BRENDA - Enzyme Database
show all sequences of 2.4.1.117

Characterization of maize endosperm UDP-glucose:dolicholphosphate glucosyltransferase

Miernyk, J.A.; Riedell, W.E.; Phytochemistry 30, 2865-2867 (1991)
No PubMed abstract available

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
detergent
required for maximum activity, optimum for Triton X-100: 0.015%
Zea mays
Inhibitors
Inhibitors
Commentary
Organism
Structure
tunicamycin
50% inhibition at 0.04 mg per ml
Zea mays
UDP
-
Zea mays
UDP-glucuronic acid
-
Zea mays
UMP
-
Zea mays
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
-
Zea mays
0.0005
-
UDP-glucose
-
Zea mays
0.0012
-
dolichyl phosphate
-
Zea mays
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
-
Zea mays
-
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
Km: 0.7 mM
Zea mays
Organism
Organism
UniProt
Commentary
Textmining
Zea mays
-
L. inbred A 636
-
Purification (Commentary)
Purification (Commentary)
Organism
partial
Zea mays
Source Tissue
Source Tissue
Commentary
Organism
Textmining
endosperm
-
Zea mays
-
Storage Stability
Storage Stability
Organism
-70°C, up to 4 weeks
Zea mays
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
UDP-glucose + dolichyl phosphate
-
488487
Zea mays
UDP + dolichyl beta-D-glucosyl phosphate
-
-
-
?
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
-
Zea mays
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.0076
-
UDP-glucuronic acid
-
Zea mays
0.15
-
UDP
-
Zea mays
0.65
-
UMP
-
Zea mays
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
detergent
required for maximum activity, optimum for Triton X-100: 0.015%
Zea mays
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
tunicamycin
50% inhibition at 0.04 mg per ml
Zea mays
UDP
-
Zea mays
UDP-glucuronic acid
-
Zea mays
UMP
-
Zea mays
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.0076
-
UDP-glucuronic acid
-
Zea mays
0.15
-
UDP
-
Zea mays
0.65
-
UMP
-
Zea mays
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
-
Zea mays
0.0005
-
UDP-glucose
-
Zea mays
0.0012
-
dolichyl phosphate
-
Zea mays
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
-
Zea mays
-
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
Km: 0.7 mM
Zea mays
Purification (Commentary) (protein specific)
Commentary
Organism
partial
Zea mays
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
endosperm
-
Zea mays
-
Storage Stability (protein specific)
Storage Stability
Organism
-70°C, up to 4 weeks
Zea mays
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
UDP-glucose + dolichyl phosphate
-
488487
Zea mays
UDP + dolichyl beta-D-glucosyl phosphate
-
-
-
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
-
Zea mays
Other publictions for EC 2.4.1.117
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
686298
Haecker
Wollknauel is required for emb ...
Drosophila sp. (in: Insecta)
Development
135
1745-1749
2008
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1
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488488
Arroyo-Flores
Biosynthesis of glycoproteins ...
Candida albicans
Fungal Genet. Biol.
30
127-133
2000
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4
1
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488493
Lopez-Romero
-
Glycosyl transferases and glyc ...
Candida albicans
Recent Res. Dev. Microbiol.
4
667-681
2000
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488492
Rodriguez-Bonilla
Biosynthesis of glycoproteins ...
Candida albicans
Antonie van Leeuwenhoek
73
373-380
1998
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1
1
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3
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488489
Gartung
The submicrosomal localization ...
Homo sapiens
J. Hepatol.
20
32-40
1994
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488491
Heesen
Isolation of the ALG5 locus en ...
Saccharomyces cerevisiae
Eur. J. Biochem.
224
71-79
1994
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1
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1
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488490
Bossuyt
-
Topology of nucleotide-sugar:d ...
Rattus norvegicus
Biochem. J.
296
627-632
1993
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488486
Drake
Partial purificaton, photoaffi ...
Vigna radiata var. radiata
Plant Physiol.
97
396-401
1991
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2
2
1
2
1
1
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3
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488487
Miernyk
-
Characterization of maize endo ...
Zea mays
Phytochemistry
30
2865-2867
1991
1
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4
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1
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488484
Matern
Isolation and characterization ...
Homo sapiens
Eur. J. Biochem.
190
99-105
1990
1
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488485
Matern
Control of dolichyl phosphoglu ...
Homo sapiens
Biochim. Biophys. Acta
1004
67-72
1989
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1
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1
4
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488483
Riedell
Glycoprotein synthesis in maiz ...
Zea mays
Plant Physiol.
87
420-426
1988
2
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3
1
1
5
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1
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5
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488480
Villemez
Properties of a soluble polypr ...
Acanthamoeba castellanii
J. Biol. Chem.
254
4814-4819
1979
1
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488481
Herscovics
Glucosyltransferase activity i ...
Bos taurus
J. Biol. Chem.
252
2271-2277
1977
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488482
Behrens
Dolichol monophosphate glucose ...
Rattus norvegicus
Proc. Natl. Acad. Sci. USA
66
153-159
1970
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