Cloned (Comment) | Organism |
---|---|
expressed in Pichia pastoris | Populus tremula x Populus tremuloides |
Protein Variants | Comment | Organism |
---|---|---|
additional information | deletion of the enzyme's N-terminal RING-finger domain almost completely abolishes fiber formation but not cellulose biosynthetic activity | Populus tremula x Populus tremuloides |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | ADP and GDP do not inhibit the enzyme | Populus tremula x Populus tremuloides | |
UDP | - |
Populus tremula x Populus tremuloides |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
UDP-alpha-D-glucose | at pH 7.0 and 35°C | Populus tremula x Populus tremuloides |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | activates | Populus tremula x Populus tremuloides |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-alpha-D-glucose + [(1->4)-beta-D-glucosyl]n | Populus tremula x Populus tremuloides | - |
UDP + [(1->4)-beta-D-glucosyl]n+1 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Populus tremula x Populus tremuloides | - |
- |
- |
Purification (Comment) | Organism |
---|---|
cobalt affinity column chromatography | Populus tremula x Populus tremuloides |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-alpha-D-glucose + [(1->4)-beta-D-glucosyl]n | - |
Populus tremula x Populus tremuloides | UDP + [(1->4)-beta-D-glucosyl]n+1 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 100000, SDS-PAGE | Populus tremula x Populus tremuloides |
? | x * 110500, calculated from amino acid sequence | Populus tremula x Populus tremuloides |
Synonyms | Comment | Organism |
---|---|---|
cellulose synthase-8 | - |
Populus tremula x Populus tremuloides |
CESA8 | - |
Populus tremula x Populus tremuloides |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
- |
Populus tremula x Populus tremuloides |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Populus tremula x Populus tremuloides |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.027 | - |
UDP | at pH 7.0 and 35°C | Populus tremula x Populus tremuloides |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme is not only sufficient for cellulose biosynthesis in vitro but also suffices to bundle individual glucan chains into cellulose microfibrils | Populus tremula x Populus tremuloides |
physiological function | the enzyme is implicated in secondary cell wall formation | Populus tremula x Populus tremuloides |