Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | - |
Brassica napus | |
Hg2+ | - |
Brassica napus | |
p-hydroxymercuribenzoate | - |
Brassica napus | |
TDP | - |
Brassica napus | |
UDP | - |
Brassica napus | |
UDPmannose | weak inhibition | Brassica napus | |
UDPxylose | weak inhibition | Brassica napus | |
Zn2+ | - |
Brassica napus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.16 | - |
Sinapic acid | - |
Brassica napus | |
2.4 | - |
UDPglucose | - |
Brassica napus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Brassica napus | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | modest stimulation | Brassica napus | |
Mn2+ | modest stimulation | Brassica napus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42000 | - |
gel filtration | Brassica napus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Brassica napus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Brassica napus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
UDP-alpha-D-glucose + sinapate = UDP + 1-O-sinapoyl-beta-D-glucose | mechanism fits a random bi bi model | Brassica napus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seedling | - |
Brassica napus | - |
Storage Stability | Organism |
---|---|
-20°C, litte loss of activity after 1 month | Brassica napus |
4°C, less than 10% loss of activity after 2 d, more than 90% loss of activity after 1 month | Brassica napus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dTDP-alpha-D-glucose + sinapic acid | 96% of the activity with UDPglucose | Brassica napus | dTDP + 1-O-sinapoyl-beta-D-glucose | - |
r | |
UDP-alpha-D-glucose + 4-coumaric acid | 21% of the activity with sinapic acid | Brassica napus | UDP + 1-O-(4-coumaroyl)-beta-D-glucose | - |
? | |
UDP-alpha-D-glucose + 5-hydroxyferulic acid | 39% of the activity with sinapic acid | Brassica napus | UDP + 1 O-(5-hydroxyferuloyl)-beta-D-glucose | - |
? | |
UDP-alpha-D-glucose + caffeic acid | 14% of the activity with sinapic acid | Brassica napus | UDP + 1-O-caffeoyl-beta-D-glucose | - |
? | |
UDP-alpha-D-glucose + cinnamic acid | 24% of the activity with sinapic acid | Brassica napus | UDP + 1-O-cinnamoyl-beta-D-glucose | - |
? | |
UDP-alpha-D-glucose + sinapic acid | - |
Brassica napus | UDP + 1-O-sinapoyl-beta-D-glucose | - |
r | |
UDP-alpha-D-glucose + syringic acid | 10% of the activity with sinapic acid | Brassica napus | UDP + 1-O-syringoyl-beta-D-glucose | - |
? | |
UDPalpha-D-glucose + ferulic acid | 77% of the activity with sinapid acid | Brassica napus | UDP + 1-O-feruloyl-beta-D-glucose | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
32 | - |
- |
Brassica napus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
32 | 50 | 32°C: maximal activity, 50°C: no activity above | Brassica napus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | - |
2 d, less than 10% loss of activity | Brassica napus |
25 | - |
24 h more than 90% loss of activity | Brassica napus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
MES buffer | Brassica napus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.5 | 8.5 | no activity detected below pH 4.5 and above pH 8.5 | Brassica napus |