BRENDA - Enzyme Database
show all sequences of 2.4.1.117

Partial purificaton, photoaffinity labeling, and properties of mung bean UDP-glucose:dolicholphosphate glucosyltransferase

Drake, R.R.; Kaushal, G.P.; Pastuszak, I.; Elbein, A.D.; Plant Physiol. 97, 396-401 (1991)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
EDTA
-
Vigna radiata var. radiata
MnCl2
stimulates at 1 mM, inhibits at higher concentration
Vigna radiata var. radiata
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.002
-
dolichyl phosphate
-
Vigna radiata var. radiata
0.027
-
UDP-glucose
-
Vigna radiata var. radiata
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
-
Vigna radiata var. radiata
-
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Ca2+
CaCl2 cannot replace MgCl2 or MnCl2
Vigna radiata var. radiata
Mn2+
MnCl2 stimulates at 1 mM, inhibits at higher concentration
Vigna radiata var. radiata
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
39000
-
x * 39000, SDS-PAGE after photoaffinity labeling, catalytic subunit
Vigna radiata var. radiata
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
additional information
Vigna radiata var. radiata
enzyme is involved in the biosynthesis of the lipid-linked oligosaccharides that are precursors of N-linked glycoproteins
?
-
-
-
Organism
Organism
UniProt
Commentary
Textmining
Vigna radiata var. radiata
-
mung bean
-
Purification (Commentary)
Purification (Commentary)
Organism
partial
Vigna radiata var. radiata
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Vigna radiata var. radiata
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
additional information
enzyme is involved in the biosynthesis of the lipid-linked oligosaccharides that are precursors of N-linked glycoproteins
488486
Vigna radiata var. radiata
?
-
-
-
-
UDP-glucose + dolichyl phosphate
-
488486
Vigna radiata var. radiata
UDP + dolichyl beta-D-glucosyl phosphate
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 39000, SDS-PAGE after photoaffinity labeling, catalytic subunit
Vigna radiata var. radiata
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Vigna radiata var. radiata
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
7
-
Vigna radiata var. radiata
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
EDTA
-
Vigna radiata var. radiata
MnCl2
stimulates at 1 mM, inhibits at higher concentration
Vigna radiata var. radiata
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.002
-
dolichyl phosphate
-
Vigna radiata var. radiata
0.027
-
UDP-glucose
-
Vigna radiata var. radiata
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
-
Vigna radiata var. radiata
-
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Ca2+
CaCl2 cannot replace MgCl2 or MnCl2
Vigna radiata var. radiata
Mn2+
MnCl2 stimulates at 1 mM, inhibits at higher concentration
Vigna radiata var. radiata
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
39000
-
x * 39000, SDS-PAGE after photoaffinity labeling, catalytic subunit
Vigna radiata var. radiata
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
additional information
Vigna radiata var. radiata
enzyme is involved in the biosynthesis of the lipid-linked oligosaccharides that are precursors of N-linked glycoproteins
?
-
-
-
Purification (Commentary) (protein specific)
Commentary
Organism
partial
Vigna radiata var. radiata
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Vigna radiata var. radiata
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
additional information
enzyme is involved in the biosynthesis of the lipid-linked oligosaccharides that are precursors of N-linked glycoproteins
488486
Vigna radiata var. radiata
?
-
-
-
-
UDP-glucose + dolichyl phosphate
-
488486
Vigna radiata var. radiata
UDP + dolichyl beta-D-glucosyl phosphate
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 39000, SDS-PAGE after photoaffinity labeling, catalytic subunit
Vigna radiata var. radiata
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Vigna radiata var. radiata
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
7
-
Vigna radiata var. radiata
Other publictions for EC 2.4.1.117
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
686298
Haecker
Wollknauel is required for emb ...
Drosophila sp. (in: Insecta)
Development
135
1745-1749
2008
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1
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488488
Arroyo-Flores
Biosynthesis of glycoproteins ...
Candida albicans
Fungal Genet. Biol.
30
127-133
2000
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4
1
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3
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1
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488493
Lopez-Romero
-
Glycosyl transferases and glyc ...
Candida albicans
Recent Res. Dev. Microbiol.
4
667-681
2000
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488492
Rodriguez-Bonilla
Biosynthesis of glycoproteins ...
Candida albicans
Antonie van Leeuwenhoek
73
373-380
1998
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1
1
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3
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1
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1
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1
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1
1
1
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3
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1
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488489
Gartung
The submicrosomal localization ...
Homo sapiens
J. Hepatol.
20
32-40
1994
-
-
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-
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1
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1
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1
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488491
Heesen
Isolation of the ALG5 locus en ...
Saccharomyces cerevisiae
Eur. J. Biochem.
224
71-79
1994
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1
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1
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1
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3
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1
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488490
Bossuyt
-
Topology of nucleotide-sugar:d ...
Rattus norvegicus
Biochem. J.
296
627-632
1993
-
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1
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488486
Drake
Partial purificaton, photoaffi ...
Vigna radiata var. radiata
Plant Physiol.
97
396-401
1991
-
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2
2
1
2
1
1
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3
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1
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1
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2
1
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1
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1
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2
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2
1
2
1
1
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1
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1
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2
1
1
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1
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488487
Miernyk
-
Characterization of maize endo ...
Zea mays
Phytochemistry
30
2865-2867
1991
1
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4
3
1
1
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1
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1
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1
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1
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4
3
3
1
1
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1
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1
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1
1
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1
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488484
Matern
Isolation and characterization ...
Homo sapiens
Eur. J. Biochem.
190
99-105
1990
1
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1
1
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1
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1
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2
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488485
Matern
Control of dolichyl phosphoglu ...
Homo sapiens
Biochim. Biophys. Acta
1004
67-72
1989
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1
2
1
4
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1
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2
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488483
Riedell
Glycoprotein synthesis in maiz ...
Zea mays
Plant Physiol.
87
420-426
1988
2
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3
1
1
5
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1
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5
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488480
Villemez
Properties of a soluble polypr ...
Acanthamoeba castellanii
J. Biol. Chem.
254
4814-4819
1979
1
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3
2
1
2
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2
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1
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2
6
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1
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2
6
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1
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2
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1
1
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488481
Herscovics
Glucosyltransferase activity i ...
Bos taurus
J. Biol. Chem.
252
2271-2277
1977
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1
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488482
Behrens
Dolichol monophosphate glucose ...
Rattus norvegicus
Proc. Natl. Acad. Sci. USA
66
153-159
1970
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