BRENDA - Enzyme Database
show all sequences of 2.4.1.117

Properties of a soluble polyprenyl phosphate: UDP-D-glucose glucosyltransferase

Villemez, C.L.; Carlo, P.L.; J. Biol. Chem. 254, 4814-4819 (1979)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
Reducing agent
required
Acanthamoeba castellanii
Inhibitors
Inhibitors
Commentary
Organism
Structure
EDTA
inhibition reversed by MgCl2
Acanthamoeba castellanii
UDP
competitive
Acanthamoeba castellanii
UMP
reversible
Acanthamoeba castellanii
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0045
-
dolichyl phosphate
-
Acanthamoeba castellanii
0.0091
-
UDP-glucose
-
Acanthamoeba castellanii
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
soluble
-
Acanthamoeba castellanii
-
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
divalent cation required, either Mg2+ or Mn2+, Mn2+ stimulates the enzyme to a greater extent than Mg2+ at low concentrations, Mg2+ stimulates to a greater extent than Mn2+ at high concentration, but each is maximally effective at a concentration around 8 mM
Acanthamoeba castellanii
Mn2+
divalent cation required, either Mg2+ or Mn2+, Mn2+ stimulates the enzyme to a greater extent than Mg2+ at low concentrations, Mg2+ stimulates to a greater extent than Mn2+ at high concentration, but each is maximally effective at a concentration around 8 mM
Acanthamoeba castellanii
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Acanthamoeba castellanii
synthesis of dolichyl D-glucosyl phosphate, a glucosyl donor in the formation of lipid-linked core oligosaccharides
?
-
-
-
UDP-glucose + dolichyl phosphate
Acanthamoeba castellanii
-
UDP + dolichyl beta-D-glucosyl phosphate
-
-
r
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Acanthamoeba castellanii
-
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
cyst
-
Acanthamoeba castellanii
-
Storage Stability
Storage Stability
Organism
-20°C, indefinitely stable below
Acanthamoeba castellanii
4°C, stable for several days
Acanthamoeba castellanii
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
no substrate: GDP-D-glucose, UDP-D-glucuronic acid, UDP-N-acetyl-D-glucosamine, UDP-D-xylose
488480
Acanthamoeba castellanii
?
-
-
-
-
additional information
synthesis of dolichyl D-glucosyl phosphate, a glucosyl donor in the formation of lipid-linked core oligosaccharides
488480
Acanthamoeba castellanii
?
-
-
-
-
UDP-glucose + dolichyl phosphate
-
488480
Acanthamoeba castellanii
UDP + dolichyl beta-D-glucosyl phosphate
-
-
-
r
UDP-glucose + dolichyl phosphate
-
488480
Acanthamoeba castellanii
UDP + dolichyl beta-D-glucosyl phosphate
-
488480
Acanthamoeba castellanii
r
UDP-glucose + ficaprenyl phosphate
-
488480
Acanthamoeba castellanii
UDP + ficaprenyl beta-D-glucosyl phosphate
-
-
-
r
UDP-glucose + solanesyl phosphate
-
488480
Acanthamoeba castellanii
UDP + solanesyl beta-D-glucosyl phosphate
-
-
-
r
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
-
Acanthamoeba castellanii
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
30
-
2 h, 50% loss of activity
Acanthamoeba castellanii
52
-
10 min, complete loss of activity
Acanthamoeba castellanii
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
-
Acanthamoeba castellanii
pH Range
pH Minimum
pH Maximum
Commentary
Organism
5.6
8.8
pH 5.6: 25% of maximum activity, pH 8.8: about 40% of maximum activity
Acanthamoeba castellanii
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
Reducing agent
required
Acanthamoeba castellanii
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
EDTA
inhibition reversed by MgCl2
Acanthamoeba castellanii
UDP
competitive
Acanthamoeba castellanii
UMP
reversible
Acanthamoeba castellanii
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0045
-
dolichyl phosphate
-
Acanthamoeba castellanii
0.0091
-
UDP-glucose
-
Acanthamoeba castellanii
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
soluble
-
Acanthamoeba castellanii
-
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
divalent cation required, either Mg2+ or Mn2+, Mn2+ stimulates the enzyme to a greater extent than Mg2+ at low concentrations, Mg2+ stimulates to a greater extent than Mn2+ at high concentration, but each is maximally effective at a concentration around 8 mM
Acanthamoeba castellanii
Mn2+
divalent cation required, either Mg2+ or Mn2+, Mn2+ stimulates the enzyme to a greater extent than Mg2+ at low concentrations, Mg2+ stimulates to a greater extent than Mn2+ at high concentration, but each is maximally effective at a concentration around 8 mM
Acanthamoeba castellanii
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Acanthamoeba castellanii
synthesis of dolichyl D-glucosyl phosphate, a glucosyl donor in the formation of lipid-linked core oligosaccharides
?
-
-
-
UDP-glucose + dolichyl phosphate
Acanthamoeba castellanii
-
UDP + dolichyl beta-D-glucosyl phosphate
-
-
r
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
cyst
-
Acanthamoeba castellanii
-
Storage Stability (protein specific)
Storage Stability
Organism
-20°C, indefinitely stable below
Acanthamoeba castellanii
4°C, stable for several days
Acanthamoeba castellanii
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
no substrate: GDP-D-glucose, UDP-D-glucuronic acid, UDP-N-acetyl-D-glucosamine, UDP-D-xylose
488480
Acanthamoeba castellanii
?
-
-
-
-
additional information
synthesis of dolichyl D-glucosyl phosphate, a glucosyl donor in the formation of lipid-linked core oligosaccharides
488480
Acanthamoeba castellanii
?
-
-
-
-
UDP-glucose + dolichyl phosphate
-
488480
Acanthamoeba castellanii
UDP + dolichyl beta-D-glucosyl phosphate
-
-
-
r
UDP-glucose + dolichyl phosphate
-
488480
Acanthamoeba castellanii
UDP + dolichyl beta-D-glucosyl phosphate
-
488480
Acanthamoeba castellanii
r
UDP-glucose + ficaprenyl phosphate
-
488480
Acanthamoeba castellanii
UDP + ficaprenyl beta-D-glucosyl phosphate
-
-
-
r
UDP-glucose + solanesyl phosphate
-
488480
Acanthamoeba castellanii
UDP + solanesyl beta-D-glucosyl phosphate
-
-
-
r
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
-
Acanthamoeba castellanii
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
30
-
2 h, 50% loss of activity
Acanthamoeba castellanii
52
-
10 min, complete loss of activity
Acanthamoeba castellanii
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
-
Acanthamoeba castellanii
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
5.6
8.8
pH 5.6: 25% of maximum activity, pH 8.8: about 40% of maximum activity
Acanthamoeba castellanii
Other publictions for EC 2.4.1.117
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
686298
Haecker
Wollknauel is required for emb ...
Drosophila sp. (in: Insecta)
Development
135
1745-1749
2008
-
-
-
-
-
-
-
-
-
-
-
1
-
3
-
-
-
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-
1
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1
-
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-
-
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1
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1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
488488
Arroyo-Flores
Biosynthesis of glycoproteins ...
Candida albicans
Fungal Genet. Biol.
30
127-133
2000
-
-
-
-
-
-
4
1
-
3
-
-
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
1
-
-
-
1
-
1
-
-
-
-
-
-
-
1
4
1
1
-
3
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
488493
Lopez-Romero
-
Glycosyl transferases and glyc ...
Candida albicans
Recent Res. Dev. Microbiol.
4
667-681
2000
-
-
-
-
-
-
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1
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-
-
-
-
-
488492
Rodriguez-Bonilla
Biosynthesis of glycoproteins ...
Candida albicans
Antonie van Leeuwenhoek
73
373-380
1998
-
-
-
-
-
-
1
1
-
3
-
-
-
1
-
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1
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1
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1
1
1
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3
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1
-
-
-
-
-
-
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-
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-
-
488489
Gartung
The submicrosomal localization ...
Homo sapiens
J. Hepatol.
20
32-40
1994
-
-
-
-
-
-
-
-
1
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1
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1
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1
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1
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-
-
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-
-
-
-
488491
Heesen
Isolation of the ALG5 locus en ...
Saccharomyces cerevisiae
Eur. J. Biochem.
224
71-79
1994
-
-
1
-
-
-
-
-
1
-
1
-
-
3
-
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-
-
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1
1
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1
-
-
-
-
-
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1
-
1
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
488490
Bossuyt
-
Topology of nucleotide-sugar:d ...
Rattus norvegicus
Biochem. J.
296
627-632
1993
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
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1
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-
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-
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-
488486
Drake
Partial purificaton, photoaffi ...
Vigna radiata var. radiata
Plant Physiol.
97
396-401
1991
-
-
-
-
-
-
2
2
1
2
1
1
-
3
-
-
1
-
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-
1
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2
1
1
-
-
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1
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2
-
2
1
2
1
1
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1
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1
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2
1
1
-
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1
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488487
Miernyk
-
Characterization of maize endo ...
Zea mays
Phytochemistry
30
2865-2867
1991
1
-
-
-
-
-
4
3
1
1
-
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1
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1
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1
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1
1
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1
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3
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1
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4
3
3
1
1
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1
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1
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1
1
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1
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-
488484
Matern
Isolation and characterization ...
Homo sapiens
Eur. J. Biochem.
190
99-105
1990
1
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-
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-
1
1
-
1
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1
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2
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1
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1
2
1
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1
1
2
1
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1
1
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-
-
488485
Matern
Control of dolichyl phosphoglu ...
Homo sapiens
Biochim. Biophys. Acta
1004
67-72
1989
-
-
-
-
-
-
1
2
1
4
-
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1
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1
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1
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1
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1
4
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1
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1
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2
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488483
Riedell
Glycoprotein synthesis in maiz ...
Zea mays
Plant Physiol.
87
420-426
1988
2
-
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3
1
1
5
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1
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2
1
1
5
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1
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488480
Villemez
Properties of a soluble polypr ...
Acanthamoeba castellanii
J. Biol. Chem.
254
4814-4819
1979
1
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-
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3
2
1
2
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2
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1
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1
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2
6
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1
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2
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1
1
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1
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3
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2
1
2
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2
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1
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2
6
-
1
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2
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1
1
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-
488481
Herscovics
Glucosyltransferase activity i ...
Bos taurus
J. Biol. Chem.
252
2271-2277
1977
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1
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1
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488482
Behrens
Dolichol monophosphate glucose ...
Rattus norvegicus
Proc. Natl. Acad. Sci. USA
66
153-159
1970
-
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-
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1
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1
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1
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1
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1
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1
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1
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