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Literature summary for 2.4.1.12 extracted from

  • Purushotham, P.; Cho, S.; Diaz-Moreno, S.; Kumar, M.; Nixon, B.; Bulone, V.; Zimmer, J.
    A single heterologously expressed plant cellulose synthase isoform is sufficient for cellulose microfibril formation in vitro (2016), Proc. Natl. Acad. Sci. USA, 113, 11360-11365 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Pichia pastoris Populus tremula x Populus tremuloides

Protein Variants

Protein Variants Comment Organism
additional information deletion of the enzyme's N-terminal RING-finger domain almost completely abolishes fiber formation but not cellulose biosynthetic activity Populus tremula x Populus tremuloides

Inhibitors

Inhibitors Comment Organism Structure
additional information ADP and GDP do not inhibit the enzyme Populus tremula x Populus tremuloides
UDP
-
Populus tremula x Populus tremuloides

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.03
-
UDP-alpha-D-glucose at pH 7.0 and 35°C Populus tremula x Populus tremuloides

Localization

Localization Comment Organism GeneOntology No. Textmining

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ activates Populus tremula x Populus tremuloides

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-alpha-D-glucose + [(1->4)-beta-D-glucosyl]n Populus tremula x Populus tremuloides
-
UDP + [(1->4)-beta-D-glucosyl]n+1
-
?

Organism

Organism UniProt Comment Textmining
Populus tremula x Populus tremuloides
-
-
-

Purification (Commentary)

Purification (Comment) Organism
cobalt affinity column chromatography Populus tremula x Populus tremuloides

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-alpha-D-glucose + [(1->4)-beta-D-glucosyl]n
-
Populus tremula x Populus tremuloides UDP + [(1->4)-beta-D-glucosyl]n+1
-
?

Subunits

Subunits Comment Organism
? x * 100000, SDS-PAGE Populus tremula x Populus tremuloides
? x * 110500, calculated from amino acid sequence Populus tremula x Populus tremuloides

Synonyms

Synonyms Comment Organism
cellulose synthase-8
-
Populus tremula x Populus tremuloides
CESA8
-
Populus tremula x Populus tremuloides

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
Populus tremula x Populus tremuloides

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Populus tremula x Populus tremuloides

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.027
-
UDP at pH 7.0 and 35°C Populus tremula x Populus tremuloides

General Information

General Information Comment Organism
metabolism the enzyme is not only sufficient for cellulose biosynthesis in vitro but also suffices to bundle individual glucan chains into cellulose microfibrils Populus tremula x Populus tremuloides
physiological function the enzyme is implicated in secondary cell wall formation Populus tremula x Populus tremuloides