BRENDA - Enzyme Database
show all sequences of 2.4.1.117

Control of dolichyl phosphoglucose formation in human liver microsomes. Kinetic and inhibition studies of nucleosides, nucleotides and analogues of UDPglucose

Matern, H.; Matern, S.; Biochim. Biophys. Acta 1004, 67-72 (1989)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
UDP
-
Homo sapiens
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.00015
-
UDP-glucose
pH 7.2
Homo sapiens
0.00033
-
UDP-glucose
pH 5.3
Homo sapiens
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
-
Homo sapiens
-
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Ca2+
divalent cation required, maximum stimulation in presence of Ca2+. Co2+, Mg2+ or Mn2+ can replace Ca2+
Homo sapiens
Co2+
divalent cation required, maximum stimulation in presence of Ca2+. Co2+, Mg2+ or Mn2+ can replace Ca2+
Homo sapiens
Mg2+
divalent cation required, maximum stimulation in presence of Ca2+. Co2+, Mg2+ or Mn2+ can replace Ca2+
Homo sapiens
Mn2+
divalent cation required, maximum stimulation in presence of Ca2+. Co2+, Mg2+ or Mn2+ can replace Ca2+
Homo sapiens
Organism
Organism
UniProt
Commentary
Textmining
Homo sapiens
-
-
-
Reaction
Reaction
Commentary
Organism
Reaction ID
UDP-alpha-D-glucose + dolichyl phosphate = UDP + dolichyl beta-D-glucosyl phosphate
sequential mechanism
Homo sapiens
Source Tissue
Source Tissue
Commentary
Organism
Textmining
liver
-
Homo sapiens
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
UDP-glucose + dolichyl phosphate
-
488485
Homo sapiens
UDP + dolichyl beta-D-glucosyl phosphate
-
-
-
?
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.3
-
second optimum at pH 7.2
Homo sapiens
7.2
-
second optimum at pH 5.3
Homo sapiens
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.1
-
UDP
at pH 7.2
Homo sapiens
0.17
-
UDP
at pH 5.3
Homo sapiens
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
UDP
-
Homo sapiens
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.1
-
UDP
at pH 7.2
Homo sapiens
0.17
-
UDP
at pH 5.3
Homo sapiens
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.00015
-
UDP-glucose
pH 7.2
Homo sapiens
0.00033
-
UDP-glucose
pH 5.3
Homo sapiens
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
-
Homo sapiens
-
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Ca2+
divalent cation required, maximum stimulation in presence of Ca2+. Co2+, Mg2+ or Mn2+ can replace Ca2+
Homo sapiens
Co2+
divalent cation required, maximum stimulation in presence of Ca2+. Co2+, Mg2+ or Mn2+ can replace Ca2+
Homo sapiens
Mg2+
divalent cation required, maximum stimulation in presence of Ca2+. Co2+, Mg2+ or Mn2+ can replace Ca2+
Homo sapiens
Mn2+
divalent cation required, maximum stimulation in presence of Ca2+. Co2+, Mg2+ or Mn2+ can replace Ca2+
Homo sapiens
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
liver
-
Homo sapiens
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
UDP-glucose + dolichyl phosphate
-
488485
Homo sapiens
UDP + dolichyl beta-D-glucosyl phosphate
-
-
-
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.3
-
second optimum at pH 7.2
Homo sapiens
7.2
-
second optimum at pH 5.3
Homo sapiens
Other publictions for EC 2.4.1.117
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
686298
Haecker
Wollknauel is required for emb ...
Drosophila sp. (in: Insecta)
Development
135
1745-1749
2008
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488488
Arroyo-Flores
Biosynthesis of glycoproteins ...
Candida albicans
Fungal Genet. Biol.
30
127-133
2000
-
-
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-
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4
1
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3
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1
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1
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488493
Lopez-Romero
-
Glycosyl transferases and glyc ...
Candida albicans
Recent Res. Dev. Microbiol.
4
667-681
2000
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488492
Rodriguez-Bonilla
Biosynthesis of glycoproteins ...
Candida albicans
Antonie van Leeuwenhoek
73
373-380
1998
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-
-
-
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-
1
1
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3
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1
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1
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1
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488489
Gartung
The submicrosomal localization ...
Homo sapiens
J. Hepatol.
20
32-40
1994
-
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1
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1
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488491
Heesen
Isolation of the ALG5 locus en ...
Saccharomyces cerevisiae
Eur. J. Biochem.
224
71-79
1994
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-
1
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1
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3
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1
1
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488490
Bossuyt
-
Topology of nucleotide-sugar:d ...
Rattus norvegicus
Biochem. J.
296
627-632
1993
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-
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1
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1
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488486
Drake
Partial purificaton, photoaffi ...
Vigna radiata var. radiata
Plant Physiol.
97
396-401
1991
-
-
-
-
-
-
2
2
1
2
1
1
-
3
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1
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1
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1
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1
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488487
Miernyk
-
Characterization of maize endo ...
Zea mays
Phytochemistry
30
2865-2867
1991
1
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4
3
1
1
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1
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1
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488484
Matern
Isolation and characterization ...
Homo sapiens
Eur. J. Biochem.
190
99-105
1990
1
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1
1
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1
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488485
Matern
Control of dolichyl phosphoglu ...
Homo sapiens
Biochim. Biophys. Acta
1004
67-72
1989
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1
2
1
4
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1
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2
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488483
Riedell
Glycoprotein synthesis in maiz ...
Zea mays
Plant Physiol.
87
420-426
1988
2
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3
1
1
5
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1
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2
1
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5
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488480
Villemez
Properties of a soluble polypr ...
Acanthamoeba castellanii
J. Biol. Chem.
254
4814-4819
1979
1
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3
2
1
2
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1
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6
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6
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1
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488481
Herscovics
Glucosyltransferase activity i ...
Bos taurus
J. Biol. Chem.
252
2271-2277
1977
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488482
Behrens
Dolichol monophosphate glucose ...
Rattus norvegicus
Proc. Natl. Acad. Sci. USA
66
153-159
1970
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