotype during late seed and early seedling development, in both UGT84A9i lines, the level of sinapine is reduced to about 50% of the wild type level, overview. Determination of metabolite levels in four developmental stages of wild-type and mutants subjected to hierarchical cluster analysis reveal seven metabolite groups | Brassica napus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Brassica napus | Q9FYU7 | gene UGT84A9 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seed | - |
Brassica napus | - |
seedling | - |
Brassica napus | - |
Synonyms | Comment | Organism |
---|---|---|
UDP-glucose:sinapic acid glucosyltransferase | - |
Brassica napus |
UGT84A9 | - |
Brassica napus |
General Information | Comment | Organism |
---|---|---|
malfunction | suppression of the key biosynthetic enzyme UDP-glucose:sinapic acid glucosyltransferase (UGT84A9) inhibits the biosynthesis of sinapine (sinapoylcholine), the major phenolic component of seeds. Suppression of the major sink pathway of sinapic acid impacts the metabolome of developing seeds and seedlings in UGT84A9-suppressing (UGT84A9i) lines, massive alterations become evident in late stages of seed development affecting the accumulation levels of 58 secondary and 7 primary metabolites, e.g. decreased amounts of various hydroxycinnamic acid esters, and increased formation of sinapic and syringic acid glycosides, overview. Suppression of UGT84A9 under control of the seed-specific NAPINC promoter is maintained in cotyledons during the first two weeks of seedling development and associated with a reduced and delayed transformation of sinapine into sinapoylmalate | Brassica napus |
physiological function | the enzyme is involved in glycosylation and beta-oxidation as metabolic detoxification strategies to bypass intracellular accumulation of sinapic acid. Feedback regulation of hydroxycinnamic acid biosynthesis | Brassica napus |